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Research article
Molecular genetic diversity and differentiation of Nile tilapia (Oreochromis niloticus, L. 1758) in East African natural and stocked populations
Papius Dias Tibihika
Universitat fur Bodenkultur Wien
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6918-9903
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Evolutionary Biology.
Background The need for enhancing the productivity of fisheries in Africa triggered the introduction of non-native fish, causing dramatic changes to local species. In East Africa, the extensive translocation of Nile tilapia (Oreochromis niloticus) is one of the major factors in this respect. Using 40 microsatellite loci with SSR-GBS techniques, we amplified a total of 664 individuals to investigate the genetic structure of O. niloticus from East Africa in comparison to Ethiopian and Burkina Faso populations. Results All three African regions were characterized by independent gene-pools, however, the Ethiopian population from lake Tana showed to be more divergent than expected suggesting that it might be a different species. In East Africa, the genetic structure was congruent with both geographical location and anthropogenic activities. O. niloticus from Lake Turkana (Kenya) was isolated, while in Uganda, despite populations being rather similar to each other, two main natural catchments were able to be defined. We show that these two groups contributed to the gene-pool of different non-native populations. Moreover, admixture and possible hybridization with other tilapiine species may have contributed to the genetic divergence found in some populations such as Lake Victoria. We detected other factors that might be affecting Nile tilapia genetic variation. For example, most of the populations have gone through a reduction of genetic diversity, which can be a consequence of bottleneck caused by overfishing, genetic erosion due to fragmentation or founder effect resulting from stoking activities. Conclusions The anthropogenic activities particularly in the East African O. niloticus translocations, promoted admixture and contact with the native congenerics which may contribute to outbreeding depression and hence compromising the sustainability of the species in the region.
Keywords
cichlids, fish translocations, genetic structure, gene flow, bottleneck
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Folorunso seun
commented on 29 November, 2020
Great work. Please I also want to do similar work on macrobrachium vollenhovenii along osun River and Lagos lagoon in Nigeria, can you assist in training on the methodology. Thank you.
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CURRENT STATUS: Published
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Posted 03 Jan, 2020
Published
On 30 Jan, 2020
Community comments: 1
Editorial decision: Accept
On 16 Jan, 2020
Editor assigned
On 18 Dec, 2019
Submission checks complete
On 17 Dec, 2019
Editor invited
On 17 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 17 Dec, 2019
Editor assigned
On 28 Nov, 2019
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On 27 Nov, 2019
Editor invited
On 27 Nov, 2019
No comments provided
Editorial decision: Minor revision
On 08 Nov, 2019
Review #2 received
Received 26 Oct, 2019
Reviewer #2 agreed
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Review #1 received
Received 21 Oct, 2019
Reviewer #1 agreed
On 08 Oct, 2019
Reviewers invited
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Editor assigned
On 03 Oct, 2019
Submission checks complete
On 02 Oct, 2019
Editor invited
On 02 Oct, 2019
No comments provided
Editorial decision: Minor revision
On 01 Sep, 2019
Review #1 received
Received 29 Aug, 2019
Review #2 received
Received 19 Aug, 2019
Review #3 received
Received 19 Aug, 2019
Reviewer #2 agreed
On 02 Aug, 2019
Reviewer #3 agreed
On 02 Aug, 2019
Reviewers invited
Invitations sent on 28 Jul, 2019
Reviewer #1 agreed
On 28 Jul, 2019
Submission checks complete
On 19 Jul, 2019
Editor assigned
On 05 Jul, 2019
Editor invited
On 04 Jul, 2019
First submitted
On 03 Jul, 2019
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This preprint is under consideration at BMC Evolutionary Biology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Molecular genetic diversity and differentiation of Nile tilapia (Oreochromis niloticus, L. 1758) in East African natural and stocked populations
Papius Dias Tibihika
Universitat fur Bodenkultur Wien
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6918-9903
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Version 4
Posted 03 Jan, 2020
Published
On 30 Jan, 2020
Community comments: 1
Editorial decision: Accept
On 16 Jan, 2020
Editor assigned
On 18 Dec, 2019
Submission checks complete
On 17 Dec, 2019
Editor invited
On 17 Dec, 2019
No comments provided
Editorial decision: Minor revision
On 17 Dec, 2019
Editor assigned
On 28 Nov, 2019
Submission checks complete
On 27 Nov, 2019
Editor invited
On 27 Nov, 2019
No comments provided
Editorial decision: Minor revision
On 08 Nov, 2019
Review #2 received
Received 26 Oct, 2019
Reviewer #2 agreed
On 21 Oct, 2019
Review #1 received
Received 21 Oct, 2019
Reviewer #1 agreed
On 08 Oct, 2019
Reviewers invited
Invitations sent on 07 Oct, 2019
Editor assigned
On 03 Oct, 2019
Submission checks complete
On 02 Oct, 2019
Editor invited
On 02 Oct, 2019
No comments provided
Editorial decision: Minor revision
On 01 Sep, 2019
Review #1 received
Received 29 Aug, 2019
Review #2 received
Received 19 Aug, 2019
Review #3 received
Received 19 Aug, 2019
Reviewer #2 agreed
On 02 Aug, 2019
Reviewer #3 agreed
On 02 Aug, 2019
Reviewers invited
Invitations sent on 28 Jul, 2019
Reviewer #1 agreed
On 28 Jul, 2019
Submission checks complete
On 19 Jul, 2019
Editor assigned
On 05 Jul, 2019
Editor invited
On 04 Jul, 2019
First submitted
On 03 Jul, 2019
Metrics
Comments: 1
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at BMC Evolutionary Biology.
Background The need for enhancing the productivity of fisheries in Africa triggered the introduction of non-native fish, causing dramatic changes to local species. In East Africa, the extensive translocation of Nile tilapia (Oreochromis niloticus) is one of the major factors in this respect. Using 40 microsatellite loci with SSR-GBS techniques, we amplified a total of 664 individuals to investigate the genetic structure of O. niloticus from East Africa in comparison to Ethiopian and Burkina Faso populations. Results All three African regions were characterized by independent gene-pools, however, the Ethiopian population from lake Tana showed to be more divergent than expected suggesting that it might be a different species. In East Africa, the genetic structure was congruent with both geographical location and anthropogenic activities. O. niloticus from Lake Turkana (Kenya) was isolated, while in Uganda, despite populations being rather similar to each other, two main natural catchments were able to be defined. We show that these two groups contributed to the gene-pool of different non-native populations. Moreover, admixture and possible hybridization with other tilapiine species may have contributed to the genetic divergence found in some populations such as Lake Victoria. We detected other factors that might be affecting Nile tilapia genetic variation. For example, most of the populations have gone through a reduction of genetic diversity, which can be a consequence of bottleneck caused by overfishing, genetic erosion due to fragmentation or founder effect resulting from stoking activities. Conclusions The anthropogenic activities particularly in the East African O. niloticus translocations, promoted admixture and contact with the native congenerics which may contribute to outbreeding depression and hence compromising the sustainability of the species in the region.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Folorunso seun
commented on 29 November, 2020
Great work. Please I also want to do similar work on macrobrachium vollenhovenii along osun River and Lagos lagoon in Nigeria, can you assist in training on the methodology. Thank you.