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Purpose: Truffles contribute to crucial dynamics in the soil systems, being involved in plentiful ecological functions important for ecosystems. Despite this, the interactions between truffles and surrounding mycobiota remain unknown. Here, we aimed to shed light on how much truffle species could affect their surrounding soil mycobiota.
Methods: Using traditional chemical analysis and ITS Illumina sequencing, we compared soil nutrients and the mycobiota in soil, gleba and peridium of the two truffle species, Tuber indicum (Ti) and T. pseudohimalayense (Tp), inhabiting the same Pinus armandii forest in southwestern China.
Result: Tp soil was more acidic and had higher nutrients (total C, N contents) than Ti soil. Fungal richness and diversity of ascomata tissues and surrounding soils were significantly higher in Tp than in Ti. Truffle species recruited unique soil mycobiota around its ascomata: in Ti soil, fungal taxa including Suillus, Alternaria, Phacidium, Mycosphaerella, Halokirschsteiniothelia, Pseudogymnoascus were abundant while in Tp soil the species of Melanophyllum, Inocybe, Rhizopogon, Rhacidium, and Lecanicillium showed higher abundances. Three dissimilarity tests including adonis, anosim and mrpp showed the differences of fungal community structure between the truffle and their surrounding soil was stronger in Tp than in Ti, and such differences extended to truffle tissues (peridium and gleba). Redundancy analysis further demonstrated that relationships between soil fungal taxa and soil properties had changed from negative (Tp) to positive (Ti) and shifted from a moisture-driving (Tp) to a total N-driving (Ti).
Conclusion: Overall, our results showed that the different interactions between truffle and soil system although the causative peculiarity of these associations needs to be further studied.
Keywords
hypogenous ectomycorrhizal fungi, truffles, soil nutrient, fungal community, mycobiota
Figure 1
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Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryTableandFigures.docx
Table S1. High throughput sequence parameters in the control and truffle surrounding soils, and fungal tissues of Tuber indicum and T. pseudohymalyense occurring in a P. armandii forest. Figure S1. Spearman co-relationships between fungal taxa (top 30 genera) and soil properties in the fungal tissues of T. pseudohymalyense associated with P. armandii. Red and green color indicates positive and negative co-relationship, respectively. *** P < 0.001. Figure S2. Spearman co-relationships between fungal taxa (top 30 genera) and soil properties in the fungal tissues of T. indicum associated with P. armandii. Red and green color indicates positive and negative co-relationship, respectively. *** P < 0.001. Figure S3. Spearman co-relationships between fungal diversity indices and soil properties in the fungal tissues of T. indicum associated with P. armandii. Red and green color indicates positive and negative co-relationship, respectively. ** P < 0.01, * P < 0.05.
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CURRENT STATUS: Under Review
Version 1
Posted 10 Mar, 2021
No community comments so far
Reviews received
Received 13 Apr, 2021
Reviewers invited
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Editor invited
On 19 Feb, 2021
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On 17 Feb, 2021
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Subject Areas
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This preprint is under consideration at Archives of Microbiology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
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Research Article
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 10 Mar, 2021
No community comments so far
Reviews received
Received 13 Apr, 2021
Reviewers invited
Invitations sent on 01 Mar, 2021
Editor invited
On 19 Feb, 2021
Editor assigned
On 17 Feb, 2021
First submitted
On 16 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Purpose: Truffles contribute to crucial dynamics in the soil systems, being involved in plentiful ecological functions important for ecosystems. Despite this, the interactions between truffles and surrounding mycobiota remain unknown. Here, we aimed to shed light on how much truffle species could affect their surrounding soil mycobiota.
Methods: Using traditional chemical analysis and ITS Illumina sequencing, we compared soil nutrients and the mycobiota in soil, gleba and peridium of the two truffle species, Tuber indicum (Ti) and T. pseudohimalayense (Tp), inhabiting the same Pinus armandii forest in southwestern China.
Result: Tp soil was more acidic and had higher nutrients (total C, N contents) than Ti soil. Fungal richness and diversity of ascomata tissues and surrounding soils were significantly higher in Tp than in Ti. Truffle species recruited unique soil mycobiota around its ascomata: in Ti soil, fungal taxa including Suillus, Alternaria, Phacidium, Mycosphaerella, Halokirschsteiniothelia, Pseudogymnoascus were abundant while in Tp soil the species of Melanophyllum, Inocybe, Rhizopogon, Rhacidium, and Lecanicillium showed higher abundances. Three dissimilarity tests including adonis, anosim and mrpp showed the differences of fungal community structure between the truffle and their surrounding soil was stronger in Tp than in Ti, and such differences extended to truffle tissues (peridium and gleba). Redundancy analysis further demonstrated that relationships between soil fungal taxa and soil properties had changed from negative (Tp) to positive (Ti) and shifted from a moisture-driving (Tp) to a total N-driving (Ti).
Conclusion: Overall, our results showed that the different interactions between truffle and soil system although the causative peculiarity of these associations needs to be further studied.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
- SupplementaryTableandFigures.docx
Table S1. High throughput sequence parameters in the control and truffle surrounding soils, and fungal tissues of Tuber indicum and T. pseudohymalyense occurring in a P. armandii forest. Figure S1. Spearman co-relationships between fungal taxa (top 30 genera) and soil properties in the fungal tissues of T. pseudohymalyense associated with P. armandii. Red and green color indicates positive and negative co-relationship, respectively. *** P < 0.001. Figure S2. Spearman co-relationships between fungal taxa (top 30 genera) and soil properties in the fungal tissues of T. indicum associated with P. armandii. Red and green color indicates positive and negative co-relationship, respectively. *** P < 0.001. Figure S3. Spearman co-relationships between fungal diversity indices and soil properties in the fungal tissues of T. indicum associated with P. armandii. Red and green color indicates positive and negative co-relationship, respectively. ** P < 0.01, * P < 0.05.
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