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Research article
Isolation, identification and 16S rRNA gene analysis of Yersinia enterocolitica strains isolated from the Gymnocypris Przewalskii
Hongjian Zhang
Qinghai University
Corresponding Author
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Background: Yersinia enterocolitica is a human-animal-fish-associated infectious diarrhea pathogen that has caused widespread international attention in recent years. Many strains of Yersinia enterocolitica were identified from different animal species, but there is no information reported Yersinia enterocolitica in Gymnocypris przewalskii . The Gymnocypris przewalskii is a very important species in the Qinghai Lake. They were listed in the China' s second-class protected animal species. Preliminary research on the distribution of Yersinia enterocolitica and the drug sensitive fauna test on Gymnocypris przewalskii was a urgent solving problem for which maintain the original ecological symbiotic system and restore Gymnocypris przewalskii resource. In order to solve these issues, we performed this research.
Results: The results showed that 5 strains Yersinia enterocolitica were obtained, positive ratio was 6.67% (5/75). The average drug-resistant of 5 strain Yersinia enterocolitica was 54.29% (38/70) to 14 kinds of antibiotic. The result of 16S rRNA gene of Yersinia enterocolitica identified showed that one piece of 1419 bp specific braid was obtained. The homologies of nucleotide of 16S rRNA were 91%-95% between 15 strains of Yersinia enterocolitica from GenBank by measuring sequence.
Conclusion: Yersinia enterocolitica can infect Gymnocypris przewalskii . Some strains have drug-resistant effect. The 16S rRNA gene matches 91%-95% with other strains nucleotides download from GenBank and forms a unique branch separated from them.
Keywords
fish, Yersinia enterocolitica, isolation, drug sensitive fauna test
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This is a preprint. It has not completed peer review.
Research article
Isolation, identification and 16S rRNA gene analysis of Yersinia enterocolitica strains isolated from the Gymnocypris Przewalskii
Hongjian Zhang
Qinghai University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 02 Jun, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Yersinia enterocolitica is a human-animal-fish-associated infectious diarrhea pathogen that has caused widespread international attention in recent years. Many strains of Yersinia enterocolitica were identified from different animal species, but there is no information reported Yersinia enterocolitica in Gymnocypris przewalskii . The Gymnocypris przewalskii is a very important species in the Qinghai Lake. They were listed in the China' s second-class protected animal species. Preliminary research on the distribution of Yersinia enterocolitica and the drug sensitive fauna test on Gymnocypris przewalskii was a urgent solving problem for which maintain the original ecological symbiotic system and restore Gymnocypris przewalskii resource. In order to solve these issues, we performed this research.
Results: The results showed that 5 strains Yersinia enterocolitica were obtained, positive ratio was 6.67% (5/75). The average drug-resistant of 5 strain Yersinia enterocolitica was 54.29% (38/70) to 14 kinds of antibiotic. The result of 16S rRNA gene of Yersinia enterocolitica identified showed that one piece of 1419 bp specific braid was obtained. The homologies of nucleotide of 16S rRNA were 91%-95% between 15 strains of Yersinia enterocolitica from GenBank by measuring sequence.
Conclusion: Yersinia enterocolitica can infect Gymnocypris przewalskii . Some strains have drug-resistant effect. The 16S rRNA gene matches 91%-95% with other strains nucleotides download from GenBank and forms a unique branch separated from them.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5