This preprint is under consideration at Molecular Biology Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
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Research Article
Pan Li
Zhejiang University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9407-7740
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This work is licensed under a CC BY 4.0 License. Read Full License
Crossostephium chinense is a traditional Chinese medicinal herb and it is often cultivated as an ornamental plant. Previous studies on this species mainly focused on its chemical composition and it was represented rarely and marginally in genetic studies, which limited knowledge about its genetic background, and thus genomic resources remain scarce. To develop both chloroplast and nuclear polymorphic microsatellites for C. chinense, potential microsatellites were screened from genome skimming data of two individuals of C. chinense. Sixty-four and 63 cpSSR markers were identified from two chloroplast genomes of C. chinense. This study performed for the first-ever study on employment of genome skimming data and CandiSSR, consequently a total of 133 polymorphic nSSRs were developed. Ten nSSRs were randomly selected to test their transferability across 35 individuals from three populations of C. chinense, and 20 individuals each of Artemisia stolonifera and A. argyi. Cross-amplifications were successful done for C. chinense, and were partially successful amplified for both Artemisia species. The number of alleles varied from two to nine. The observed heterozygosity and expected heterozygosity per locus ranged from 0.000 to 0.286 and from 0.029 to 0.755, respectively. These genomic resources will be valuable for population genetics and conservation studies in C. chinense and Artemisia.
Keywords
Genome skimming, Simple sequence repeats (SSRs), Genetic resources, Molecular markers
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
- TableS1.docx
Locality and voucher information for populations of Crossostephium chinense, Artemisia stolonifera and A. argyi in this study.
- TableS2.xlsx
The detail information of polymorphic nSSRs identified for Crossostephium chinense and Primer pairs designed for each polymorphic nSSRs.
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This preprint is under consideration at Molecular Biology Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Pan Li
Zhejiang University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9407-7740
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 17 Mar, 2021
No community comments so far
Review #? received
Received 16 Mar, 2021
Reviewers invited
Invitations sent on 14 Mar, 2021
Editor assigned
On 28 Feb, 2021
First submitted
On 25 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Crossostephium chinense is a traditional Chinese medicinal herb and it is often cultivated as an ornamental plant. Previous studies on this species mainly focused on its chemical composition and it was represented rarely and marginally in genetic studies, which limited knowledge about its genetic background, and thus genomic resources remain scarce. To develop both chloroplast and nuclear polymorphic microsatellites for C. chinense, potential microsatellites were screened from genome skimming data of two individuals of C. chinense. Sixty-four and 63 cpSSR markers were identified from two chloroplast genomes of C. chinense. This study performed for the first-ever study on employment of genome skimming data and CandiSSR, consequently a total of 133 polymorphic nSSRs were developed. Ten nSSRs were randomly selected to test their transferability across 35 individuals from three populations of C. chinense, and 20 individuals each of Artemisia stolonifera and A. argyi. Cross-amplifications were successful done for C. chinense, and were partially successful amplified for both Artemisia species. The number of alleles varied from two to nine. The observed heterozygosity and expected heterozygosity per locus ranged from 0.000 to 0.286 and from 0.029 to 0.755, respectively. These genomic resources will be valuable for population genetics and conservation studies in C. chinense and Artemisia.
Figure 1
Figure 2
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