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Research article
Yashuang Zhao
Harbin Medical University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7425-5773
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Studies have shown that abnormal changes of specific-gene DNA methylation in leukocytes may be associated with an elevated risk of cancer. However, associations between the methylation of the zinc-related genes, WT1 and CA10, and breast cancer risk remain unknown.
Methods: The methylation of WT1 and CA10 was analyzed by methylation-sensitive high-resolution-melting (MS-HRM) in a case-control study with female subjects (N=959). Logistic regression was used to analyze the associations, and propensity score (PS) method was used to adjust confounders.
Results: The results showed that WT1 hypermethylation was associated with an increased risk of breast cancer, with an odds ratio (OR) of 3.07 [95% confidence interval (CI): 1.67-5.64, P<0.01]. Subgroup analyses showed that WT1 hypermethylation was specifically associated with an elevated risk of luminal A subtype (OR=2.62, 95% CI: 1.11-6.20, P=0.03) and luminal B subtype (OR=3.23, 95% CI: 1.34-7.80, P=0.01). CA10 hypermethylation was associated with an increased risk of luminal B subtype (OR=1.80, 95% CI: 1.09-2.98, P=0.02).
Conclusion: The results of the present study suggest that the hypermethylation of WT1 methylation in leukocytes is significantly associated with an increased risk of breast cancer. The hypermethylation of WT1 is associated with an increased risk of luminal subtypes of breast cancer, and the hypermethylation of CA10 is associated with an increased risk of luminal B subtype of breast cancer.
Keywords
breast cancer; CA10; WT1; DNA methylation; leukocytes.
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
- AdditionalFile1.docx
Table S1 Demographic variables and questionnaire-derived variables of participants before and after multiple imputation in this study
- AdditionalFile2.docx
Table S2. Primer sequences and reaction condition for methylation-sensitive high-resolution melting analysis
- AdditionalFile3.docx
Table S3. Reaction system for methylation-sensitive high-resolution melting analysis of WT1 and CA10
- AdditionalFile4.docx
Table S4 Result of methylation-sensitive high-resolution melting analysis for the same samples in different runs
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CURRENT STATUS: Published
View the published article at BMC Cancer.
Version 2
Posted 16 Jul, 2020
No community comments so far
Editorial decision: Accept
On 14 Jul, 2020
Reviewers invited
Invitations sent on 07 Jul, 2020
Reviewer #1 agreed
On 07 Jul, 2020
Reviewer #2 agreed
On 07 Jul, 2020
Review #1 received
Received 07 Jul, 2020
Review #2 received
Received 07 Jul, 2020
Editor assigned
On 07 Jul, 2020
Submission checks complete
On 06 Jul, 2020
Editor invited
On 06 Jul, 2020
No comments provided
Review #1 received
Received 06 Jun, 2020
Editorial decision: Major revision
On 06 Jun, 2020
Review #2 received
Received 02 Jun, 2020
Reviewer #2 agreed
On 27 May, 2020
Editor assigned
On 17 May, 2020
Reviewers invited
Invitations sent on 17 May, 2020
Reviewer #1 agreed
On 17 May, 2020
Submission checks complete
On 16 May, 2020
Editor invited
On 16 May, 2020
First submitted
On 05 May, 2020
Metrics
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PDF Downloads: ...
HTML Views: ...
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A new preprint design is coming!
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See the published version of this preprint at BMC Cancer.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Yashuang Zhao
Harbin Medical University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-7425-5773
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Cancer.
Version 2
Posted 16 Jul, 2020
No community comments so far
Editorial decision: Accept
On 14 Jul, 2020
Reviewers invited
Invitations sent on 07 Jul, 2020
Reviewer #1 agreed
On 07 Jul, 2020
Reviewer #2 agreed
On 07 Jul, 2020
Review #1 received
Received 07 Jul, 2020
Review #2 received
Received 07 Jul, 2020
Editor assigned
On 07 Jul, 2020
Submission checks complete
On 06 Jul, 2020
Editor invited
On 06 Jul, 2020
No comments provided
Review #1 received
Received 06 Jun, 2020
Editorial decision: Major revision
On 06 Jun, 2020
Review #2 received
Received 02 Jun, 2020
Reviewer #2 agreed
On 27 May, 2020
Editor assigned
On 17 May, 2020
Reviewers invited
Invitations sent on 17 May, 2020
Reviewer #1 agreed
On 17 May, 2020
Submission checks complete
On 16 May, 2020
Editor invited
On 16 May, 2020
First submitted
On 05 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Cancer.
Background: Studies have shown that abnormal changes of specific-gene DNA methylation in leukocytes may be associated with an elevated risk of cancer. However, associations between the methylation of the zinc-related genes, WT1 and CA10, and breast cancer risk remain unknown.
Methods: The methylation of WT1 and CA10 was analyzed by methylation-sensitive high-resolution-melting (MS-HRM) in a case-control study with female subjects (N=959). Logistic regression was used to analyze the associations, and propensity score (PS) method was used to adjust confounders.
Results: The results showed that WT1 hypermethylation was associated with an increased risk of breast cancer, with an odds ratio (OR) of 3.07 [95% confidence interval (CI): 1.67-5.64, P<0.01]. Subgroup analyses showed that WT1 hypermethylation was specifically associated with an elevated risk of luminal A subtype (OR=2.62, 95% CI: 1.11-6.20, P=0.03) and luminal B subtype (OR=3.23, 95% CI: 1.34-7.80, P=0.01). CA10 hypermethylation was associated with an increased risk of luminal B subtype (OR=1.80, 95% CI: 1.09-2.98, P=0.02).
Conclusion: The results of the present study suggest that the hypermethylation of WT1 methylation in leukocytes is significantly associated with an increased risk of breast cancer. The hypermethylation of WT1 is associated with an increased risk of luminal subtypes of breast cancer, and the hypermethylation of CA10 is associated with an increased risk of luminal B subtype of breast cancer.
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
- AdditionalFile1.docx
Table S1 Demographic variables and questionnaire-derived variables of participants before and after multiple imputation in this study
- AdditionalFile2.docx
Table S2. Primer sequences and reaction condition for methylation-sensitive high-resolution melting analysis
- AdditionalFile3.docx
Table S3. Reaction system for methylation-sensitive high-resolution melting analysis of WT1 and CA10
- AdditionalFile4.docx
Table S4 Result of methylation-sensitive high-resolution melting analysis for the same samples in different runs
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