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Research Article
Karl-Heinz Rexer
Philipps-Universität Marburg
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5513-6576
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Morphological, anatomical and ultrastructural characteristics are important for taxonomical and phylogenetic studies of fungi. For scanning electron microscopy, usually only dry voucher specimens are available. For dried plant material, Aerosol OT has been shown to be a suitable rehydration agent for SEM preparation. For swelling and stabilization of fungal cells, however, this simple method does not yield satisfactory results. Here, we show that a combination of Aerosol OT with ultrasonic bath and rehydration in a vacuum desiccator is a good method to distend fungal cells like basidiospores, pleuro- and cheilocystidia for SEM analysis. Tissues of several species of Agaricomycetes with diverse morphological structures were exposed to the treatment. Diverse concentrations of Aerosol OT as well as treatments in an ultrasonic bath and a vacuum desiccator were tested to optimize the surface reconstruction and to reduce preparation artefacts. The evaluated rehydration method is a cheap, quick and nontoxic method to prepare dried specimens of fungal cells for SEM analysis.
Keywords
AOT, Agaricomycetes, dried fungal specimen, rehydration, SEM
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This preprint is under consideration at Mycological Progress. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Karl-Heinz Rexer
Philipps-Universität Marburg
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5513-6576
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 04 Mar, 2021
No community comments so far
Reviews received
Received 25 Feb, 2021
Reviewers invited
Invitations sent on 23 Feb, 2021
First submitted
On 17 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
General Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Morphological, anatomical and ultrastructural characteristics are important for taxonomical and phylogenetic studies of fungi. For scanning electron microscopy, usually only dry voucher specimens are available. For dried plant material, Aerosol OT has been shown to be a suitable rehydration agent for SEM preparation. For swelling and stabilization of fungal cells, however, this simple method does not yield satisfactory results. Here, we show that a combination of Aerosol OT with ultrasonic bath and rehydration in a vacuum desiccator is a good method to distend fungal cells like basidiospores, pleuro- and cheilocystidia for SEM analysis. Tissues of several species of Agaricomycetes with diverse morphological structures were exposed to the treatment. Diverse concentrations of Aerosol OT as well as treatments in an ultrasonic bath and a vacuum desiccator were tested to optimize the surface reconstruction and to reduce preparation artefacts. The evaluated rehydration method is a cheap, quick and nontoxic method to prepare dried specimens of fungal cells for SEM analysis.
Figure 1
Figure 2
Figure 3
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