This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research article
Association of Oral Microbiota with Sugar-Sweetened Beverages Consumption and Oral Health Problems in School-Aged Children
Jiao Fang
Anhui Medical University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6508-1433
Yuanyin Wang
Anhui Medical University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Consumption of beverages is prevalent among children and adolescents, of which the most popular beverages were sugar-sweetened beverages (SSBs). There is evidence that common beverage consumption, like coffee and tea, is associated with oral ecosystem. However, little is known about the effect of SSBs on the composition and functional potential of childhood oral microbiota.To examine the associations between SSBs consumption with oral microbiota diversity among school-aged children.Data came from the fourth wave follow-up of an ongoing child growth and development cohort established in 2016. Oral microbiota in buccal cheek swab samples were collected from 180 children (78 boys and 112 girls, aged 11.33±0.55 years old) using 16S rDNA gene sequencing. Students’ questionnaire was used to collect SSB consumption. Oral health problems, such as dental caries, gingivitis, was examined by a professional dentist. Higher SSBs consumption (≥ 1 serving/week) was associated with lower oral microbiota richness and diversity. Children with higher SSBs consumption showed altered abundance of several oral microbiota at genus level, including increases in Streptococcus, Gemella, Neisseria,Capnocytophaga and Lautropia (and decreases in Fusobacterium, Lachnoanaerobaculum, Soonwooa, Tannerella and Moraxella (p< 0.05). Functional analysis illustrated that oral microbiota was more conducive to the pathway activated of lysine biosynthesis (p=0.045), protein export (p=0.020), D-glutamine and D-glutamate metabolism (p=0.013), as well as pantothenate and CoA biosynthesis (p=0.004) in children with higher SSBs intake. Our finding suggests that higher SSBs consumption may disturb oral microecology and reduce diversity and abundance of oral microbiota during childhood.
Keywords
Sugar-sweetened beverages, Oral microbiota, Oral health problems
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 13 May, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Learn more about our company.
This is a preprint. It has not completed peer review.
Research article
Association of Oral Microbiota with Sugar-Sweetened Beverages Consumption and Oral Health Problems in School-Aged Children
Jiao Fang
Anhui Medical University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6508-1433
Yuanyin Wang
Anhui Medical University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 13 May, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Consumption of beverages is prevalent among children and adolescents, of which the most popular beverages were sugar-sweetened beverages (SSBs). There is evidence that common beverage consumption, like coffee and tea, is associated with oral ecosystem. However, little is known about the effect of SSBs on the composition and functional potential of childhood oral microbiota.To examine the associations between SSBs consumption with oral microbiota diversity among school-aged children.Data came from the fourth wave follow-up of an ongoing child growth and development cohort established in 2016. Oral microbiota in buccal cheek swab samples were collected from 180 children (78 boys and 112 girls, aged 11.33±0.55 years old) using 16S rDNA gene sequencing. Students’ questionnaire was used to collect SSB consumption. Oral health problems, such as dental caries, gingivitis, was examined by a professional dentist. Higher SSBs consumption (≥ 1 serving/week) was associated with lower oral microbiota richness and diversity. Children with higher SSBs consumption showed altered abundance of several oral microbiota at genus level, including increases in Streptococcus, Gemella, Neisseria,Capnocytophaga and Lautropia (and decreases in Fusobacterium, Lachnoanaerobaculum, Soonwooa, Tannerella and Moraxella (p< 0.05). Functional analysis illustrated that oral microbiota was more conducive to the pathway activated of lysine biosynthesis (p=0.045), protein export (p=0.020), D-glutamine and D-glutamate metabolism (p=0.013), as well as pantothenate and CoA biosynthesis (p=0.004) in children with higher SSBs intake. Our finding suggests that higher SSBs consumption may disturb oral microecology and reduce diversity and abundance of oral microbiota during childhood.
Figure 1
Figure 2
Figure 3