Evaluation of Matrix Metalloproteinase-7 and Matrix Metalloproteinase-9 as Biomarkers in Ureteropelvic Junction Obstruction in Children- a preliminary report
In children with congenital ureteropelvic junction obstruction (UPJO), Urinary biomarker may provide understanding of the health of the kidney, the need to intervene surgically, and the expected response and recoverability of the kidney following successful surgery. We evaluate whether the urinary matrix metalloproteinase-7(MMP-7) and matrix metalloproteinase-9(MMP-9) might be served as biomarkers in UPJO patients.
In this study, 24 unilateral UPJO patients and 16 healthy childrens were enrolled. Of which, 20 patients underwent 99mTc-DTPA (Diethylenetriaminepentaacetic Acid) renal dynamic imaging. And 16 patients underwent pyeloplasty and 8 others were followed. Postoperative follow-ups included ultrasonography, as well as MMP-7 and MMP-9 levels in urine, six months and one year after pyeloplasty. Study population was divided in 4 categories according to Society of Fetal Urology classification. For DTPA we subdivided the UPJO patients in 2 groups, obstructive (T 1/2 more than 20 min) and partial obstructive (T 1/2 less than 20 min). Quantitative urine protein analysis was performed and urinary creatinine levels were used to normalize protein levels for the markers analyzed.
The results showed Creatinine-normalized urinary levels of MMP-7 and MMP-9 proteins were higher in patients with UPJO compared to healthy control subjects(P༜0.05). Moreover, urinary levels of MMP-7 and MMP-9 proteins in operation group were higher than non-operation group. Studies revealed that normalized urinary levels of MMP-7 and MMP-9 proteins in obstruction group were higher than partial obstruction group, although this differences were not statistically significant(P༞0.05). No significant differences in the Creatinine-normalized urinary levels of MMP-7 and MMP-9 in different degrees of hydronephrosis (P༞0.05). And normalized MMP-7 and MMP-9 levels were not correlated with the glomerular filtration rate of affected side(r = -0.128, P = 0.592; r = -0.157, P = 0.507).
Compared to control group, the levels of MMP-7 and MMP-9 in urine of unilateral UPJO patients are significantly elevated, and they can potentially be used as determining indices for surgical plan. Although preliminary results indicate no significant correlation between them and clinical parameters, they would be potential biomarkers for UPJO.
Figure 1
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Posted 19 May, 2020
Evaluation of Matrix Metalloproteinase-7 and Matrix Metalloproteinase-9 as Biomarkers in Ureteropelvic Junction Obstruction in Children- a preliminary report
Posted 19 May, 2020
In children with congenital ureteropelvic junction obstruction (UPJO), Urinary biomarker may provide understanding of the health of the kidney, the need to intervene surgically, and the expected response and recoverability of the kidney following successful surgery. We evaluate whether the urinary matrix metalloproteinase-7(MMP-7) and matrix metalloproteinase-9(MMP-9) might be served as biomarkers in UPJO patients.
In this study, 24 unilateral UPJO patients and 16 healthy childrens were enrolled. Of which, 20 patients underwent 99mTc-DTPA (Diethylenetriaminepentaacetic Acid) renal dynamic imaging. And 16 patients underwent pyeloplasty and 8 others were followed. Postoperative follow-ups included ultrasonography, as well as MMP-7 and MMP-9 levels in urine, six months and one year after pyeloplasty. Study population was divided in 4 categories according to Society of Fetal Urology classification. For DTPA we subdivided the UPJO patients in 2 groups, obstructive (T 1/2 more than 20 min) and partial obstructive (T 1/2 less than 20 min). Quantitative urine protein analysis was performed and urinary creatinine levels were used to normalize protein levels for the markers analyzed.
The results showed Creatinine-normalized urinary levels of MMP-7 and MMP-9 proteins were higher in patients with UPJO compared to healthy control subjects(P༜0.05). Moreover, urinary levels of MMP-7 and MMP-9 proteins in operation group were higher than non-operation group. Studies revealed that normalized urinary levels of MMP-7 and MMP-9 proteins in obstruction group were higher than partial obstruction group, although this differences were not statistically significant(P༞0.05). No significant differences in the Creatinine-normalized urinary levels of MMP-7 and MMP-9 in different degrees of hydronephrosis (P༞0.05). And normalized MMP-7 and MMP-9 levels were not correlated with the glomerular filtration rate of affected side(r = -0.128, P = 0.592; r = -0.157, P = 0.507).
Compared to control group, the levels of MMP-7 and MMP-9 in urine of unilateral UPJO patients are significantly elevated, and they can potentially be used as determining indices for surgical plan. Although preliminary results indicate no significant correlation between them and clinical parameters, they would be potential biomarkers for UPJO.
Figure 1