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Learn more about In Review
Research Article
Zohra Saleem
Dow University of Health Sciences
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background:
Oral squamous cell carcinoma is a global threat and accounts for approximately 90% of malignant oral lesions. The emergence of oral carcinoma is linked to precancerous lesions which act as precursors of the disease. Matrix metalloproteinases appear to play a significant role in the pathogenesis of both precancerous conditions and oral malignancies due to their participation in the remodelling of the extracellular matrix.
Methodology:
This is an analytical study conducted at Dow University of Health Sciences, Karachi, Pakistan. Unstimulated saliva samples were collected from healthy, oral submucous fibrosis and oral squamous cell carcinoma patients. The level of MMP-12 was estimated using enzyme-linked immunosorbent assay (ELISA). One-way analysis of variance (ANOVA) was run to determine if MMP-12 levels differ between cases and controls which was preceded by posthoc Tuckey test.
Results
A significant difference in salivary MMP-12 expression was observed in OSF and OSCC. The expression of salivary MMP-12 was higher in cases compared to controls. The mean MMP-12 expression in OSCC was found higher than in OSF cases.
Conclusion
MMP-12 expression increases as the healthy patient advances to OSF and OSCC. The study results also demonstrate higher MMP-12 expression in OSCC patients as compared to OSF. Therefore, estimation of salivary MMP-12 serves as a useful non-invasive early diagnostic tool in the diagnosis of oral submucous fibrosis and oral squamous cell carcinoma.
Keywords
Matrix metalloproteinase, MMP-12, Oral Squamous cell carcinoma, Oral Submucous fibrosis, Salivary biomarker, ELISA
No competing interests reported.
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Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 03 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 22 Mar, 2021
Review #2 received
Received 19 Mar, 2021
Review #1 received
Received 19 Mar, 2021
Review #3 received
Received 19 Mar, 2021
Review #4 received
Received 19 Mar, 2021
Reviewer #5 agreed
On 11 Mar, 2021
Reviewer #6 agreed
On 08 Mar, 2021
Reviewer #2 agreed
On 08 Mar, 2021
Reviewer #10 agreed
On 08 Mar, 2021
Reviewer #1 agreed
On 08 Mar, 2021
Reviewer #3 agreed
On 08 Mar, 2021
Reviewer #8 agreed
On 08 Mar, 2021
Reviewer #9 agreed
On 08 Mar, 2021
Reviewer #7 agreed
On 08 Mar, 2021
Reviewer #4 agreed
On 08 Mar, 2021
Reviewer #11 agreed
On 08 Mar, 2021
Reviewer #12 agreed
On 08 Mar, 2021
Reviewers invited
Invitations sent on 04 Mar, 2021
Editor assigned
On 26 Feb, 2021
Editor invited
On 22 Feb, 2021
Submission checks complete
On 22 Feb, 2021
First submitted
On 18 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Dentistry
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This preprint is under consideration at BMC Oral Health. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Zohra Saleem
Dow University of Health Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 03 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 22 Mar, 2021
Review #2 received
Received 19 Mar, 2021
Review #1 received
Received 19 Mar, 2021
Review #3 received
Received 19 Mar, 2021
Review #4 received
Received 19 Mar, 2021
Reviewer #5 agreed
On 11 Mar, 2021
Reviewer #6 agreed
On 08 Mar, 2021
Reviewer #2 agreed
On 08 Mar, 2021
Reviewer #10 agreed
On 08 Mar, 2021
Reviewer #1 agreed
On 08 Mar, 2021
Reviewer #3 agreed
On 08 Mar, 2021
Reviewer #8 agreed
On 08 Mar, 2021
Reviewer #9 agreed
On 08 Mar, 2021
Reviewer #7 agreed
On 08 Mar, 2021
Reviewer #4 agreed
On 08 Mar, 2021
Reviewer #11 agreed
On 08 Mar, 2021
Reviewer #12 agreed
On 08 Mar, 2021
Reviewers invited
Invitations sent on 04 Mar, 2021
Editor assigned
On 26 Feb, 2021
Editor invited
On 22 Feb, 2021
Submission checks complete
On 22 Feb, 2021
First submitted
On 18 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Dentistry
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background:
Oral squamous cell carcinoma is a global threat and accounts for approximately 90% of malignant oral lesions. The emergence of oral carcinoma is linked to precancerous lesions which act as precursors of the disease. Matrix metalloproteinases appear to play a significant role in the pathogenesis of both precancerous conditions and oral malignancies due to their participation in the remodelling of the extracellular matrix.
Methodology:
This is an analytical study conducted at Dow University of Health Sciences, Karachi, Pakistan. Unstimulated saliva samples were collected from healthy, oral submucous fibrosis and oral squamous cell carcinoma patients. The level of MMP-12 was estimated using enzyme-linked immunosorbent assay (ELISA). One-way analysis of variance (ANOVA) was run to determine if MMP-12 levels differ between cases and controls which was preceded by posthoc Tuckey test.
Results
A significant difference in salivary MMP-12 expression was observed in OSF and OSCC. The expression of salivary MMP-12 was higher in cases compared to controls. The mean MMP-12 expression in OSCC was found higher than in OSF cases.
Conclusion
MMP-12 expression increases as the healthy patient advances to OSF and OSCC. The study results also demonstrate higher MMP-12 expression in OSCC patients as compared to OSF. Therefore, estimation of salivary MMP-12 serves as a useful non-invasive early diagnostic tool in the diagnosis of oral submucous fibrosis and oral squamous cell carcinoma.
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