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Research Article
Kuo Liu
Capital Medical University
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Background: CVD is the leading cause of death in T2DM patients. However, few biomarkers have been identified to detect and diagnose CVD in the early stage of T2DM. The aim of our study was to identify the important mRNAs, micro (mi)RNAs and SNPs (single nucleotide polymorphisms) that are associated with metabolic cardiovascular disease.
Materials and methods: Expression profiles and GWAS data were obtained from Gene Expression Omnibus (GEO) database. MiRNA-sequencing was conducted by Illumina HiSeq 2000 platform in T2DM patients and T2DM with CVD patients. EQTL analysis and gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted. MRNA-miRNA co-expression network and mRNA-SNP-miRNA interaction network were established and visualized by Cytoscape 3.7.2.
Results: In our study, we identified 56 genes and 16 miRNAs that were significantly differentially expressed. GO and KEGG analyses results indicated that B cell receptor signaling pathway and hematopoietic cell lineage were included in the biological functions of differentially expressed genes. MRNA-miRNA co-expression network and mRNA-SNP-miRNA interaction network illustrated that let-7i-5p, RASGRP3, KRT1 and CEP41 may be potential biomarkers for the early detection and diagnosis of CVD in T2DM patients.
Conclusion: Our results suggested that downregulated let-7i-5p, and upregulated RASGRP3, KRT1 and CEP41 may play crucial roles in molecular mechanisms underlying the initiation and development of CVD in T2DM patients.
Keywords
CVD, T2DM, mRNA, SNP, miRNA, interaction network
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No competing interests reported.
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Reviewer #12 agreed
On 01 Mar, 2021
Reviewer #9 agreed
On 01 Mar, 2021
Reviewer #10 agreed
On 01 Mar, 2021
Reviewer #13 agreed
On 01 Mar, 2021
Reviewer #11 agreed
On 01 Mar, 2021
Reviewer #6 agreed
On 01 Mar, 2021
Reviewer #1 agreed
On 01 Mar, 2021
Reviewer #2 agreed
On 01 Mar, 2021
Reviewer #8 agreed
On 01 Mar, 2021
Reviewers invited
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Editor assigned
On 25 Feb, 2021
Editor invited
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Subject Areas
Cardiac & Cardiovascular Systems
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This preprint is under consideration at BMC Cardiovascular Disorders. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Kuo Liu
Capital Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 04 Mar, 2021
No community comments so far
Review #11 received
Received 06 Mar, 2021
Review #8 received
Received 06 Mar, 2021
Review #9 received
Received 06 Mar, 2021
Review #12 received
Received 06 Mar, 2021
Review #10 received
Received 06 Mar, 2021
Review #5 received
Received 06 Mar, 2021
Review #1 received
Received 06 Mar, 2021
Review #7 received
Received 06 Mar, 2021
Reviewer #12 agreed
On 01 Mar, 2021
Reviewer #9 agreed
On 01 Mar, 2021
Reviewer #10 agreed
On 01 Mar, 2021
Reviewer #13 agreed
On 01 Mar, 2021
Reviewer #11 agreed
On 01 Mar, 2021
Reviewer #6 agreed
On 01 Mar, 2021
Reviewer #1 agreed
On 01 Mar, 2021
Reviewer #2 agreed
On 01 Mar, 2021
Reviewer #8 agreed
On 01 Mar, 2021
Reviewers invited
Invitations sent on 25 Feb, 2021
Editor assigned
On 25 Feb, 2021
Editor invited
On 25 Feb, 2021
Submission checks complete
On 25 Feb, 2021
First submitted
On 22 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cardiac & Cardiovascular Systems
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: CVD is the leading cause of death in T2DM patients. However, few biomarkers have been identified to detect and diagnose CVD in the early stage of T2DM. The aim of our study was to identify the important mRNAs, micro (mi)RNAs and SNPs (single nucleotide polymorphisms) that are associated with metabolic cardiovascular disease.
Materials and methods: Expression profiles and GWAS data were obtained from Gene Expression Omnibus (GEO) database. MiRNA-sequencing was conducted by Illumina HiSeq 2000 platform in T2DM patients and T2DM with CVD patients. EQTL analysis and gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted. MRNA-miRNA co-expression network and mRNA-SNP-miRNA interaction network were established and visualized by Cytoscape 3.7.2.
Results: In our study, we identified 56 genes and 16 miRNAs that were significantly differentially expressed. GO and KEGG analyses results indicated that B cell receptor signaling pathway and hematopoietic cell lineage were included in the biological functions of differentially expressed genes. MRNA-miRNA co-expression network and mRNA-SNP-miRNA interaction network illustrated that let-7i-5p, RASGRP3, KRT1 and CEP41 may be potential biomarkers for the early detection and diagnosis of CVD in T2DM patients.
Conclusion: Our results suggested that downregulated let-7i-5p, and upregulated RASGRP3, KRT1 and CEP41 may play crucial roles in molecular mechanisms underlying the initiation and development of CVD in T2DM patients.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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