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Research article
Transcriptomic and proteomic analysis of putative digestive proteases in the salivary gland and gut of Empoasca (Matsumurasca) onukii Matsuda
Sijun Liu
Iowa State University
Corresponding Author
Zhipeng Huang
Fujian Agriculture and Forestry University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Infestation by tea green leafhoppers, Empoasca (Matsumurasca) onukii , could cause a series of biochemical changes in tea leaves. As a typical cell-rupture feeder, E. onukii secretes proteases while probing with its stylet into the tender shoots of tea plants ( Camellia sinensis ). This study identified and analyzed proteases specifically expressed in the salivary gland (SG) and gut of E. onukii through enzymatic activity assays, complemented with an integrated analysis of transcriptome and proteome data.
Results: In total, 129 contigs representing seven types of putative proteases were identified. Transcript abundance of digestive proteases and enzymatic activity assays showed that cathepsin B-like protease, cathepsin L-like protease, and serine proteases (trypsin- and chymotrypsin-like protease) were highly abundant in the gut while moderately abundant in the SG. The abundance pattern of digestive proteases in the SG and gut of E. onukii differed from that of other hemipterans including Nilaparvata lugens , Laodelphax striatellus , Acyrthosiphum pisum , Halyomorpha halys and Nephotettix cincticeps . Phylogenetic analysis showed that aminopeptidase N-like proteins and serine proteases abundant in the SG or gut of hemipterans were distributed to two distinct clusters.
Conclusions: Altogether, this study provide insightful information on the digestive system of E. onukii and observed different patterns of proteases abundant in the SG and gut of E. onukii , in comparison with other five hemipteran species. These results will be beneficial in understanding the interaction between tea plants and E. onukii .
Keywords
tea green leafhopper, RNA-Seq, proteomics, enzymatic activity, salivary gland, gut
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This is a list of supplementary files associated with this preprint. Click to download.
- Additionalfile1.pdf
File name: Additional file 1.pdf Title of data: Additional tables Description of data: This file contains 2 additional tables.
- Additionalfile2.pdf
File name: Additional file 2.pdf Title of data: Additional Figures Description of data: This file contains 4 additional figures.
- Additionalfile3.xlsx
File name: Additional file 3.xlsx Title of data: Putative proteases in E. onukii Description of data: This file lists information of predicted proteases identified from salivary glands and gut of E. onukii.
- Additionalfile4.txt
File name: Additional file 4.txt Title of data: Protein sequence of putative proteases in E. onukii and N. cincticeps Description of data: This file lists protein sequence of putative proteases in E. onukii and N. cincticeps.
- Additionalfile5.xlsx
File name: Additional file 5.xlsx Title of data: The top 100 abundant proteins in SG or gut of E. onukii Description of data: This file lists information of the top 100 abundant proteins in SG or gut of E. onukii.
- Additionalfile6.xlsx
File name: Additional file 6.xlsx Title of data: Information of insect protease proteins included in the analysis Description of data: This file lists information of protease proteins included in the analysis from other insect species besides of E. onukii.
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Review #1 received
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Review #2 received
Received 26 May, 2020
Reviewer #2 agreed
On 20 May, 2020
Reviewers invited
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Reviewer #1 agreed
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This preprint is under consideration at BMC Genomics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Transcriptomic and proteomic analysis of putative digestive proteases in the salivary gland and gut of Empoasca (Matsumurasca) onukii Matsuda
Sijun Liu
Iowa State University
Corresponding Author
Zhipeng Huang
Fujian Agriculture and Forestry University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 2
Posted 11 Aug, 2020
No community comments so far
Review #2 received
Received 28 Feb, 2021
Reviewer #2 agreed
On 09 Feb, 2021
Review #1 received
Received 07 Sep, 2020
Reviewer #1 agreed
On 10 Aug, 2020
Reviewers invited
Invitations sent on 05 Aug, 2020
Editor assigned
On 02 Aug, 2020
Submission checks complete
On 01 Aug, 2020
Editor invited
On 01 Aug, 2020
No comments provided
Editorial decision: Major revision
On 01 Jul, 2020
Review #1 received
Received 28 Jun, 2020
Review #2 received
Received 26 May, 2020
Reviewer #2 agreed
On 20 May, 2020
Reviewers invited
Invitations sent on 18 May, 2020
Reviewer #1 agreed
On 18 May, 2020
Editor assigned
On 30 Apr, 2020
First submitted
On 29 Apr, 2020
Submission checks complete
On 29 Apr, 2020
Editor invited
On 29 Apr, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Infestation by tea green leafhoppers, Empoasca (Matsumurasca) onukii , could cause a series of biochemical changes in tea leaves. As a typical cell-rupture feeder, E. onukii secretes proteases while probing with its stylet into the tender shoots of tea plants ( Camellia sinensis ). This study identified and analyzed proteases specifically expressed in the salivary gland (SG) and gut of E. onukii through enzymatic activity assays, complemented with an integrated analysis of transcriptome and proteome data.
Results: In total, 129 contigs representing seven types of putative proteases were identified. Transcript abundance of digestive proteases and enzymatic activity assays showed that cathepsin B-like protease, cathepsin L-like protease, and serine proteases (trypsin- and chymotrypsin-like protease) were highly abundant in the gut while moderately abundant in the SG. The abundance pattern of digestive proteases in the SG and gut of E. onukii differed from that of other hemipterans including Nilaparvata lugens , Laodelphax striatellus , Acyrthosiphum pisum , Halyomorpha halys and Nephotettix cincticeps . Phylogenetic analysis showed that aminopeptidase N-like proteins and serine proteases abundant in the SG or gut of hemipterans were distributed to two distinct clusters.
Conclusions: Altogether, this study provide insightful information on the digestive system of E. onukii and observed different patterns of proteases abundant in the SG and gut of E. onukii , in comparison with other five hemipteran species. These results will be beneficial in understanding the interaction between tea plants and E. onukii .
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7