Background
WD40 repeat (WDR)43 is an RNA-binding protein that belongs to the WDR domain protein family. Its biological function is largely unclear, particularly in colorectal cancer (CRC).
Methods
In the present study, we searched the TCGA database and found the correlation between WDR43 and CRC. Subsequently,the high expression of WDR43 in human clinical samples of CRC was validated and we further examined the biological functions of it in CRC cells.Finally,we explored potential downstream proteins or pathways and established subcutaneous xenograft model to verify our findings.
Results
Immunohistochemistry of 16 patient specimens confirmed that the expression of WDR43 was elevated in CRC. WDR43 knockdown was shown to increase apoptosis and inhibit the proliferation, migration and invasion of CRC cells in vitro and reduce tumorigenesis in animal models.In addition, it was found that WDR43 knockdown inhibited vimentin (VIM) expression in CRC cells and overexpression of VIM can partially reverse the effects of WDR43 both in vitro and in vivo.
Conclusion
In conclusion, the role of WDR43 in the occurrence and development of CRC was investigated in the present study. WDR43 may serve as a valuable biomarker and provide new options for the diagnosis and treatment of colorectal cancer.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
This is a list of supplementary files associated with this preprint. Click to download.
Loading...
Posted 04 Mar, 2021
On 24 Mar, 2021
Received 23 Mar, 2021
Received 14 Mar, 2021
On 09 Mar, 2021
Received 04 Mar, 2021
On 04 Mar, 2021
Invitations sent on 25 Feb, 2021
On 24 Feb, 2021
On 22 Feb, 2021
On 22 Feb, 2021
On 22 Feb, 2021
Posted 04 Mar, 2021
On 24 Mar, 2021
Received 23 Mar, 2021
Received 14 Mar, 2021
On 09 Mar, 2021
Received 04 Mar, 2021
On 04 Mar, 2021
Invitations sent on 25 Feb, 2021
On 24 Feb, 2021
On 22 Feb, 2021
On 22 Feb, 2021
On 22 Feb, 2021
Background
WD40 repeat (WDR)43 is an RNA-binding protein that belongs to the WDR domain protein family. Its biological function is largely unclear, particularly in colorectal cancer (CRC).
Methods
In the present study, we searched the TCGA database and found the correlation between WDR43 and CRC. Subsequently,the high expression of WDR43 in human clinical samples of CRC was validated and we further examined the biological functions of it in CRC cells.Finally,we explored potential downstream proteins or pathways and established subcutaneous xenograft model to verify our findings.
Results
Immunohistochemistry of 16 patient specimens confirmed that the expression of WDR43 was elevated in CRC. WDR43 knockdown was shown to increase apoptosis and inhibit the proliferation, migration and invasion of CRC cells in vitro and reduce tumorigenesis in animal models.In addition, it was found that WDR43 knockdown inhibited vimentin (VIM) expression in CRC cells and overexpression of VIM can partially reverse the effects of WDR43 both in vitro and in vivo.
Conclusion
In conclusion, the role of WDR43 in the occurrence and development of CRC was investigated in the present study. WDR43 may serve as a valuable biomarker and provide new options for the diagnosis and treatment of colorectal cancer.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Loading...