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Original article
Qirong Shen
Nanjing Agricultural University
Corresponding Author
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Fusarium wilt is a devastating disease which impacts watermelon production. Soil fumigation using dazomet followed by biological organic fertilizer was applied to suppress the Fusarium wilt disease. We propose that fumigation suppresses the soil indigenous community, especially the soil-borne disease pathogens, while the utilization of bio-organic fertilizer facilitates the recovery of the soil microbiome to a beneficial, suppressive state through the introduction of plant growth-promoting microorganisms. Greenhouse experiments showed that applied biological organic fertilizer after dazomet fumigation effective restrain the disease incidence with a 93.6% disease control. Fumigation strongly decreased soil microbial diversity and altered relative taxa abundances, suggesting the possibility of niche release by the resident soil microbial community. Fumigation followed by bio-fertilizer transformed the soil microbial community composition and resulted in higher relative abundances of beneficial microbial groups such as Bacillus (8.5 %) and Trichoderma (13.5 %), coupled with lower Fusarium abundance compared to other treatments. Network analysis illustrated that soil fumigation decreased interactions within the soil microbial community with less nodes and links while bio-fertilizer addition promoted node interactions. In addition, bio-fertilizer addition after fumigation resulted in the beneficial species becoming the key network connectors. Collectively, fumigation appears to release the resident soil niche resulting in lower diversity while the beneficial microbes introduced by bio-fertilizer addition colonize these niches, leading to a more complex community with fewer pathogens that suppresses Fusarium wilt disease incidence.
Keywords
Watermelon Fusarium wilt disease, Soil microbial resistance and resilience, Dazomet fumigation, Biological organic fertilizer, Soil microbial assembly
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Reviewer #3 agreed
On 23 Apr, 2021
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Received 23 Apr, 2021
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On 20 Apr, 2021
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Received 20 Apr, 2021
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On 19 Apr, 2021
Reviewers invited
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Review #1 received
Received 25 Apr, 2021
Reviewer #3 agreed
On 23 Apr, 2021
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Received 23 Apr, 2021
Reviewer #2 agreed
On 20 Apr, 2021
Review #2 received
Received 20 Apr, 2021
Editor assigned
On 19 Apr, 2021
Reviewers invited
Invitations sent on 19 Apr, 2021
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On 19 Apr, 2021
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Editor invited
On 19 Apr, 2021
This version was not preprinted
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Posted 25 Apr, 2021
Review #1 received
Received 25 Apr, 2021
Review #3 received
Received 23 Apr, 2021
Reviews received
Received 23 Apr, 2021
Reviewer #3 agreed
On 23 Apr, 2021
Reviewer #2 agreed
On 20 Apr, 2021
Review #2 received
Received 20 Apr, 2021
Editor assigned
On 19 Apr, 2021
Reviewers invited
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On 19 Apr, 2021
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On 19 Apr, 2021
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Reviewer #3 agreed
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This preprint is under consideration at AMB Express. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Original article
Qirong Shen
Nanjing Agricultural University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version (no preprint)
Posted 26 Apr, 2021
Review #1 received
Received 25 Apr, 2021
Reviewer #3 agreed
On 23 Apr, 2021
Review #3 received
Received 23 Apr, 2021
Reviewer #2 agreed
On 20 Apr, 2021
Review #2 received
Received 20 Apr, 2021
Editor assigned
On 19 Apr, 2021
Reviewers invited
Invitations sent on 19 Apr, 2021
Reviewer #1 agreed
On 19 Apr, 2021
Submission checks complete
On 19 Apr, 2021
Editor invited
On 19 Apr, 2021
This version was not preprinted
Version (no preprint)
Posted 26 Apr, 2021
Review #1 received
Received 25 Apr, 2021
Submission checks complete
On 25 Apr, 2021
Editor invited
On 25 Apr, 2021
Reviewer #3 agreed
On 23 Apr, 2021
Review #3 received
