Staphylococcus spp. are among the most isolated bacteria from cases of clinical and subclinical mastitis in dairy cattle. Also, Staphylococcus is commonly isolated from bovine mammary secretions, not exclusively from intramammary infections. The genus comprises bacteria capable to form biofilm, produce toxins and acquire multi-drug resistance. The aims of this work were to evaluate the genetic virulence and antimicrobial resistance features of Staphylococcus spp. isolated from bovine milk using whole genome sequencing, and build a phylogenetic tree with gene sequences of Staphylococcus spp. isolated from clinical mastitis and non-clinical fresh cows.
The bacterial collection comprised 29 Staphylococcus strains isolated from clinical mastitis cases (n = 7) and milk samples collected from fresh cows (n = 22). Strains were identified as Staphylococcus aureus (n = 2), Staphylococcus chromogenes (n = 19), and Staphylococcus haemolyticus (n = 8). After sequences quality control, twenty-three strains had good quality of contigs to be included in the further analysis.
Ninety-four virulence genes were observed, including pvl, icaA, icaD and MMSCRAMS genes. We also detected important resistance genes as blaZ, ant(4), erm(B), fexA, lnu(D), tet(L) and tet(M). The phylogenetic tree related the species as expected and presented four clades.
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No competing interests reported.
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Posted 04 Mar, 2021
Posted 04 Mar, 2021
Staphylococcus spp. are among the most isolated bacteria from cases of clinical and subclinical mastitis in dairy cattle. Also, Staphylococcus is commonly isolated from bovine mammary secretions, not exclusively from intramammary infections. The genus comprises bacteria capable to form biofilm, produce toxins and acquire multi-drug resistance. The aims of this work were to evaluate the genetic virulence and antimicrobial resistance features of Staphylococcus spp. isolated from bovine milk using whole genome sequencing, and build a phylogenetic tree with gene sequences of Staphylococcus spp. isolated from clinical mastitis and non-clinical fresh cows.
The bacterial collection comprised 29 Staphylococcus strains isolated from clinical mastitis cases (n = 7) and milk samples collected from fresh cows (n = 22). Strains were identified as Staphylococcus aureus (n = 2), Staphylococcus chromogenes (n = 19), and Staphylococcus haemolyticus (n = 8). After sequences quality control, twenty-three strains had good quality of contigs to be included in the further analysis.
Ninety-four virulence genes were observed, including pvl, icaA, icaD and MMSCRAMS genes. We also detected important resistance genes as blaZ, ant(4), erm(B), fexA, lnu(D), tet(L) and tet(M). The phylogenetic tree related the species as expected and presented four clades.
Figure 1
Figure 2
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