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Research Article
Nathália C.C. Silva
State University of Campinas
Corresponding Author
License:
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Background
Staphylococcus spp. are among the most isolated bacteria from cases of clinical and subclinical mastitis in dairy cattle. Also, Staphylococcus is commonly isolated from bovine mammary secretions, not exclusively from intramammary infections. The genus comprises bacteria capable to form biofilm, produce toxins and acquire multi-drug resistance. The aims of this work were to evaluate the genetic virulence and antimicrobial resistance features of Staphylococcus spp. isolated from bovine milk using whole genome sequencing, and build a phylogenetic tree with gene sequences of Staphylococcus spp. isolated from clinical mastitis and non-clinical fresh cows.
Results
The bacterial collection comprised 29 Staphylococcus strains isolated from clinical mastitis cases (n = 7) and milk samples collected from fresh cows (n = 22). Strains were identified as Staphylococcus aureus (n = 2), Staphylococcus chromogenes (n = 19), and Staphylococcus haemolyticus (n = 8). After sequences quality control, twenty-three strains had good quality of contigs to be included in the further analysis.
Conclusions
Ninety-four virulence genes were observed, including pvl, icaA, icaD and MMSCRAMS genes. We also detected important resistance genes as blaZ, ant(4), erm(B), fexA, lnu(D), tet(L) and tet(M). The phylogenetic tree related the species as expected and presented four clades.
Keywords
virulence, resistance, staphylococci, dairy cattle
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No competing interests reported.
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Nathália C.C. Silva
State University of Campinas
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 04 Mar, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Large Animal Medicine
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Staphylococcus spp. are among the most isolated bacteria from cases of clinical and subclinical mastitis in dairy cattle. Also, Staphylococcus is commonly isolated from bovine mammary secretions, not exclusively from intramammary infections. The genus comprises bacteria capable to form biofilm, produce toxins and acquire multi-drug resistance. The aims of this work were to evaluate the genetic virulence and antimicrobial resistance features of Staphylococcus spp. isolated from bovine milk using whole genome sequencing, and build a phylogenetic tree with gene sequences of Staphylococcus spp. isolated from clinical mastitis and non-clinical fresh cows.
Results
The bacterial collection comprised 29 Staphylococcus strains isolated from clinical mastitis cases (n = 7) and milk samples collected from fresh cows (n = 22). Strains were identified as Staphylococcus aureus (n = 2), Staphylococcus chromogenes (n = 19), and Staphylococcus haemolyticus (n = 8). After sequences quality control, twenty-three strains had good quality of contigs to be included in the further analysis.
Conclusions
Ninety-four virulence genes were observed, including pvl, icaA, icaD and MMSCRAMS genes. We also detected important resistance genes as blaZ, ant(4), erm(B), fexA, lnu(D), tet(L) and tet(M). The phylogenetic tree related the species as expected and presented four clades.
Figure 1
Figure 2
No competing interests reported.
This is a list of supplementary files associated with this preprint. Click to download.
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