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Unrevealing model compounds of soil conditioners impacts on the wheat straw autohydrolysis efficiency and enzymatic hydrolysis
Qiang Yong
Nanjing Forestry University
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Biotechnology for Biofuels.
Background: Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosics autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that soluble salts, and organic matter plays in this system. Herein, sodium phosphate and sodium humate were employed as model buffering compounds representing soluble salts and organic matter and dosed into a de-ashed wheat straw (DWS) autohydrolysis process to show the potential impacts of WS attached soil conditioners on the WS autohydrolysis efficiency which would further affect the enzymatic digestibility of autohydrolyzed WS.
Results: Results showed that with the increasing loadings of sodium phosphate and sodium humate resulted in elevated pH values (from 4.0 to 5.1 and from 4.1 to 4.7, respectively). Meanwhile, the reductions of xylan removal yields from ~84.3-61.4% to 72.3-53.0% by loading (1~30 g/L) sodium phosphate and sodium humate during WS autohydrolysis lead to a significant decrease of cellulose accessibilities which finally lead to a reduction of the enzymatic digestibility of autohydrolyzed WS from ~75.4-77.2% to 47.3-57.7%.
Conclusion: The existence of different types soil conditioner model compounds result in various component fractions from autohydrolyzed WS in the process of autohydrolysis. A lack of sufficient xylan removal was found to drive the significant decrease in enzymatic accessibility. The results demonstrated the various effects of two typical tested soil conditioners on WS autohydrolysis and enzymatic hydrolysis.
Keywords
Autohydrolysis, Phosphate, Humate, Enzymatic hydrolysis, Enzymatic accessibility
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CURRENT STATUS: Published
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On 13 Jul, 2020
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Editorial decision: Accept
On 05 Jul, 2020
Reviewer #2 agreed
On 20 Jun, 2020
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Received 20 Jun, 2020
Editor assigned
On 19 Jun, 2020
Reviewers invited
Invitations sent on 19 Jun, 2020
Reviewer #1 agreed
On 19 Jun, 2020
Review #1 received
Received 19 Jun, 2020
Submission checks complete
On 18 Jun, 2020
Editor invited
On 18 Jun, 2020
No comments provided
Editorial decision: Major revision
On 07 Jun, 2020
Review #2 received
Received 06 Jun, 2020
Review #3 received
Received 01 Jun, 2020
Reviewer #3 agreed
On 29 May, 2020
Review #1 received
Received 28 May, 2020
Reviewer #2 agreed
On 23 May, 2020
Reviewers invited
Invitations sent on 19 May, 2020
Reviewer #1 agreed
On 19 May, 2020
First submitted
On 02 May, 2020
Editor assigned
On 02 May, 2020
Submission checks complete
On 01 May, 2020
Editor invited
On 01 May, 2020
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Subject Areas
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This preprint is under consideration at Biotechnology for Biofuels. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Unrevealing model compounds of soil conditioners impacts on the wheat straw autohydrolysis efficiency and enzymatic hydrolysis
Qiang Yong
Nanjing Forestry University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 2
Posted 19 Jun, 2020
Published
On 13 Jul, 2020
No community comments so far
Editorial decision: Accept
On 05 Jul, 2020
Reviewer #2 agreed
On 20 Jun, 2020
Review #2 received
Received 20 Jun, 2020
Editor assigned
On 19 Jun, 2020
Reviewers invited
Invitations sent on 19 Jun, 2020
Reviewer #1 agreed
On 19 Jun, 2020
Review #1 received
Received 19 Jun, 2020
Submission checks complete
On 18 Jun, 2020
Editor invited
On 18 Jun, 2020
No comments provided
Editorial decision: Major revision
On 07 Jun, 2020
Review #2 received
Received 06 Jun, 2020
Review #3 received
Received 01 Jun, 2020
Reviewer #3 agreed
On 29 May, 2020
Review #1 received
Received 28 May, 2020
Reviewer #2 agreed
On 23 May, 2020
Reviewers invited
Invitations sent on 19 May, 2020
Reviewer #1 agreed
On 19 May, 2020
First submitted
On 02 May, 2020
Editor assigned
On 02 May, 2020
Submission checks complete
On 01 May, 2020
Editor invited
On 01 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biotechnology and Bioengineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at Biotechnology for Biofuels.
Background: Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosics autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that soluble salts, and organic matter plays in this system. Herein, sodium phosphate and sodium humate were employed as model buffering compounds representing soluble salts and organic matter and dosed into a de-ashed wheat straw (DWS) autohydrolysis process to show the potential impacts of WS attached soil conditioners on the WS autohydrolysis efficiency which would further affect the enzymatic digestibility of autohydrolyzed WS.
Results: Results showed that with the increasing loadings of sodium phosphate and sodium humate resulted in elevated pH values (from 4.0 to 5.1 and from 4.1 to 4.7, respectively). Meanwhile, the reductions of xylan removal yields from ~84.3-61.4% to 72.3-53.0% by loading (1~30 g/L) sodium phosphate and sodium humate during WS autohydrolysis lead to a significant decrease of cellulose accessibilities which finally lead to a reduction of the enzymatic digestibility of autohydrolyzed WS from ~75.4-77.2% to 47.3-57.7%.
Conclusion: The existence of different types soil conditioner model compounds result in various component fractions from autohydrolyzed WS in the process of autohydrolysis. A lack of sufficient xylan removal was found to drive the significant decrease in enzymatic accessibility. The results demonstrated the various effects of two typical tested soil conditioners on WS autohydrolysis and enzymatic hydrolysis.
Figure 1
Figure 2
Figure 3
Figure 4