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Research Article
Jun Wu
Public Health College of Xinjiang Medical University
Corresponding Author
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Background: There are gender differences in the biotransformation of arsenic. We investigated the effects of gender differences on arsenic metabolism and arsenic toxicity mechanisms in rat liver tissues.
Methods: Rats were treated with different amounts of arsenic compounds. Arsenic form MMA and DMA in the liver was determined by high performance liquid chromatography-hydride generation atomic fluorescence spectroscopy. SAM, ARR, NAD, PNP, PK, and MPO in rat liver were determined by enzyme-linked immunoassay. RT-qPCR was used to determine AS3MT in the liver.
Results: Compared with male and female animals in the same group, MMA and DMA were statistically significant in the three groups of iAs3 + high, iAs3 + medium and iAs5+ low (P <0.05). The MMA of male rats in iAs3+ high and medium groups was higher than that of female rats, and the DMA of male rats was lower than that of female rats. As3MT mRNA in the male iAs3+ high group was higher than that of females. Besides, compared between male and female, only in iAS3+ low dose, iAS3+ medium dose, iAS5+ low dose, and iAS5+ medium dose groups, there was significant difference in SAM level (P<0.05). Compared with male and female animals in the same group, male rats had significantly higher PNP and ARR activities while lower PK activity than female rats (P<0.05). Between the male and female groups, only the iAS3+ high dose and medium dose group had a statistically significant difference (P<0.05). The NAD activity of females in iAS3+ high dose group was higher than that of males.
Conclusion: Conclusively, under the same arsenic exposure, there were gender differences between female and male rats, and arsenic metabolism was more cytotoxic to male rats than to females.
Keywords
Arsenic, Gender difference, MMA, DMA, SAM, ARR, NAD, PNP, PK, MPO, As3MT
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No competing interests reported.
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CURRENT STATUS: Under Revision
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Editorial decision: Major revision
On 24 Mar, 2021
Reviewer #3 agreed
On 09 Mar, 2021
Reviewer #2 agreed
On 09 Mar, 2021
Reviewer #4 agreed
On 09 Mar, 2021
Review #6 received
Received 07 Mar, 2021
Review #2 received
Received 07 Mar, 2021
Review #4 received
Received 07 Mar, 2021
Review #7 received
Received 07 Mar, 2021
Review #3 received
Received 07 Mar, 2021
Review #1 received
Received 07 Mar, 2021
Review #5 received
Received 07 Mar, 2021
Reviewer #5 agreed
On 06 Mar, 2021
Reviewer #13 agreed
On 02 Mar, 2021
Reviewer #9 agreed
On 02 Mar, 2021
Reviewer #11 agreed
On 02 Mar, 2021
Reviewer #14 agreed
On 02 Mar, 2021
Reviewer #10 agreed
On 02 Mar, 2021
Reviewer #7 agreed
On 02 Mar, 2021
Reviewer #8 agreed
On 02 Mar, 2021
Reviewer #12 agreed
On 02 Mar, 2021
Reviewer #6 agreed
On 02 Mar, 2021
Reviewer #1 agreed
On 02 Mar, 2021
Reviewers invited
Invitations sent on 02 Mar, 2021
Editor assigned
On 02 Mar, 2021
Editor invited
On 02 Mar, 2021
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On 02 Mar, 2021
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This preprint is under consideration at BMC Pharmacology and Toxicology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Jun Wu
Public Health College of Xinjiang Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 03 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 24 Mar, 2021
Reviewer #3 agreed
On 09 Mar, 2021
Reviewer #2 agreed
On 09 Mar, 2021
Reviewer #4 agreed
On 09 Mar, 2021
Review #6 received
Received 07 Mar, 2021
Review #2 received
Received 07 Mar, 2021
Review #4 received
Received 07 Mar, 2021
Review #7 received
Received 07 Mar, 2021
Review #3 received
Received 07 Mar, 2021
Review #1 received
Received 07 Mar, 2021
Review #5 received
Received 07 Mar, 2021
Reviewer #5 agreed
On 06 Mar, 2021
Reviewer #13 agreed
On 02 Mar, 2021
Reviewer #9 agreed
On 02 Mar, 2021
Reviewer #11 agreed
On 02 Mar, 2021
Reviewer #14 agreed
On 02 Mar, 2021
Reviewer #10 agreed
On 02 Mar, 2021
Reviewer #7 agreed
On 02 Mar, 2021
Reviewer #8 agreed
On 02 Mar, 2021
Reviewer #12 agreed
On 02 Mar, 2021
Reviewer #6 agreed
On 02 Mar, 2021
Reviewer #1 agreed
On 02 Mar, 2021
Reviewers invited
Invitations sent on 02 Mar, 2021
Editor assigned
On 02 Mar, 2021
Editor invited
On 02 Mar, 2021
Submission checks complete
On 02 Mar, 2021
First submitted
On 17 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: There are gender differences in the biotransformation of arsenic. We investigated the effects of gender differences on arsenic metabolism and arsenic toxicity mechanisms in rat liver tissues.
Methods: Rats were treated with different amounts of arsenic compounds. Arsenic form MMA and DMA in the liver was determined by high performance liquid chromatography-hydride generation atomic fluorescence spectroscopy. SAM, ARR, NAD, PNP, PK, and MPO in rat liver were determined by enzyme-linked immunoassay. RT-qPCR was used to determine AS3MT in the liver.
Results: Compared with male and female animals in the same group, MMA and DMA were statistically significant in the three groups of iAs3 + high, iAs3 + medium and iAs5+ low (P <0.05). The MMA of male rats in iAs3+ high and medium groups was higher than that of female rats, and the DMA of male rats was lower than that of female rats. As3MT mRNA in the male iAs3+ high group was higher than that of females. Besides, compared between male and female, only in iAS3+ low dose, iAS3+ medium dose, iAS5+ low dose, and iAS5+ medium dose groups, there was significant difference in SAM level (P<0.05). Compared with male and female animals in the same group, male rats had significantly higher PNP and ARR activities while lower PK activity than female rats (P<0.05). Between the male and female groups, only the iAS3+ high dose and medium dose group had a statistically significant difference (P<0.05). The NAD activity of females in iAS3+ high dose group was higher than that of males.
Conclusion: Conclusively, under the same arsenic exposure, there were gender differences between female and male rats, and arsenic metabolism was more cytotoxic to male rats than to females.
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Figure 5
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