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Muthupandian Saravanan
Mekelle University College of Health Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1480-3555
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In the current study, silver chloride nanoparticles (AgClNPs) have been synthesized using the aqueous extract of Padina gymnospora and further characterized by Uv-visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction, Scanning electron microscopy, energy dispersive spectroscopy, Transmission electron microscopy and Atomic force microscopy. The synthesized silver chloride nanoparticles were found to be mono-dispersed and spherical with an average size between 11.5 to 32.86 nm. The particles also showed a cytotoxic effect in a dose-dependent manner against MCF 7 cells (IC50 = 31.37 µg/mL). Besides, it showed the larvicidal activity against Aedes aegypti at a lower dose (3.92 µg/ml) than that of the aqueous extract (13.01 µg/ml). It also exhibited significant antimicrobial activity against selected bacterial and fungal pathogens. The synthesized silver chloride nanoparticles showed the best minimum inhibitory concentration (MIC) of 8 mg/L in Canadian Albicans, followed by vancomycin resistance Enterococcus feacasli (VREF) 32 mg/L. Furthermore, the toxicity assessment by haemolytic assay revealed that AgClNPs could be safe and also used for further investigation. Therefore, the synthesized silver chloride nanoparticles may be used as a therapeutic agent for breast cancer, antimicrobial agent and also to control the dengue vector as attributed to the presence of bioactive components.
Keywords
Padina gymnospora, Silver chloride nanoparticles, Human breast cancer, Aedes aegypti, antimicrobial, larvicidal, Cytotoxicity, Flow cytometry
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research
Muthupandian Saravanan
Mekelle University College of Health Sciences
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1480-3555
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 13 May, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Nanoscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
In the current study, silver chloride nanoparticles (AgClNPs) have been synthesized using the aqueous extract of Padina gymnospora and further characterized by Uv-visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction, Scanning electron microscopy, energy dispersive spectroscopy, Transmission electron microscopy and Atomic force microscopy. The synthesized silver chloride nanoparticles were found to be mono-dispersed and spherical with an average size between 11.5 to 32.86 nm. The particles also showed a cytotoxic effect in a dose-dependent manner against MCF 7 cells (IC50 = 31.37 µg/mL). Besides, it showed the larvicidal activity against Aedes aegypti at a lower dose (3.92 µg/ml) than that of the aqueous extract (13.01 µg/ml). It also exhibited significant antimicrobial activity against selected bacterial and fungal pathogens. The synthesized silver chloride nanoparticles showed the best minimum inhibitory concentration (MIC) of 8 mg/L in Canadian Albicans, followed by vancomycin resistance Enterococcus feacasli (VREF) 32 mg/L. Furthermore, the toxicity assessment by haemolytic assay revealed that AgClNPs could be safe and also used for further investigation. Therefore, the synthesized silver chloride nanoparticles may be used as a therapeutic agent for breast cancer, antimicrobial agent and also to control the dengue vector as attributed to the presence of bioactive components.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
This is a list of supplementary files associated with this preprint. Click to download.
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