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Research
rui ling
Xijing Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7994-0964
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Breast cancer is the most common cancer diagnosed among women and is the second leading cause of cancer death. It is of great significance to explore potential candidate targets.
Methods: Cell function assays, siRNA, western blot, mass spectrum, flow cytometry, and other molecular biology techniques were conducted to verify the function of USP41 to breast cancer cell line.
Results: The results indicate that USP41 (ubiquitin-specific proteases 41) expression is positively related to breast cancer progression. USP41 overexpression greatly enhanced breast cancer colony-forming ability, proliferation and migration. In contrast, USP41 knockdown significantly inhibited breast cancer colony-forming ability, proliferation and migration. Moreover, association of USP41 with RACK1 (Receptor for activated C kinase 1) was proved by mass spectrum, indicating its potential role in TGF-β signaling.
Conclusions: USP41 can be a potential therapeutic target against breast cancer via RACK1.
Keywords
Ubiquitin-specific proteases 41, breast cancer, RACK1
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This is a preprint. It has not completed peer review.
Research
rui ling
Xijing Hospital
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7994-0964
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 06 Mar, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Breast cancer is the most common cancer diagnosed among women and is the second leading cause of cancer death. It is of great significance to explore potential candidate targets.
Methods: Cell function assays, siRNA, western blot, mass spectrum, flow cytometry, and other molecular biology techniques were conducted to verify the function of USP41 to breast cancer cell line.
Results: The results indicate that USP41 (ubiquitin-specific proteases 41) expression is positively related to breast cancer progression. USP41 overexpression greatly enhanced breast cancer colony-forming ability, proliferation and migration. In contrast, USP41 knockdown significantly inhibited breast cancer colony-forming ability, proliferation and migration. Moreover, association of USP41 with RACK1 (Receptor for activated C kinase 1) was proved by mass spectrum, indicating its potential role in TGF-β signaling.
Conclusions: USP41 can be a potential therapeutic target against breast cancer via RACK1.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
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