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Original article
Jing Yao
Heilongjiang University of Chinese Medicine
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The present research aimed at evaluating the protective role of betalain on the in vitro glaucoma model using PC12 neuronal cells. The cultured neuronal cells in a customized pressurized chamber were analyzed for the onset of glutathione, myeloperoxidase (MPO), cathepsin, expression of inflammatory enzymes such as cyclooxygenase (COX-1), lipooxygenase (5- LOX), sPLA2 caveolin-1, glaucoma markers and other inflammatory cytokines in the presence and absence of betalain. The results have shown that a significant increase in the expression of oxidative stress with increased activity of cathepsin B and D. On the other hand, the activity of inflammatory enzymes such as COX-1, 5- LOX, sPLA2 were significantly increased in pressure exposed cells. In addition, glaucoma simulated cells demonstrated a significant increase in the VEGF, TGF-β, BDGF, and neuroserpin compared to control. Moreover, cells predisposed to hydrostatic pressure demonstrated an increase in (p<0.01) inflammatory cytokines such as IL-6, CXCR4, IL-17, IL-1β, and TNF-α levels. However, cells pre-treated with betalain improved the glutathione levels with attenuated MPO activity. Simultaneously, the levels of inflammatory cytokines and other glaucoma marker genes found restored in drug pre-treated cells. Thus, the results of the present study demonstrate that the use of betalain on ocular cells can prevent the progression of the disease that can be a suggestive therapeutic for controlling glaucoma like conditions.
Keywords
Betalain, inflammatory, glaucoma, cytokines
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CURRENT STATUS: Published
View the published article at AMB Express.
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Posted 29 Jun, 2020
No community comments so far
Editorial decision: Accept
On 05 Jul, 2020
Review #1 received
Received 04 Jul, 2020
Reviewer #1 agreed
On 03 Jul, 2020
Reviewers invited
Invitations sent on 02 Jul, 2020
Editor assigned
On 27 Jun, 2020
Submission checks complete
On 26 Jun, 2020
Editor invited
On 26 Jun, 2020
No comments provided
Editorial decision: Major Revision
On 07 Jun, 2020
Review #2 received
Received 27 May, 2020
Review #1 received
Received 27 May, 2020
Reviewers invited
Invitations sent on 22 May, 2020
Reviewer #1 agreed
On 22 May, 2020
Reviewer #2 agreed
On 22 May, 2020
Editor assigned
On 06 May, 2020
Editor invited
On 05 May, 2020
Submission checks complete
On 01 May, 2020
First submitted
On 29 Apr, 2020
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See the published version of this preprint at AMB Express.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Original article
Jing Yao
Heilongjiang University of Chinese Medicine
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at AMB Express.
Version 2
Posted 29 Jun, 2020
No community comments so far
Editorial decision: Accept
On 05 Jul, 2020
Review #1 received
Received 04 Jul, 2020
Reviewer #1 agreed
On 03 Jul, 2020
Reviewers invited
Invitations sent on 02 Jul, 2020
Editor assigned
On 27 Jun, 2020
Submission checks complete
On 26 Jun, 2020
Editor invited
On 26 Jun, 2020
No comments provided
Editorial decision: Major Revision
On 07 Jun, 2020
Review #2 received
Received 27 May, 2020
Review #1 received
Received 27 May, 2020
Reviewers invited
Invitations sent on 22 May, 2020
Reviewer #1 agreed
On 22 May, 2020
Reviewer #2 agreed
On 22 May, 2020
Editor assigned
On 06 May, 2020
Editor invited
On 05 May, 2020
Submission checks complete
On 01 May, 2020
First submitted
On 29 Apr, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at AMB Express.
The present research aimed at evaluating the protective role of betalain on the in vitro glaucoma model using PC12 neuronal cells. The cultured neuronal cells in a customized pressurized chamber were analyzed for the onset of glutathione, myeloperoxidase (MPO), cathepsin, expression of inflammatory enzymes such as cyclooxygenase (COX-1), lipooxygenase (5- LOX), sPLA2 caveolin-1, glaucoma markers and other inflammatory cytokines in the presence and absence of betalain. The results have shown that a significant increase in the expression of oxidative stress with increased activity of cathepsin B and D. On the other hand, the activity of inflammatory enzymes such as COX-1, 5- LOX, sPLA2 were significantly increased in pressure exposed cells. In addition, glaucoma simulated cells demonstrated a significant increase in the VEGF, TGF-β, BDGF, and neuroserpin compared to control. Moreover, cells predisposed to hydrostatic pressure demonstrated an increase in (p<0.01) inflammatory cytokines such as IL-6, CXCR4, IL-17, IL-1β, and TNF-α levels. However, cells pre-treated with betalain improved the glutathione levels with attenuated MPO activity. Simultaneously, the levels of inflammatory cytokines and other glaucoma marker genes found restored in drug pre-treated cells. Thus, the results of the present study demonstrate that the use of betalain on ocular cells can prevent the progression of the disease that can be a suggestive therapeutic for controlling glaucoma like conditions.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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