This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Yang Wang
Hainan Medical University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The passive tension force enhancement is one kind of myogenic spontaneous fasciculation in muscles. However, its physiological properties in cardiac fibres are not well known. In this study, mice cardiac papillary muscle spontaneous force enhancement was evaluated by micro stepping stretch method. The occurrence of spontaneous force and real time cardiac fibre Ca2+ redistribution was tranced by Flou-3 (2mM) indicator. Force enhancement amplitude, enhancement prolonging time, and tension–time integral were analysis by myograph analyser. The results indicated that the spontaneous force occurred immediately after the active stretch, rapidly enhanced during tolerating the sustained static stretch. The force occurrence and amplitude enhance synchronized with the Ca2+ recruitment and lightning transmitted to adjacent fibres. In high preload fibres, the enhancement was forceful to over its maximum passive tension (6.20 ± 0.51 N/mm2 to 4.49 ± 0.43 N/mm2). The force occurrences were unsteadiness in each stretch. The increased enhancement amplitude combining with the shortening prolonging time induced reduction of tension–time integral. We concluded that the intracellular Ca2+ synchronized force enhancement is one kind of interruption event in overloading cardiac fibres. This interruption occurred during the relaxation processing in cardiac muscle, therefore affect the rhythmic stability of cardiac relaxation-contraction cycle.
Keywords
cardiac papillary muscle, mechanical stepping stretch, myogenic spontaneous force
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 03 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 13 Apr, 2021
Review #3 received
Received 12 Apr, 2021
Review #4 received
Received 07 Apr, 2021
Review #2 received
Received 07 Apr, 2021
Review #1 received
Received 07 Apr, 2021
Reviewer #1 agreed
On 06 Apr, 2021
Reviewer #4 agreed
On 06 Apr, 2021
Reviewer #3 agreed
On 06 Apr, 2021
Reviewer #9 agreed
On 06 Apr, 2021
Reviewer #2 agreed
On 06 Apr, 2021
Reviewer #5 agreed
On 06 Apr, 2021
Reviewer #7 agreed
On 06 Apr, 2021
Reviewer #6 agreed
On 06 Apr, 2021
Reviewers invited
Invitations sent on 06 Apr, 2021
Editor assigned
On 06 Apr, 2021
Editor invited
On 28 Feb, 2021
Submission checks complete
On 27 Feb, 2021
First submitted
On 18 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cardiac & Cardiovascular Systems
Learn more about our company.
This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Yang Wang
Hainan Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 03 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 13 Apr, 2021
Review #3 received
Received 12 Apr, 2021
Review #4 received
Received 07 Apr, 2021
Review #2 received
Received 07 Apr, 2021
Review #1 received
Received 07 Apr, 2021
Reviewer #1 agreed
On 06 Apr, 2021
Reviewer #4 agreed
On 06 Apr, 2021
Reviewer #3 agreed
On 06 Apr, 2021
Reviewer #9 agreed
On 06 Apr, 2021
Reviewer #2 agreed
On 06 Apr, 2021
Reviewer #5 agreed
On 06 Apr, 2021
Reviewer #7 agreed
On 06 Apr, 2021
Reviewer #6 agreed
On 06 Apr, 2021
Reviewers invited
Invitations sent on 06 Apr, 2021
Editor assigned
On 06 Apr, 2021
Editor invited
On 28 Feb, 2021
Submission checks complete
On 27 Feb, 2021
First submitted
On 18 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cardiac & Cardiovascular Systems
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The passive tension force enhancement is one kind of myogenic spontaneous fasciculation in muscles. However, its physiological properties in cardiac fibres are not well known. In this study, mice cardiac papillary muscle spontaneous force enhancement was evaluated by micro stepping stretch method. The occurrence of spontaneous force and real time cardiac fibre Ca2+ redistribution was tranced by Flou-3 (2mM) indicator. Force enhancement amplitude, enhancement prolonging time, and tension–time integral were analysis by myograph analyser. The results indicated that the spontaneous force occurred immediately after the active stretch, rapidly enhanced during tolerating the sustained static stretch. The force occurrence and amplitude enhance synchronized with the Ca2+ recruitment and lightning transmitted to adjacent fibres. In high preload fibres, the enhancement was forceful to over its maximum passive tension (6.20 ± 0.51 N/mm2 to 4.49 ± 0.43 N/mm2). The force occurrences were unsteadiness in each stretch. The increased enhancement amplitude combining with the shortening prolonging time induced reduction of tension–time integral. We concluded that the intracellular Ca2+ synchronized force enhancement is one kind of interruption event in overloading cardiac fibres. This interruption occurred during the relaxation processing in cardiac muscle, therefore affect the rhythmic stability of cardiac relaxation-contraction cycle.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Loading...