Background: The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics.
Methods: In this work, we synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of cancer cells. Seed-mediated synthesized gold nanorods were modified with GX1 peptide-modified amine-termi-nated generation 3 poly(amidoamine) dendrimers via Au-S bond. The obtained GX1 modified dendrimer-stabilized Au NRs (Au [email protected]) are performed as a gene delivery vector to gene (FAM172A) for computed tomography (CT) imaging, thermal imaging, photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells).
Results: We find that Au NR @ PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au [email protected]/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. In addition, animal experiment results confirm that Au [email protected]/FAM172A complexes can achieve tumor thermal imaging, PTT and gene therapy after tail vein injection.
Conclusion: The synthesized Au [email protected] is a potential nanoplatform for tumor imaging and treatment.
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This is a list of supplementary files associated with this preprint. Click to download.
TEM images of Au [email protected]
In vivo thermal images of tumor-bearing mice injected with Au [email protected], Au [email protected] and PBS, upon 808 nm-laser irradiations for different periods of time.
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Posted 16 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
Received 26 Mar, 2021
Received 17 Mar, 2021
On 17 Mar, 2021
Invitations sent on 11 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 21 Feb, 2021
Posted 16 Mar, 2021
On 30 Mar, 2021
On 30 Mar, 2021
Received 26 Mar, 2021
Received 17 Mar, 2021
On 17 Mar, 2021
Invitations sent on 11 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 10 Mar, 2021
On 21 Feb, 2021
Background: The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics.
Methods: In this work, we synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of cancer cells. Seed-mediated synthesized gold nanorods were modified with GX1 peptide-modified amine-termi-nated generation 3 poly(amidoamine) dendrimers via Au-S bond. The obtained GX1 modified dendrimer-stabilized Au NRs (Au [email protected]) are performed as a gene delivery vector to gene (FAM172A) for computed tomography (CT) imaging, thermal imaging, photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells).
Results: We find that Au NR @ PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au [email protected]/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. In addition, animal experiment results confirm that Au [email protected]/FAM172A complexes can achieve tumor thermal imaging, PTT and gene therapy after tail vein injection.
Conclusion: The synthesized Au [email protected] is a potential nanoplatform for tumor imaging and treatment.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
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