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Research Article
Wenxian Guan
Nanjing Drum Tower Hospital: Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
WD40 repeat (WDR)43 is an RNA-binding protein that belongs to the WDR domain protein family. Its biological function is largely unclear, particularly in colorectal cancer (CRC).
Methods
In the present study, we searched the TCGA database and found the correlation between WDR43 and CRC. Subsequently,the high expression of WDR43 in human clinical samples of CRC was validated and we further examined the biological functions of it in CRC cells.Finally,we explored potential downstream proteins or pathways and established subcutaneous xenograft model to verify our findings.
Results
Immunohistochemistry of 16 patient specimens confirmed that the expression of WDR43 was elevated in CRC. WDR43 knockdown was shown to increase apoptosis and inhibit the proliferation, migration and invasion of CRC cells in vitro and reduce tumorigenesis in animal models.In addition, it was found that WDR43 knockdown inhibited vimentin (VIM) expression in CRC cells and overexpression of VIM can partially reverse the effects of WDR43 both in vitro and in vivo.
Conclusion
In conclusion, the role of WDR43 in the occurrence and development of CRC was investigated in the present study. WDR43 may serve as a valuable biomarker and provide new options for the diagnosis and treatment of colorectal cancer.
Keywords
WDR43, VIM, CRC, Biomarker
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Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 04 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 24 Mar, 2021
Review #2 received
Received 23 Mar, 2021
Review #1 received
Received 14 Mar, 2021
Reviewer #2 agreed
On 09 Mar, 2021
Reviews received
Received 04 Mar, 2021
Reviewer #1 agreed
On 04 Mar, 2021
Reviewers invited
Invitations sent on 25 Feb, 2021
Editor invited
On 24 Feb, 2021
First submitted
On 22 Feb, 2021
Editor assigned
On 22 Feb, 2021
Submission checks complete
On 22 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cancer Biology
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This preprint is under consideration at Cancer Cell International. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Wenxian Guan
Nanjing Drum Tower Hospital: Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 04 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 24 Mar, 2021
Review #2 received
Received 23 Mar, 2021
Review #1 received
Received 14 Mar, 2021
Reviewer #2 agreed
On 09 Mar, 2021
Reviews received
Received 04 Mar, 2021
Reviewer #1 agreed
On 04 Mar, 2021
Reviewers invited
Invitations sent on 25 Feb, 2021
Editor invited
On 24 Feb, 2021
First submitted
On 22 Feb, 2021
Editor assigned
On 22 Feb, 2021
Submission checks complete
On 22 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cancer Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
WD40 repeat (WDR)43 is an RNA-binding protein that belongs to the WDR domain protein family. Its biological function is largely unclear, particularly in colorectal cancer (CRC).
Methods
In the present study, we searched the TCGA database and found the correlation between WDR43 and CRC. Subsequently,the high expression of WDR43 in human clinical samples of CRC was validated and we further examined the biological functions of it in CRC cells.Finally,we explored potential downstream proteins or pathways and established subcutaneous xenograft model to verify our findings.
Results
Immunohistochemistry of 16 patient specimens confirmed that the expression of WDR43 was elevated in CRC. WDR43 knockdown was shown to increase apoptosis and inhibit the proliferation, migration and invasion of CRC cells in vitro and reduce tumorigenesis in animal models.In addition, it was found that WDR43 knockdown inhibited vimentin (VIM) expression in CRC cells and overexpression of VIM can partially reverse the effects of WDR43 both in vitro and in vivo.
Conclusion
In conclusion, the role of WDR43 in the occurrence and development of CRC was investigated in the present study. WDR43 may serve as a valuable biomarker and provide new options for the diagnosis and treatment of colorectal cancer.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
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