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Research article
Marie Margarete Meyerholz
Ludwig-Maximilians-Universitat Munchen
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1720-6593
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: In dairy herds, mastitis causes detrimental economic losses. Genetic selection offers a sustainable tool to select animals with reduced susceptibility towards postpartum diseases. Studying underlying mechanisms is important to assess the physiological processes that cause differences between selected haplotypes. Therefore, the objective of this study was to establish an in vivo infection model to study the impact of selecting for alternative paternal haplotypes in a particular genomic region on cattle chromosome 18 for mastitis susceptibility under defined conditions in dairy uniparous cows.
Results: At the start of pathogen challenge, no significant differences between the favorable (Q) and unfavorable (q) haplotypes were detected. Intramammary infection (IMI) with Staphylococcus aureus 1027 (S. aureus, n = 24, 96 h) or Escherichia coli 1303 (E. coli, n = 12, 24 h) was successfully induced in all uniparous cows. This finding was confirmed by clinical signs of mastitis and repeated recovery of the respective pathogen from milk samples of challenged quarters in each animal. After S. aureus challenge, Q-uniparous cows showed lower somatic cell counts 24 h and 36 h after challenge (P < 0.05), lower bacterial shedding in milk 12 h after challenge (P < 0.01) and a minor decrease in total milk yield 12 h and 24 h after challenge (P < 0.01) compared to q-uniparous cows.
Conclusion: An in vivo infection model to study the impact of genetic selection for mastitis susceptibility under defined conditions in dairy uniparous cows was successfully established and revealed significant differences between the two genetically selected haplotype groups. This result might explain their differences in susceptibility towards IMI. These clinical findings form the basis for further in-depth molecular analysis to clarify the underlying genetic mechanisms for mastitis resistance.
Keywords
Staphylococcus aureus, Escherichia coli, BTA18, genetic selection, haplotype, intramammary infection model, somatic cell count, mastitis
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CURRENT STATUS: Published
View the published article at BMC Veterinary Research.
Version 3
Posted 21 Jan, 2020
No community comments so far
Submission checks complete
On 18 Jan, 2020
Editorial decision: Accept
On 18 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 10 Jan, 2020
Review #2 received
Received 03 Dec, 2019
Reviewer #1 agreed
On 03 Dec, 2019
Reviewer #2 agreed
On 03 Dec, 2019
Review #1 received
Received 03 Dec, 2019
Editor assigned
On 02 Dec, 2019
Reviewers invited
Invitations sent on 02 Dec, 2019
Submission checks complete
On 01 Dec, 2019
Editor invited
On 01 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 26 Oct, 2019
Review #2 received
Received 24 Oct, 2019
Reviewer #2 agreed
On 21 Aug, 2019
Review #1 received
Received 30 Jul, 2019
Reviewer #1 agreed
On 16 Jul, 2019
Submission checks complete
On 16 Jul, 2019
Editor invited
On 16 Jul, 2019
Editor assigned
On 16 Jul, 2019
Reviewers invited
Invitations sent on 16 Jul, 2019
First submitted
On 05 Jul, 2019
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See the published version of this preprint at BMC Veterinary Research.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Marie Margarete Meyerholz
Ludwig-Maximilians-Universitat Munchen
Corresponding Author
ORCiD: https://orcid.org/0000-0003-1720-6593
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Veterinary Research.
Version 3
Posted 21 Jan, 2020
No community comments so far
Submission checks complete
On 18 Jan, 2020
Editorial decision: Accept
On 18 Jan, 2020
No comments provided
Editorial decision: Minor revision
On 10 Jan, 2020
Review #2 received
Received 03 Dec, 2019
Reviewer #1 agreed
On 03 Dec, 2019
Reviewer #2 agreed
On 03 Dec, 2019
Review #1 received
Received 03 Dec, 2019
Editor assigned
On 02 Dec, 2019
Reviewers invited
Invitations sent on 02 Dec, 2019
Submission checks complete
On 01 Dec, 2019
Editor invited
On 01 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 26 Oct, 2019
Review #2 received
Received 24 Oct, 2019
Reviewer #2 agreed
On 21 Aug, 2019
Review #1 received
Received 30 Jul, 2019
Reviewer #1 agreed
On 16 Jul, 2019
Submission checks complete
On 16 Jul, 2019
Editor invited
On 16 Jul, 2019
Editor assigned
On 16 Jul, 2019
Reviewers invited
Invitations sent on 16 Jul, 2019
First submitted
On 05 Jul, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Veterinary Research.
Background: In dairy herds, mastitis causes detrimental economic losses. Genetic selection offers a sustainable tool to select animals with reduced susceptibility towards postpartum diseases. Studying underlying mechanisms is important to assess the physiological processes that cause differences between selected haplotypes. Therefore, the objective of this study was to establish an in vivo infection model to study the impact of selecting for alternative paternal haplotypes in a particular genomic region on cattle chromosome 18 for mastitis susceptibility under defined conditions in dairy uniparous cows.
Results: At the start of pathogen challenge, no significant differences between the favorable (Q) and unfavorable (q) haplotypes were detected. Intramammary infection (IMI) with Staphylococcus aureus 1027 (S. aureus, n = 24, 96 h) or Escherichia coli 1303 (E. coli, n = 12, 24 h) was successfully induced in all uniparous cows. This finding was confirmed by clinical signs of mastitis and repeated recovery of the respective pathogen from milk samples of challenged quarters in each animal. After S. aureus challenge, Q-uniparous cows showed lower somatic cell counts 24 h and 36 h after challenge (P < 0.05), lower bacterial shedding in milk 12 h after challenge (P < 0.01) and a minor decrease in total milk yield 12 h and 24 h after challenge (P < 0.01) compared to q-uniparous cows.
Conclusion: An in vivo infection model to study the impact of genetic selection for mastitis susceptibility under defined conditions in dairy uniparous cows was successfully established and revealed significant differences between the two genetically selected haplotype groups. This result might explain their differences in susceptibility towards IMI. These clinical findings form the basis for further in-depth molecular analysis to clarify the underlying genetic mechanisms for mastitis resistance.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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