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Research Article
Huanhua Chen
Maternal and Child Health Hospital of Guangxi
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8463-7452
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Azoospermic patients have benefited from both epididymal and testicular spermatozoa intracytoplasmic sperm injection (ICSI) treatment and lasers have been used to identify viable but immotile spermatozoa before the procedure. However, there are limited studies on the safety of laser-assisted selection of immotile spermatozoa. The aim of this study was to investigate the impact of laser-assisted selection of immotile spermatozoa on the obstetric and neonatal outcomes after ICSI.
Methods
A retrospective comparative study was conducted on patients who underwent ICSI treatment with testicular spermatozoa in our Reproductive Medicine Unit from June 2014 to June 2018. The 132 cycles were divided into two groups according to whether laser-assisted selection of spermatozoa was used.
Results
Compared with control group, no significant differences were found in the pregnancy, implantation, miscarriage and live birth rates in the laser group in either fresh or frozen transfer cycles. The cumulative live birth rate in the laser group was 69.70%, which was slightly higher than in the control group (60.61%), but this was not statistically different. There were no differences in the average gestational age, premature birth rate, neonatal birth weight and the malformation rate between the laser and control groups (P > 0.05). In addition, the obstetric outcome between the two groups were not different (P > 0.05).
Conclusions
No negative effect on perinatal and neonatal outcomes was seen by using laser-assisted selection of immotile spermatozoa for TESA-ICSI. This study endorses the use of laser-assisted selection of viable spermatozoa for ICSI cycles.
Keywords
Laser, immotile spermatozoa, testicular sperm aspiration, intracytoplasmic spermatozoa injection, obstetric outcome
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CURRENT STATUS: Under Review
Version 1
Posted 26 Feb, 2021
No community comments so far
Editorial decision: Major revision
On 23 Jun, 2021
Review #2 received
Received 22 Jun, 2021
Review #1 received
Received 14 Jun, 2021
Reviewer #2 agreed
On 09 Jun, 2021
Reviews received
Received 22 Feb, 2021
Reviewer #1 agreed
On 22 Feb, 2021
Reviewers invited
Invitations sent on 22 Feb, 2021
Editor assigned
On 20 Feb, 2021
Submission checks complete
On 20 Feb, 2021
Editor invited
On 20 Feb, 2021
First submitted
On 18 Feb, 2021
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Subject Areas
Endocrinology & Metabolism
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This preprint is under consideration at Reproductive Biology and Endocrinology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Huanhua Chen
Maternal and Child Health Hospital of Guangxi
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8463-7452
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 26 Feb, 2021
No community comments so far
Editorial decision: Major revision
On 23 Jun, 2021
Review #2 received
Received 22 Jun, 2021
Review #1 received
Received 14 Jun, 2021
Reviewer #2 agreed
On 09 Jun, 2021
Reviews received
Received 22 Feb, 2021
Reviewer #1 agreed
On 22 Feb, 2021
Reviewers invited
Invitations sent on 22 Feb, 2021
Editor assigned
On 20 Feb, 2021
Submission checks complete
On 20 Feb, 2021
Editor invited
On 20 Feb, 2021
First submitted
On 18 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Endocrinology & Metabolism
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Azoospermic patients have benefited from both epididymal and testicular spermatozoa intracytoplasmic sperm injection (ICSI) treatment and lasers have been used to identify viable but immotile spermatozoa before the procedure. However, there are limited studies on the safety of laser-assisted selection of immotile spermatozoa. The aim of this study was to investigate the impact of laser-assisted selection of immotile spermatozoa on the obstetric and neonatal outcomes after ICSI.
Methods
A retrospective comparative study was conducted on patients who underwent ICSI treatment with testicular spermatozoa in our Reproductive Medicine Unit from June 2014 to June 2018. The 132 cycles were divided into two groups according to whether laser-assisted selection of spermatozoa was used.
Results
Compared with control group, no significant differences were found in the pregnancy, implantation, miscarriage and live birth rates in the laser group in either fresh or frozen transfer cycles. The cumulative live birth rate in the laser group was 69.70%, which was slightly higher than in the control group (60.61%), but this was not statistically different. There were no differences in the average gestational age, premature birth rate, neonatal birth weight and the malformation rate between the laser and control groups (P > 0.05). In addition, the obstetric outcome between the two groups were not different (P > 0.05).
Conclusions
No negative effect on perinatal and neonatal outcomes was seen by using laser-assisted selection of immotile spermatozoa for TESA-ICSI. This study endorses the use of laser-assisted selection of viable spermatozoa for ICSI cycles.
Figure 1
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