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Research article
Hong Zhang
Shengjing Hospital of China Medical University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
To investigate the effects of miR-200c targeting fucosyltransferase 4 (FUT4) on the proliferation, migration and invasion of colon cancer cells and further to explore its mechanism.
Methods
The expression of miR-200c and FUT4 mRNA in Lovo and SW480 cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR), and their correlation was analyzed by Pearson. LipofectamineTM 2000 transfection reagent was used to transfect miR-200c mimic, FUT4 siRNA, FUT4 mimic and FUT4 mimic negative control into Lovo and SW480 cells, and RT-PCR was used to analyze the effect of transfection. Cell counting kitcck-8 (CCK-8), cloning and transwell assays were used to detect the migration, invasion and proliferation of Lovo and SW480 cells, respectively. Immunofluorescence was used to analyze the expression of Ki-67 protein. Moreover, the expression of Wnt/β-catenin signaling pathway-related proteins were detected by western blot. Double luciferase experiment was performed to verify the targeting relationship between miR-200c and FUT4.
Results
Pearson results showed that miR-200c and FUT4 were negatively correlated in Lovo and SW480 cells (correlation coefficients were − 0.9046 and − 0.9236, respectively). MiR-200c overexpression inhibits the proliferation, migration and invasion of Lovo cells by down-regulating FUT4. The expression of Ki67 positive cells and Wnt/β-catenin signaling pathway-related proteins were reduced in miR-200c overexpression and FUT4 silencing groups. The scientific search and dual luciferase reporting system identified FUT4 was the target of miR-200c.
Conclusion
In conclusion, miR-200c overexpression inhibits FUT4 expression and down-regulates the Wnt/β-catenin signaling pathway, thereby inhibiting the migration, invasion and proliferation of colon cancer cells.
Keywords
miR-200c, FUT4, colon cancer, Wnt/β-catenin pathway
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View the published article at BMC Cancer.
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Editorial decision: Accept
On 18 Nov, 2020
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Editor assigned
On 07 Nov, 2020
Submission checks complete
On 07 Nov, 2020
Editor invited
On 07 Nov, 2020
No comments provided
Editorial decision: Minor revision
On 28 Oct, 2020
Editor assigned
On 12 Oct, 2020
Submission checks complete
On 11 Oct, 2020
Editor invited
On 11 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 25 Sep, 2020
Review #2 received
Received 23 Sep, 2020
Reviewer #2 agreed
On 17 Sep, 2020
Reviewer #1 agreed
On 15 Sep, 2020
Review #1 received
Received 15 Sep, 2020
Reviewers invited
Invitations sent on 14 Sep, 2020
Editor assigned
On 08 Sep, 2020
Submission checks complete
On 07 Sep, 2020
Editor invited
On 07 Sep, 2020
Version 1
Posted 27 May, 2020
No comments provided
Editorial decision: Major revision
On 20 Aug, 2020
Review #2 received
Received 17 Aug, 2020
Reviewer #2 agreed
On 29 Jul, 2020
Reviewer #3 agreed
On 29 Jul, 2020
Review #1 received
Received 24 Jun, 2020
Reviewer #1 agreed
On 04 Jun, 2020
Editor assigned
On 21 May, 2020
Reviewers invited
Invitations sent on 21 May, 2020
Submission checks complete
On 20 May, 2020
Editor invited
On 14 May, 2020
First submitted
On 06 May, 2020
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A new preprint design is coming!
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See the published version of this preprint at BMC Cancer.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Hong Zhang
Shengjing Hospital of China Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Cancer.
No community comments so far
Submission checks complete
On 21 Nov, 2020
Editorial decision: Accept
On 18 Nov, 2020
No comments provided
Editor assigned
On 07 Nov, 2020
Submission checks complete
On 07 Nov, 2020
Editor invited
On 07 Nov, 2020
No comments provided
Editorial decision: Minor revision
On 28 Oct, 2020
Editor assigned
On 12 Oct, 2020
Submission checks complete
On 11 Oct, 2020
Editor invited
On 11 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 25 Sep, 2020
Review #2 received
Received 23 Sep, 2020
Reviewer #2 agreed
On 17 Sep, 2020
Reviewer #1 agreed
On 15 Sep, 2020
Review #1 received
Received 15 Sep, 2020
Reviewers invited
Invitations sent on 14 Sep, 2020
Editor assigned
On 08 Sep, 2020
Submission checks complete
On 07 Sep, 2020
Editor invited
On 07 Sep, 2020
Version 1
Posted 27 May, 2020
No comments provided
Editorial decision: Major revision
On 20 Aug, 2020
Review #2 received
Received 17 Aug, 2020
Reviewer #2 agreed
On 29 Jul, 2020
Reviewer #3 agreed
On 29 Jul, 2020
Review #1 received
Received 24 Jun, 2020
Reviewer #1 agreed
On 04 Jun, 2020
Editor assigned
On 21 May, 2020
Reviewers invited
Invitations sent on 21 May, 2020
Submission checks complete
On 20 May, 2020
Editor invited
On 14 May, 2020
First submitted
On 06 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Cancer.
Background
To investigate the effects of miR-200c targeting fucosyltransferase 4 (FUT4) on the proliferation, migration and invasion of colon cancer cells and further to explore its mechanism.
Methods
The expression of miR-200c and FUT4 mRNA in Lovo and SW480 cells were detected by quantitative real-time polymerase chain reaction (qRT-PCR), and their correlation was analyzed by Pearson. LipofectamineTM 2000 transfection reagent was used to transfect miR-200c mimic, FUT4 siRNA, FUT4 mimic and FUT4 mimic negative control into Lovo and SW480 cells, and RT-PCR was used to analyze the effect of transfection. Cell counting kitcck-8 (CCK-8), cloning and transwell assays were used to detect the migration, invasion and proliferation of Lovo and SW480 cells, respectively. Immunofluorescence was used to analyze the expression of Ki-67 protein. Moreover, the expression of Wnt/β-catenin signaling pathway-related proteins were detected by western blot. Double luciferase experiment was performed to verify the targeting relationship between miR-200c and FUT4.
Results
Pearson results showed that miR-200c and FUT4 were negatively correlated in Lovo and SW480 cells (correlation coefficients were − 0.9046 and − 0.9236, respectively). MiR-200c overexpression inhibits the proliferation, migration and invasion of Lovo cells by down-regulating FUT4. The expression of Ki67 positive cells and Wnt/β-catenin signaling pathway-related proteins were reduced in miR-200c overexpression and FUT4 silencing groups. The scientific search and dual luciferase reporting system identified FUT4 was the target of miR-200c.
Conclusion
In conclusion, miR-200c overexpression inhibits FUT4 expression and down-regulates the Wnt/β-catenin signaling pathway, thereby inhibiting the migration, invasion and proliferation of colon cancer cells.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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