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Research article
Xiaoyue Yu
China national rice research institute
Corresponding Author
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Background
Herbicide resistant mutations are predicted to exhibit fitness cost under herbicide-free conditions. Asia minor bluegrass (Polypogon fugax) is a common weed species in the winter crops. Our previous study established a P. fugax accession (LR) resistant to acetyl-CoA carboxylase (ACCase) inhibiting herbicides. Besides, LR also exhibited fitness cost, like lower germination, relative to the sensitive plants (LS). But little is known about the gene expression profile of seed germination process in herbicide resistance P.fugax.
Results
The maximum germination (gMAX) of LS reached 94%, while that of LR was 52%. Exogenous gibberellin and abscisic acid synthesis inhibitor fluridone could break the dormancy of LR, increasing gMAX to 78% and 94%, respectively. A comparative transcriptome was conducted to analyze the genes expression profile of LS and LR at two germination time points. A total of 11,856 and 23,123 differentially expressed genes (DEGs) were identified in LS and LR at two time points. Most of DEGs were involved in lipid metabolism, carbohydrate metabolism, amino acid metabolism and secondary metabolites biosynthesis. Four genes related to GA biosynthesis and signal transduction showed higher expression in LS and five genes related to ABA biosynthesis and signal transduction showed higher expression in LR during germination. Twenty-four genes involved in glycolysis, TCA cycle, pentose phosphate pathway and fatty acid metabolism had higher expression levels in LS than LR during germination.
Conclusions
Our results indicated that GA biosynthesis and transduction, carbohydrate and fatty acid metabolism was more actively expressed in LS plants, resulting in stronger germination ability of LS relative to LR. The highly expressed genes in ABA biosynthesis and transduction contribute to maintain the seed dormancy of LR plants. This study provided new insight into transcriptional changes and interaction in seed germination process of P. fugax, and compared the differential expression profile between the herbicides resistant and sensitive plants during seed germination.
Keywords
seed germination, Polypogan fugax, herbicide resistance, transcriptome
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This preprint is under consideration at BMC Genomics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Xiaoyue Yu
China national rice research institute
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 02 Jun, 2020
No community comments so far
Editor assigned
On 05 May, 2020
Submission checks complete
On 04 May, 2020
Editor invited
On 04 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Herbicide resistant mutations are predicted to exhibit fitness cost under herbicide-free conditions. Asia minor bluegrass (Polypogon fugax) is a common weed species in the winter crops. Our previous study established a P. fugax accession (LR) resistant to acetyl-CoA carboxylase (ACCase) inhibiting herbicides. Besides, LR also exhibited fitness cost, like lower germination, relative to the sensitive plants (LS). But little is known about the gene expression profile of seed germination process in herbicide resistance P.fugax.
Results
The maximum germination (gMAX) of LS reached 94%, while that of LR was 52%. Exogenous gibberellin and abscisic acid synthesis inhibitor fluridone could break the dormancy of LR, increasing gMAX to 78% and 94%, respectively. A comparative transcriptome was conducted to analyze the genes expression profile of LS and LR at two germination time points. A total of 11,856 and 23,123 differentially expressed genes (DEGs) were identified in LS and LR at two time points. Most of DEGs were involved in lipid metabolism, carbohydrate metabolism, amino acid metabolism and secondary metabolites biosynthesis. Four genes related to GA biosynthesis and signal transduction showed higher expression in LS and five genes related to ABA biosynthesis and signal transduction showed higher expression in LR during germination. Twenty-four genes involved in glycolysis, TCA cycle, pentose phosphate pathway and fatty acid metabolism had higher expression levels in LS than LR during germination.
Conclusions
Our results indicated that GA biosynthesis and transduction, carbohydrate and fatty acid metabolism was more actively expressed in LS plants, resulting in stronger germination ability of LS relative to LR. The highly expressed genes in ABA biosynthesis and transduction contribute to maintain the seed dormancy of LR plants. This study provided new insight into transcriptional changes and interaction in seed germination process of P. fugax, and compared the differential expression profile between the herbicides resistant and sensitive plants during seed germination.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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