This preprint is under consideration at BMC Medical Genomics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
LncRNA sequencing analysis of cervical cancer identifies radiotherapy sensitivity markers
Yannan Shen
Jilin University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Cervical cancer is one of the most common human cancers worldwide. However, the treatment of cervical cancer does not meet patient expectations. In recent years, long noncoding RNAs (lncRNAs), which are nonprotein-coding transcripts, have been shown to play important roles in tumorigenesis and tumor progression.
Methods
The function and mechanism of lncRNAs in cervical carcinoma are still rarely reported. We selected 15 patients with cervical cancer and a good prognosis (RS) and 15 patients with cervical cancer and a poor prognosis (RR), and we then performed lncRNA Sequencing analysis to detect differentially expressed lncRNAs. Subsequently, we used online bioinformatics databases to explore the potential function of these dysregulated lncRNAs. Finally, quantitative real-time PCR was performed to confirm the expression levels of these dysregulated lncRNAs in sub-RR and RS samples.
Results
We used lncRNA Sequencing technology to reveal differentially expression between the lncRNA profiles of the RR and RS tissues, and we found 1097 upregulated and 1189 downregulated lncRNAs in cervical cancer (fold change > 2.0), 247 upregulated and 189 downregulated lncRNAs in cervical cancer (fold change > 5.0), and 94 upregulated and 95 downregulated lncRNAs in cervical cancer (fold change > 10.0).
Conclusions
This study provides a map of lncRNA expression in RR and RS tissues and provides a database for further study of the function and mechanisms of action of key lncRNAs in cervical cancer. LncRNA TCONS_00081487 regulate CD55 and increase the radiation sensitivity of cervical cancer.
Keywords
cervical cancer, lncRNA, mRNA, lncRNA sequencing, radiotherapy sensitivity
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 29 May, 2020
No community comments so far
Editorial decision: Reject after review
On 20 Jan, 2021
Review #1 received
Received 21 Nov, 2020
Reviewer #1 agreed
On 17 Jun, 2020
Reviewers invited
Invitations sent on 12 Jun, 2020
Editor assigned
On 20 May, 2020
Submission checks complete
On 19 May, 2020
Editor invited
On 19 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
Learn more about our company.
This preprint is under consideration at BMC Medical Genomics. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
LncRNA sequencing analysis of cervical cancer identifies radiotherapy sensitivity markers
Yannan Shen
Jilin University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 29 May, 2020
No community comments so far
Editorial decision: Reject after review
On 20 Jan, 2021
Review #1 received
Received 21 Nov, 2020
Reviewer #1 agreed
On 17 Jun, 2020
Reviewers invited
Invitations sent on 12 Jun, 2020
Editor assigned
On 20 May, 2020
Submission checks complete
On 19 May, 2020
Editor invited
On 19 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Cervical cancer is one of the most common human cancers worldwide. However, the treatment of cervical cancer does not meet patient expectations. In recent years, long noncoding RNAs (lncRNAs), which are nonprotein-coding transcripts, have been shown to play important roles in tumorigenesis and tumor progression.
Methods
The function and mechanism of lncRNAs in cervical carcinoma are still rarely reported. We selected 15 patients with cervical cancer and a good prognosis (RS) and 15 patients with cervical cancer and a poor prognosis (RR), and we then performed lncRNA Sequencing analysis to detect differentially expressed lncRNAs. Subsequently, we used online bioinformatics databases to explore the potential function of these dysregulated lncRNAs. Finally, quantitative real-time PCR was performed to confirm the expression levels of these dysregulated lncRNAs in sub-RR and RS samples.
Results
We used lncRNA Sequencing technology to reveal differentially expression between the lncRNA profiles of the RR and RS tissues, and we found 1097 upregulated and 1189 downregulated lncRNAs in cervical cancer (fold change > 2.0), 247 upregulated and 189 downregulated lncRNAs in cervical cancer (fold change > 5.0), and 94 upregulated and 95 downregulated lncRNAs in cervical cancer (fold change > 10.0).
Conclusions
This study provides a map of lncRNA expression in RR and RS tissues and provides a database for further study of the function and mechanisms of action of key lncRNAs in cervical cancer. LncRNA TCONS_00081487 regulate CD55 and increase the radiation sensitivity of cervical cancer.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8