This preprint is under consideration at Journal of Experimental & Clinical Cancer Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
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Research Article
chunhui cui
Southern Medical University
Corresponding Author
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Background: The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics.
Methods: In this work, we synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of cancer cells. Seed-mediated synthesized gold nanorods were modified with GX1 peptide-modified amine-termi-nated generation 3 poly(amidoamine) dendrimers via Au-S bond. The obtained GX1 modified dendrimer-stabilized Au NRs (Au [email protected]) are performed as a gene delivery vector to gene (FAM172A) for computed tomography (CT) imaging, thermal imaging, photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells).
Results: We find that Au NR @ PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au [email protected]/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. In addition, animal experiment results confirm that Au [email protected]/FAM172A complexes can achieve tumor thermal imaging, PTT and gene therapy after tail vein injection.
Conclusion: The synthesized Au [email protected] is a potential nanoplatform for tumor imaging and treatment.
Keywords
dendrimers, gold nanorods, gene delivery, photothermal therapy, cancer treatment
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This is a list of supplementary files associated with this preprint. Click to download.
- S1.jpeg
TEM images of Au [email protected]
- S2.png
In vivo thermal images of tumor-bearing mice injected with Au [email protected], Au [email protected] and PBS, upon 808 nm-laser irradiations for different periods of time.
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CURRENT STATUS: Under Review
Version 1
Posted 16 Mar, 2021
No community comments so far
Reviewer #3 agreed
On 30 Mar, 2021
Reviewer #2 agreed
On 30 Mar, 2021
Review #1 received
Received 26 Mar, 2021
Review #? received
Received 17 Mar, 2021
Reviewer #1 agreed
On 17 Mar, 2021
Reviewers invited
Invitations sent on 11 Mar, 2021
Editor assigned
On 10 Mar, 2021
Submission checks complete
On 10 Mar, 2021
Editor invited
On 10 Mar, 2021
First submitted
On 21 Feb, 2021
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Subject Areas
Cancer Biology
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This preprint is under consideration at Journal of Experimental & Clinical Cancer Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
chunhui cui
Southern Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 16 Mar, 2021
No community comments so far
Reviewer #3 agreed
On 30 Mar, 2021
Reviewer #2 agreed
On 30 Mar, 2021
Review #1 received
Received 26 Mar, 2021
Review #? received
Received 17 Mar, 2021
Reviewer #1 agreed
On 17 Mar, 2021
Reviewers invited
Invitations sent on 11 Mar, 2021
Editor assigned
On 10 Mar, 2021
Submission checks complete
On 10 Mar, 2021
Editor invited
On 10 Mar, 2021
First submitted
On 21 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cancer Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: The exploitation of novel nanomaterials combining diagnostic and therapeutic functionalities within one single nanoplatform is challenging for tumor theranostics.
Methods: In this work, we synthesized dendrimer-modified gold nanorods for combinational gene therapy and photothermal therapy (PTT) of cancer cells. Seed-mediated synthesized gold nanorods were modified with GX1 peptide-modified amine-termi-nated generation 3 poly(amidoamine) dendrimers via Au-S bond. The obtained GX1 modified dendrimer-stabilized Au NRs (Au [email protected]) are performed as a gene delivery vector to gene (FAM172A) for computed tomography (CT) imaging, thermal imaging, photothermal therapy (PTT) and gene therapy of Colon cancer cells (HCT-8 cells).
Results: We find that Au NR @ PAMAM-GX1 can specifically deliver FAM172A to cancer cells with excellent transfection efficiency. The HCT-8 cells treated with the Au [email protected]/FAM172A under laser irradiation have a viability of 20.45%, which is much lower than the survival rate of other single-mode PTT treatment or single-mode gene therapy. In addition, animal experiment results confirm that Au [email protected]/FAM172A complexes can achieve tumor thermal imaging, PTT and gene therapy after tail vein injection.
Conclusion: The synthesized Au [email protected] is a potential nanoplatform for tumor imaging and treatment.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
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