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Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging
Liming Wang
Henan University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9508-8927
Jiaqiang Sun
Institute of Crop Science Chinese Academy of Agricultural Sciences
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomic Data.
Background: Lesion-mimic and premature aging ( lmpa ) mutant lm pa 1 was identified from the ethyl methane sulfonate (EMS) mutant library in the bread wheat variety Keda 527 (KD527) background. To reveal the genetic basis of l mpa 1 mutant, phenotypic observations and analyses of chlorophyll content and photosynthesis were carried out in l mpa 1 , KD527 and their F 1 and F 2 derivatives. Further, bulked segregation analysis (BSA) in combination with a 660K SNP Chip were conducted on the F 2 segregation population of l mpa 1 /Chinese spring(CS) to locate the l mpa 1 gene.
Results: Most agronomic traits of l mpa 1 were similar to those of KD527 before lesion-like spots appeared. Genetic analysis indicated that the F 1 plants from the crossing of l mpa 1 and KD527 exhibited the l mpa phenotype and the F 2 progenies showed a segregation of normal (wild type, WT) and l mpa , with the ratios of l mpa :WT=124:36（χ 2 =1.008<=3.841), indicating that l mpa is a dominant mutation. The combination of BSA and the SNP Chip analysis of CS, l mpa 1 and l mpa 1 /CS F 2 WT pool (50 plants) and l mpa pool (50 plants) showed that polymorphic SNPs were enriched on chromosome 5A, within a region of 30-40 Mb, indicating that the wheat premature aging gene Lmpa1 was probably located on the short arm of chromosome 5A.
Conclusions: EMS-mutagenized mutant lmpa 1 deriving from elite wheat line KD527 conferred lmpa . lmpa phenotype of l mpa 1 mutant is controlled by a single dominant allele designated as Lmpa1 , which affected wheat growth and development and reduced the thousand grain weight ( tgw ) of single plant in wheat. The gene Lmpa1 was tentatively located within the region of 30-40 Mb near to the short arm of chromosome 5A.
Keywords
Wheat, lmpa1, Mutant, Chromosomal location
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CURRENT STATUS: Published
Version 1
Posted 14 May, 2020
Published
On 17 Aug, 2020
No community comments so far
Editorial decision: Minor revision
On 09 Jun, 2020
Review #2 received
Received 09 Jun, 2020
Review #1 received
Received 01 Jun, 2020
Reviewer #2 agreed
On 28 May, 2020
Reviewers invited
Invitations sent on 15 May, 2020
Reviewer #1 agreed
On 15 May, 2020
Editor assigned
On 27 Apr, 2020
First submitted
On 26 Apr, 2020
Submission checks complete
On 26 Apr, 2020
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This preprint is under consideration at BMC Genomic Data. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging
Liming Wang
Henan University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9508-8927
Jiaqiang Sun
Institute of Crop Science Chinese Academy of Agricultural Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 1
Posted 14 May, 2020
Published
On 17 Aug, 2020
No community comments so far
Editorial decision: Minor revision
On 09 Jun, 2020
Review #2 received
Received 09 Jun, 2020
Review #1 received
Received 01 Jun, 2020
Reviewer #2 agreed
On 28 May, 2020
Reviewers invited
Invitations sent on 15 May, 2020
Reviewer #1 agreed
On 15 May, 2020
Editor assigned
On 27 Apr, 2020
First submitted
On 26 Apr, 2020
Submission checks complete
On 26 Apr, 2020
Editor invited
On 26 Apr, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Genomic Data.
Background: Lesion-mimic and premature aging ( lmpa ) mutant lm pa 1 was identified from the ethyl methane sulfonate (EMS) mutant library in the bread wheat variety Keda 527 (KD527) background. To reveal the genetic basis of l mpa 1 mutant, phenotypic observations and analyses of chlorophyll content and photosynthesis were carried out in l mpa 1 , KD527 and their F 1 and F 2 derivatives. Further, bulked segregation analysis (BSA) in combination with a 660K SNP Chip were conducted on the F 2 segregation population of l mpa 1 /Chinese spring(CS) to locate the l mpa 1 gene.
Results: Most agronomic traits of l mpa 1 were similar to those of KD527 before lesion-like spots appeared. Genetic analysis indicated that the F 1 plants from the crossing of l mpa 1 and KD527 exhibited the l mpa phenotype and the F 2 progenies showed a segregation of normal (wild type, WT) and l mpa , with the ratios of l mpa :WT=124:36（χ 2 =1.008<=3.841), indicating that l mpa is a dominant mutation. The combination of BSA and the SNP Chip analysis of CS, l mpa 1 and l mpa 1 /CS F 2 WT pool (50 plants) and l mpa pool (50 plants) showed that polymorphic SNPs were enriched on chromosome 5A, within a region of 30-40 Mb, indicating that the wheat premature aging gene Lmpa1 was probably located on the short arm of chromosome 5A.
Conclusions: EMS-mutagenized mutant lmpa 1 deriving from elite wheat line KD527 conferred lmpa . lmpa phenotype of l mpa 1 mutant is controlled by a single dominant allele designated as Lmpa1 , which affected wheat growth and development and reduced the thousand grain weight ( tgw ) of single plant in wheat. The gene Lmpa1 was tentatively located within the region of 30-40 Mb near to the short arm of chromosome 5A.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the manuscript can be downloaded and accessed as a PDF.