Wheat production is seriously predisposed by extreme hot weather, which has fascinated increasing attention. It is important to compare wheat responses to heat at seedling stages, to explore the potential relationship between the performances at different growing stages and the possibility of early selection to accelerate heat tolerance breeding. In this experiment, twenty wheat genotypes were used including two local landraces. The seed of wheat genotypes were sown on filter paper in petri dishes and allowed to germinate at different temperatures of 25°C, 30°C, 35°C and 40°C. The experiment was replicated thrice under complete randomized design (CRD) with 2 factors. Analysis of variance (ANOVA) for bio-chemical and seedling traits showed that the genotypes differed highly significant for coleoptile length, shoot length, root length, fresh shoot weight, vigor index, glycine betaine and proline content. Effect of treatments on genotypes was highly significantly different for vigor index. Principal component analysis (PCA) revealed that four factors contributed 82.8% to total variability with the Eigen value greater than 0.7 at 35°C. From correlation analysis it was concluded that coleoptile length and germination percentage had positively highly correlated with shoot length, vigor index and shoot fresh weight. On the basis overall performance among the twenty wheat genotypes, it was concluded that Maraj, Fareed, Darabi, Zincol-16, Barsat, NARC-2011 and genotype Mundar performed well under all temperature regimes. Varieties NARC-2011, Inqalab-91 and Galexy also performed well in the case of H2O2 and antioxidant activity. These genotypes can be used under extreme hot temperature and in future breeding programs as they had a significant level of variability.