Received 23 Apr, 2021
Reviewer #2 agreed
On 20 Apr, 2021
Review #2 received
Received 20 Apr, 2021
Reviews received
Received 20 Apr, 2021
Reviewer #1 agreed
On 19 Apr, 2021
Editor assigned
On 19 Apr, 2021
Reviewers invited
Invitations sent on 19 Apr, 2021
First submitted
On 08 Apr, 2021
This version was not preprinted
Version (no preprint)
Posted 26 Apr, 2021
Review #1 received
Received 25 Apr, 2021
Reviewer #3 agreed
On 23 Apr, 2021
Review #3 received
Received 23 Apr, 2021
Reviewer #2 agreed
On 20 Apr, 2021
Review #2 received
Received 20 Apr, 2021
Editor assigned
On 19 Apr, 2021
Reviewers invited
Invitations sent on 19 Apr, 2021
Reviewer #1 agreed
On 19 Apr, 2021
Submission checks complete
On 19 Apr, 2021
Editor invited
On 19 Apr, 2021
This version was not preprinted
Version (no preprint)
Posted 26 Apr, 2021
Review #1 received
Received 25 Apr, 2021
Reviewer #3 agreed
On 23 Apr, 2021
Review #3 received
Received 23 Apr, 2021
Reviewer #2 agreed
On 20 Apr, 2021
Review #2 received
Received 20 Apr, 2021
Editor assigned
On 19 Apr, 2021
Reviewers invited
Invitations sent on 19 Apr, 2021
Reviewer #1 agreed
On 19 Apr, 2021
Submission checks complete
On 19 Apr, 2021
Editor invited
On 19 Apr, 2021
This version was not preprinted
Version (no preprint)
Posted 25 Apr, 2021
Review #1 received
Received 25 Apr, 2021
Review #3 received
Received 23 Apr, 2021
Reviews received
Received 23 Apr, 2021
Reviewer #3 agreed
On 23 Apr, 2021
Reviewer #2 agreed
On 20 Apr, 2021
Review #2 received
Received 20 Apr, 2021
Editor assigned
On 19 Apr, 2021
Reviewers invited
Invitations sent on 19 Apr, 2021
Reviewer #1 agreed
On 19 Apr, 2021
Submission checks complete
On 19 Apr, 2021
Editor invited
On 19 Apr, 2021
First submitted
On 19 Apr, 2021
This version was not preprinted
Version 2
Posted 15 Mar, 2021
No community comments so far
Editorial decision: Minor Revision
On 24 Mar, 2021
Review #3 received
Received 23 Mar, 2021
Review #2 received
Received 18 Mar, 2021
Review #1 received
Received 16 Mar, 2021
Reviewer #3 agreed
On 08 Mar, 2021
Reviewer #2 agreed
On 07 Mar, 2021
Reviewers invited
Invitations sent on 07 Mar, 2021
Reviewer #1 agreed
On 07 Mar, 2021
Reviews received
Received 07 Mar, 2021
Editor assigned
On 04 Mar, 2021
Submission checks complete
On 04 Mar, 2021
Editor invited
On 04 Mar, 2021
First submitted
On 02 Mar, 2021
No comments provided
Editorial decision: Reject-invited to resubmit
On 25 Feb, 2021
Editor assigned
On 24 Feb, 2021
Submission checks complete
On 24 Feb, 2021
Editor invited
On 24 Feb, 2021
First submitted
On 22 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Fusarium wilt is a devastating disease which impacts watermelon production. Soil fumigation using dazomet followed by biological organic fertilizer was applied to suppress the Fusarium wilt disease. We propose that fumigation suppresses the soil indigenous community, especially the soil-borne disease pathogens, while the utilization of bio-organic fertilizer facilitates the recovery of the soil microbiome to a beneficial, suppressive state through the introduction of plant growth-promoting microorganisms. Greenhouse experiments showed that applied biological organic fertilizer after dazomet fumigation effective restrain the disease incidence with a 93.6% disease control. Fumigation strongly decreased soil microbial diversity and altered relative taxa abundances, suggesting the possibility of niche release by the resident soil microbial community. Fumigation followed by bio-fertilizer transformed the soil microbial community composition and resulted in higher relative abundances of beneficial microbial groups such as Bacillus (8.5 %) and Trichoderma (13.5 %), coupled with lower Fusarium abundance compared to other treatments. Network analysis illustrated that soil fumigation decreased interactions within the soil microbial community with less nodes and links while bio-fertilizer addition promoted node interactions. In addition, bio-fertilizer addition after fumigation resulted in the beneficial species becoming the key network connectors. Collectively, fumigation appears to release the resident soil niche resulting in lower diversity while the beneficial microbes introduced by bio-fertilizer addition colonize these niches, leading to a more complex community with fewer pathogens that suppresses Fusarium wilt disease incidence.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
This is a list of supplementary files associated with this preprint. Click to download.
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