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Research Article
Rita Queiroga
Federal University of Paraíba
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
This study tested the hypothesis that naturally and industrially produced trans fatty acids (TFA) exert distinct effects on rats’ metabolic parameters. Wistar rat groups were fed the diets: CONT - without added fat; HVF − 20% hydrogenated vegetable fat (HVF); and RUM − 20% ruminant fat. Biochemical markers, cytokine levels, fatty acid (FA) composition of liver, heart and adipose tissue, liver histology and the gut microbial composition were also evaluated. Rats fed RUM presented lower deposition of adipose tissue and LDL and higher HDL and anti-inflammatory IL-10 than those fed CONT and HVF. Pro-inflammatory cytokines, malondialdehyde (MDA) and aspartate aminotransferase (AST) were higher in group HVF than CONT and RUM. In all types of tissue, the highest TFA levels were observed in rats fed HVF, which contributed to the development of fatty liver. The RUM diet led to higher concentrations of stearic acid and conjugated linoleic acid (CLA). The lower incorporation of TFA and distinct FA profile in the liver of rats fed RUM compared to HVF could be associated with protection hepatic. The microbial gut communities were significantly dissimilar among the groups. It is plausible that ruminant fat might reduce risk parameters involved in the development of cardiovascular disease.
Keywords
Trans-fatty acids, Conjugated linoleic acid, Hydrogenated Vegetable Fat, Inflammation, Cardiovascular disease,
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No competing interests reported.
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On 13 Mar, 2021
Reviewer #11 agreed
On 13 Mar, 2021
Reviewer #10 agreed
On 13 Mar, 2021
Reviewer #3 agreed
On 13 Mar, 2021
Reviewer #9 agreed
On 13 Mar, 2021
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Rita Queiroga
Federal University of Paraíba
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 04 Mar, 2021
No community comments so far
Review #1 received
Received 21 Mar, 2021
Review #7 received
Received 21 Mar, 2021
Review #10 received
Received 21 Mar, 2021
Review #9 received
Received 21 Mar, 2021
Review #2 received
Received 21 Mar, 2021
Review #8 received
Received 21 Mar, 2021
Reviewer #2 agreed
On 13 Mar, 2021
Reviewer #1 agreed
On 13 Mar, 2021
Reviewer #8 agreed
On 13 Mar, 2021
Reviewer #11 agreed
On 13 Mar, 2021
Reviewer #10 agreed
On 13 Mar, 2021
Reviewer #3 agreed
On 13 Mar, 2021
Reviewer #9 agreed
On 13 Mar, 2021
Reviewers invited
Invitations sent on 08 Mar, 2021
Editor assigned
On 08 Mar, 2021
Editor invited
On 02 Mar, 2021
Submission checks complete
On 01 Mar, 2021
First submitted
On 22 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This study tested the hypothesis that naturally and industrially produced trans fatty acids (TFA) exert distinct effects on rats’ metabolic parameters. Wistar rat groups were fed the diets: CONT - without added fat; HVF − 20% hydrogenated vegetable fat (HVF); and RUM − 20% ruminant fat. Biochemical markers, cytokine levels, fatty acid (FA) composition of liver, heart and adipose tissue, liver histology and the gut microbial composition were also evaluated. Rats fed RUM presented lower deposition of adipose tissue and LDL and higher HDL and anti-inflammatory IL-10 than those fed CONT and HVF. Pro-inflammatory cytokines, malondialdehyde (MDA) and aspartate aminotransferase (AST) were higher in group HVF than CONT and RUM. In all types of tissue, the highest TFA levels were observed in rats fed HVF, which contributed to the development of fatty liver. The RUM diet led to higher concentrations of stearic acid and conjugated linoleic acid (CLA). The lower incorporation of TFA and distinct FA profile in the liver of rats fed RUM compared to HVF could be associated with protection hepatic. The microbial gut communities were significantly dissimilar among the groups. It is plausible that ruminant fat might reduce risk parameters involved in the development of cardiovascular disease.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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