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Research Article
Satoshi Konishi
Ritsumeikan University
Corresponding Author
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On-chip technology continuously explores and provides novel methods for high-throughput drug screening, such as droplet-array sandwiching technology. Droplet-array sandwiching technology involves upper and lower substrates with a droplet-array designed for a one-step process for drug screening, including cell-based screening. This technology is, however, limited to batch manipulation of the droplet-array. This paper proposes selective control of individual droplets, which allows different conditions for individual droplet pairs. Electrowetting-on-dielectric (EWOD) technology is introduced to control the height of the droplets so that the contact between droplet-pairs can be individually controlled. Circular patterns 4 mm in diameter composed of electrodes for EWOD and hydrophilic-hydrophobic patterns for droplet formation 4 μl in volume were developed. This paper presents and examines the selective control of the droplet height by EWOD for an applied voltage up to 160 V, followed by successful results of selective control of the contact and transport of substances.
Keywords
lab-on-a-chip (LOC), electrowetting-on-dielectric (EWOD) technology
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No competing interests reported.
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On 12 Apr, 2021
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Received 07 Apr, 2021
Reviewer #9 agreed
On 31 Mar, 2021
Reviewer #1 agreed
On 31 Mar, 2021
Reviewer #2 agreed
On 31 Mar, 2021
Reviewer #10 agreed
On 31 Mar, 2021
Reviewer #7 agreed
On 31 Mar, 2021
Reviewer #8 agreed
On 31 Mar, 2021
Reviewer #6 agreed
On 31 Mar, 2021
Reviewer #5 agreed
On 31 Mar, 2021
Reviewer #3 agreed
On 31 Mar, 2021
Reviewer #4 agreed
On 31 Mar, 2021
Reviewers invited
Invitations sent on 31 Mar, 2021
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Editor invited
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This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Satoshi Konishi
Ritsumeikan University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Revision
Version 1
Posted 04 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 12 Apr, 2021
Review #3 received
Received 07 Apr, 2021
Review #4 received
Received 07 Apr, 2021
Review #1 received
Received 07 Apr, 2021
Review #2 received
Received 07 Apr, 2021
Reviewer #9 agreed
On 31 Mar, 2021
Reviewer #1 agreed
On 31 Mar, 2021
Reviewer #2 agreed
On 31 Mar, 2021
Reviewer #10 agreed
On 31 Mar, 2021
Reviewer #7 agreed
On 31 Mar, 2021
Reviewer #8 agreed
On 31 Mar, 2021
Reviewer #6 agreed
On 31 Mar, 2021
Reviewer #5 agreed
On 31 Mar, 2021
Reviewer #3 agreed
On 31 Mar, 2021
Reviewer #4 agreed
On 31 Mar, 2021
Reviewers invited
Invitations sent on 31 Mar, 2021
Editor assigned
On 31 Mar, 2021
Editor invited
On 02 Mar, 2021
Submission checks complete
On 02 Mar, 2021
First submitted
On 22 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
On-chip technology continuously explores and provides novel methods for high-throughput drug screening, such as droplet-array sandwiching technology. Droplet-array sandwiching technology involves upper and lower substrates with a droplet-array designed for a one-step process for drug screening, including cell-based screening. This technology is, however, limited to batch manipulation of the droplet-array. This paper proposes selective control of individual droplets, which allows different conditions for individual droplet pairs. Electrowetting-on-dielectric (EWOD) technology is introduced to control the height of the droplets so that the contact between droplet-pairs can be individually controlled. Circular patterns 4 mm in diameter composed of electrodes for EWOD and hydrophilic-hydrophobic patterns for droplet formation 4 μl in volume were developed. This paper presents and examines the selective control of the droplet height by EWOD for an applied voltage up to 160 V, followed by successful results of selective control of the contact and transport of substances.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
This preprint is available for download as a PDF.
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