This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
chunhui cui
Southern Medical University
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics.
Methods: In this work, we synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of cancer cells. Seed-mediated synthesized gold nanorods were modified with GX1 peptide-modified amine-termi-nated generation 3 poly(amidoamine) dendrimers via Au-S bond. The obtained GX1 modified dendrimer-stabilized Au NRs (Au [email protected]) are performed as a gene delivery vector to gene (FAM172A) for computed tomography (CT) imaging, thermal imaging, photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells).
Results: We find that Au NR @ PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au [email protected]/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. In addition, animal experiment results confirm that Au [email protected]/FAM172A complexes can achieve tumor thermal imaging, PTT and gene therapy after tail vein injection.
Conclusion: The synthesized Au [email protected] is a potential nanoplatform for tumor imaging and treatment.
Keywords
dendrimers, gold nanorods, gene delivery, photothermal therapy, cancer treatment
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This is a list of supplementary files associated with this preprint. Click to download.
- S1.jpeg
TEM images of Au [email protected]
- S2.png
In vivo thermal images of tumor-bearing mice injected with Au [email protected], Au [email protected] and PBS, upon 808 nm-laser irradiations for different periods of time.
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
chunhui cui
Southern Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
The most recent version of this article is available here.
Version (no preprint)
Posted 15 Sep, 2021
This version was not preprinted
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cancer Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
Background: The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics.
Methods: In this work, we synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of cancer cells. Seed-mediated synthesized gold nanorods were modified with GX1 peptide-modified amine-termi-nated generation 3 poly(amidoamine) dendrimers via Au-S bond. The obtained GX1 modified dendrimer-stabilized Au NRs (Au [email protected]) are performed as a gene delivery vector to gene (FAM172A) for computed tomography (CT) imaging, thermal imaging, photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells).
Results: We find that Au NR @ PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au [email protected]/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. In addition, animal experiment results confirm that Au [email protected]/FAM172A complexes can achieve tumor thermal imaging, PTT and gene therapy after tail vein injection.
Conclusion: The synthesized Au [email protected] is a potential nanoplatform for tumor imaging and treatment.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
This is a list of supplementary files associated with this preprint. Click to download.
- S1.jpeg
TEM images of Au [email protected]
- S2.png
In vivo thermal images of tumor-bearing mice injected with Au [email protected], Au [email protected] and PBS, upon 808 nm-laser irradiations for different periods of time.
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