Background: There are gender differences in the biotransformation of arsenic. We investigated the effects of gender differences on arsenic metabolism and arsenic toxicity mechanisms in rat liver tissues.
Methods: Rats were treated with different amounts of arsenic compounds. Arsenic form MMA and DMA in the liver was determined by high performance liquid chromatography-hydride generation atomic fluorescence spectroscopy. SAM, ARR, NAD, PNP, PK, and MPO in rat liver were determined by enzyme-linked immunoassay. RT-qPCR was used to determine AS3MT in the liver.
Results: Compared with male and female animals in the same group, MMA and DMA were statistically significant in the three groups of iAs3 + high, iAs3 + medium and iAs5+ low (P <0.05). The MMA of male rats in iAs3+ high and medium groups was higher than that of female rats, and the DMA of male rats was lower than that of female rats. As3MT mRNA in the male iAs3+ high group was higher than that of females. Besides, compared between male and female, only in iAS3+ low dose, iAS3+ medium dose, iAS5+ low dose, and iAS5+ medium dose groups, there was significant difference in SAM level (P<0.05). Compared with male and female animals in the same group, male rats had significantly higher PNP and ARR activities while lower PK activity than female rats (P<0.05). Between the male and female groups, only the iAS3+ high dose and medium dose group had a statistically significant difference (P<0.05). The NAD activity of females in iAS3+ high dose group was higher than that of males.
Conclusion: Conclusively, under the same arsenic exposure, there were gender differences between female and male rats, and arsenic metabolism was more cytotoxic to male rats than to females.
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No competing interests reported.
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Posted 03 Mar, 2021
On 24 Mar, 2021
On 09 Mar, 2021
On 09 Mar, 2021
On 09 Mar, 2021
Received 07 Mar, 2021
Received 07 Mar, 2021
Received 07 Mar, 2021
Received 07 Mar, 2021
Received 07 Mar, 2021
Received 07 Mar, 2021
Received 07 Mar, 2021
On 06 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
Invitations sent on 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 17 Feb, 2021
Posted 03 Mar, 2021
On 24 Mar, 2021
On 09 Mar, 2021
On 09 Mar, 2021
On 09 Mar, 2021
Received 07 Mar, 2021
Received 07 Mar, 2021
Received 07 Mar, 2021
Received 07 Mar, 2021
Received 07 Mar, 2021
Received 07 Mar, 2021
Received 07 Mar, 2021
On 06 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
Invitations sent on 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 02 Mar, 2021
On 17 Feb, 2021
Background: There are gender differences in the biotransformation of arsenic. We investigated the effects of gender differences on arsenic metabolism and arsenic toxicity mechanisms in rat liver tissues.
Methods: Rats were treated with different amounts of arsenic compounds. Arsenic form MMA and DMA in the liver was determined by high performance liquid chromatography-hydride generation atomic fluorescence spectroscopy. SAM, ARR, NAD, PNP, PK, and MPO in rat liver were determined by enzyme-linked immunoassay. RT-qPCR was used to determine AS3MT in the liver.
Results: Compared with male and female animals in the same group, MMA and DMA were statistically significant in the three groups of iAs3 + high, iAs3 + medium and iAs5+ low (P <0.05). The MMA of male rats in iAs3+ high and medium groups was higher than that of female rats, and the DMA of male rats was lower than that of female rats. As3MT mRNA in the male iAs3+ high group was higher than that of females. Besides, compared between male and female, only in iAS3+ low dose, iAS3+ medium dose, iAS5+ low dose, and iAS5+ medium dose groups, there was significant difference in SAM level (P<0.05). Compared with male and female animals in the same group, male rats had significantly higher PNP and ARR activities while lower PK activity than female rats (P<0.05). Between the male and female groups, only the iAS3+ high dose and medium dose group had a statistically significant difference (P<0.05). The NAD activity of females in iAS3+ high dose group was higher than that of males.
Conclusion: Conclusively, under the same arsenic exposure, there were gender differences between female and male rats, and arsenic metabolism was more cytotoxic to male rats than to females.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
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