This preprint is under consideration at Plant Cell, Tissue and Organ Culture (PCTOC). A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
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Research Article
Marzieh Taghizadeh
University of Isfahan
Corresponding Author
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Dracocephlum kotschyi Boiss is a genus in Lamiaceae family and a medicinal herb native to Iran. The cell suspension cultures were treated by static magnetic field (SMF) and Fe3O4 magnetite nanoparticles (MNP) to understand the production yield of secondary metabolites. The treatment procedure was done by cultivating the cells either with 100 ppm MNP, SMFs, or simultaneous exposure to both MNP and SMFs. The SMF at 30 mT was uniformly applied to the cells either for 3 or 4 days with a 3 h per day or a 5 h per day intervals, respectively. The contents of phenolics and phytochemicals were then examined by high performance liquid chromatography and UV-Vis spectrophotometer. These treatments imposed oxidative stress and induced polyphenol oxidase and phenylalanine ammonia lyase, accompanied by enhanced production of phenolics, anthocyanins, flavonoids, and lignin. The highest membrane embrittlement and elicitation was found upon simultaneous application of the MNPs and SMFs, followed by the MNP and SMFs. The contents of naringin, rosmarinic acid, quercetin, thymol, carvacrol, apigenin, and rutin increased in the intracellular biomass of all treated cells and extracellular culture media. These findings propose the potential of these elicitors in simultaneous production and secretion of these phytochemicals into culture media.
Keywords
Antioxidant activity, magnetite nanoparticles, secondary metabolites, suspension culture, static magnetic field
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This preprint is under consideration at Plant Cell, Tissue and Organ Culture (PCTOC). A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Marzieh Taghizadeh
University of Isfahan
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 02 Mar, 2021
No community comments so far
Reviews received
Received 10 Mar, 2021
Reviewers invited
Invitations sent on 24 Feb, 2021
Editor assigned
On 22 Feb, 2021
First submitted
On 21 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Dracocephlum kotschyi Boiss is a genus in Lamiaceae family and a medicinal herb native to Iran. The cell suspension cultures were treated by static magnetic field (SMF) and Fe3O4 magnetite nanoparticles (MNP) to understand the production yield of secondary metabolites. The treatment procedure was done by cultivating the cells either with 100 ppm MNP, SMFs, or simultaneous exposure to both MNP and SMFs. The SMF at 30 mT was uniformly applied to the cells either for 3 or 4 days with a 3 h per day or a 5 h per day intervals, respectively. The contents of phenolics and phytochemicals were then examined by high performance liquid chromatography and UV-Vis spectrophotometer. These treatments imposed oxidative stress and induced polyphenol oxidase and phenylalanine ammonia lyase, accompanied by enhanced production of phenolics, anthocyanins, flavonoids, and lignin. The highest membrane embrittlement and elicitation was found upon simultaneous application of the MNPs and SMFs, followed by the MNP and SMFs. The contents of naringin, rosmarinic acid, quercetin, thymol, carvacrol, apigenin, and rutin increased in the intracellular biomass of all treated cells and extracellular culture media. These findings propose the potential of these elicitors in simultaneous production and secretion of these phytochemicals into culture media.
Figure 1
Figure 2
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