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Short report
Intraventricular IL-17A administration activates microglia and alters their localization in the mouse embryo cerebral cortex
Tetsuya Sasaki
Tsukuba Daigaku Igaku Iryokei
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7723-4417
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Molecular Brain.
Viral infection during pregnancy has been suggested to increase the probability of autism spectrum disorder (ASD) in offspring via the phenomenon of maternal immune activation (MIA). This has been modeled in rodents. Maternal T helper 17 cells and the effector cytokine, interleukin 17A (IL-17A), play a central role in MIA-induced behavioral abnormalities and cortical dysgenesis, termed cortical patch. However, it is unclear how IL-17A acts on fetal brain cells to cause ASD pathologies. To assess the effect of IL-17A on cortical development, we directly administered IL-17A into the lateral ventricles of the fetal mouse brain. We analyzed injected brains focusing on microglia, which express IL-17A receptors. We found that IL-17A activated microglia and altered their localization in the cerebral cortex. Our data indicate that IL-17A activates cortical microglia, which leads to a cascade of ASD-related brain pathologies, including excessive phagocytosis of neural progenitor cells in the ventricular zone.
Keywords
Autism Spectrum Disorder, CD68, Corticogenesis, IL-17A, Microglia
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CURRENT STATUS: Published
Version 2
Posted 03 Jun, 2020
Published
On 16 Jun, 2020
No community comments so far
Editorial decision: Accept
On 03 Jun, 2020
Review #1 received
Received 31 May, 2020
Reviewer #1 agreed
On 28 May, 2020
Reviewers invited
Invitations sent on 28 May, 2020
Editor assigned
On 26 May, 2020
Submission checks complete
On 25 May, 2020
Editor invited
On 25 May, 2020
No comments provided
Review #2 received
Received 15 May, 2020
Editorial decision: Minor revision
On 15 May, 2020
Review #1 received
Received 06 May, 2020
Reviewer #2 agreed
On 02 May, 2020
Reviewers invited
Invitations sent on 02 May, 2020
Reviewer #1 agreed
On 02 May, 2020
Editor assigned
On 28 Apr, 2020
Submission checks complete
On 27 Apr, 2020
Editor invited
On 27 Apr, 2020
First submitted
On 24 Apr, 2020
Metrics
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PDF Downloads: ...
HTML Views: ...
Subject Areas
Cellular & Molecular Neuroscience
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This preprint is under consideration at Molecular Brain. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Short report
Intraventricular IL-17A administration activates microglia and alters their localization in the mouse embryo cerebral cortex
Tetsuya Sasaki
Tsukuba Daigaku Igaku Iryokei
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7723-4417
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 03 Jun, 2020
Published
On 16 Jun, 2020
No community comments so far
Editorial decision: Accept
On 03 Jun, 2020
Review #1 received
Received 31 May, 2020
Reviewer #1 agreed
On 28 May, 2020
Reviewers invited
Invitations sent on 28 May, 2020
Editor assigned
On 26 May, 2020
Submission checks complete
On 25 May, 2020
Editor invited
On 25 May, 2020
No comments provided
Review #2 received
Received 15 May, 2020
Editorial decision: Minor revision
On 15 May, 2020
Review #1 received
Received 06 May, 2020
Reviewer #2 agreed
On 02 May, 2020
Reviewers invited
Invitations sent on 02 May, 2020
Reviewer #1 agreed
On 02 May, 2020
Editor assigned
On 28 Apr, 2020
Submission checks complete
On 27 Apr, 2020
Editor invited
On 27 Apr, 2020
First submitted
On 24 Apr, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cellular & Molecular Neuroscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Molecular Brain.
Viral infection during pregnancy has been suggested to increase the probability of autism spectrum disorder (ASD) in offspring via the phenomenon of maternal immune activation (MIA). This has been modeled in rodents. Maternal T helper 17 cells and the effector cytokine, interleukin 17A (IL-17A), play a central role in MIA-induced behavioral abnormalities and cortical dysgenesis, termed cortical patch. However, it is unclear how IL-17A acts on fetal brain cells to cause ASD pathologies. To assess the effect of IL-17A on cortical development, we directly administered IL-17A into the lateral ventricles of the fetal mouse brain. We analyzed injected brains focusing on microglia, which express IL-17A receptors. We found that IL-17A activated microglia and altered their localization in the cerebral cortex. Our data indicate that IL-17A activates cortical microglia, which leads to a cascade of ASD-related brain pathologies, including excessive phagocytosis of neural progenitor cells in the ventricular zone.
Figure 1
Figure 2
Figure 3
Figure 4