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Research article
Guanghai Qi
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1540-9525
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This work is licensed under a CC BY 4.0 License. Read Full License
Background The compromised performance of laying hens in the late phase of production relative to the peak production was thought to be associated with the impairment of intestinal functionality, which plays essential roles in contributing to their overall health and production performance. In the present study, RNA sequencing was used to investigate differences in the expression profile of intestinal functionality-related genes and associated pathways between layers in the late phase and peak phase of production.
Results A total of 104 upregulated genes with 190 downregulated genes were identified in the ileum of layers in the late phase of production compared to those at peak production. These upregulated genes were found to be enriched in little KEGG pathway, however, the downregulated genes were enriched in the pathways of PPAR signaling pathway, oxidative phosphorylation and glutathione metabolism. Besides, these downregulated genes were mapped to several GO clusters in relation to lipid metabolism, electron transport of respiratory chain, and oxidation resistance. Similarly, there were lower activities of total superoxide dismutase, glutathione S-transferase and Na+/K+-ATPase, and reductions of total antioxidant capacity and ATP level, along with an elevation in malondialdehyde content in the ileum of layers in the late phase of production as compared with those at peak production.
Conclusions The intestine of layers in the late phase of production were predominantly characterized by a disorder of lipid metabolism, concurrent with impairments of energy production and antioxidant property. This study uncovers the mechanism underlying differences between the intestinal functionality of layers in the late phase and peak phase of production, thereby providing potential targets for the genetic control or dietary modulation of intestinal hypofunction of layers in the late phase of production.
Keywords
Laying hen, Late Phase of Production, Intestinal Functionality, Transcriptome, Lipid Metabolism, Energy Generation, Oxidation Resistance
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Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Genomics.
No community comments so far
Editorial decision: Accept
On 21 Nov, 2019
Editor assigned
On 20 Nov, 2019
Submission checks complete
On 19 Nov, 2019
Editor invited
On 19 Nov, 2019
No comments provided
Editorial decision: Major revision
On 19 Nov, 2019
Editor assigned
On 15 Nov, 2019
Submission checks complete
On 14 Nov, 2019
Editor invited
On 14 Nov, 2019
No comments provided
Editorial decision: Major revision
On 07 Nov, 2019
Review #1 received
Received 04 Nov, 2019
Reviewer #2 agreed
On 20 Oct, 2019
Editor assigned
On 17 Oct, 2019
Reviewers invited
Invitations sent on 17 Oct, 2019
Reviewer #1 agreed
On 17 Oct, 2019
Submission checks complete
On 16 Oct, 2019
Editor invited
On 16 Oct, 2019
No comments provided
Editorial decision: Major revision
On 02 Oct, 2019
Review #4 received
Received 24 Sep, 2019
Review #5 received
Received 24 Sep, 2019
Reviewer #5 agreed
On 03 Sep, 2019
Reviewer #4 agreed
On 31 Aug, 2019
Review #3 received
Received 27 Aug, 2019
Review #2 received
Received 26 Aug, 2019
Review #1 received
Received 25 Aug, 2019
Reviewer #1 agreed
On 13 Aug, 2019
Reviewer #2 agreed
On 13 Aug, 2019
Reviewer #3 agreed
On 13 Aug, 2019
Reviewers invited
Invitations sent on 13 Aug, 2019
Editor assigned
On 08 Aug, 2019
Submission checks complete
On 07 Aug, 2019
Editor invited
On 07 Aug, 2019
Version 1
Posted 29 Jul, 2019
No comments provided
Editorial decision: Revise before sending to peer reviewers
On 06 Aug, 2019
Submission checks complete
On 23 Jul, 2019
Editor assigned
On 10 Jul, 2019
First submitted
On 09 Jul, 2019
Editor invited
On 09 Jul, 2019
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Subject Areas
Epigenetics & Genomics
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A new preprint design is coming!
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See the published version of this preprint at BMC Genomics.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Guanghai Qi
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1540-9525
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Genomics.
No community comments so far
Editorial decision: Accept
On 21 Nov, 2019
Editor assigned
On 20 Nov, 2019
Submission checks complete
On 19 Nov, 2019
Editor invited
On 19 Nov, 2019
No comments provided
Editorial decision: Major revision
On 19 Nov, 2019
Editor assigned
On 15 Nov, 2019
Submission checks complete
On 14 Nov, 2019
Editor invited
On 14 Nov, 2019
No comments provided
Editorial decision: Major revision
On 07 Nov, 2019
Review #1 received
Received 04 Nov, 2019
Reviewer #2 agreed
On 20 Oct, 2019
Editor assigned
On 17 Oct, 2019
Reviewers invited
Invitations sent on 17 Oct, 2019
Reviewer #1 agreed
On 17 Oct, 2019
Submission checks complete
On 16 Oct, 2019
Editor invited
On 16 Oct, 2019
No comments provided
Editorial decision: Major revision
On 02 Oct, 2019
Review #4 received
Received 24 Sep, 2019
Review #5 received
Received 24 Sep, 2019
Reviewer #5 agreed
On 03 Sep, 2019
Reviewer #4 agreed
On 31 Aug, 2019
Review #3 received
Received 27 Aug, 2019
Review #2 received
Received 26 Aug, 2019
Review #1 received
Received 25 Aug, 2019
Reviewer #1 agreed
On 13 Aug, 2019
Reviewer #2 agreed
On 13 Aug, 2019
Reviewer #3 agreed
On 13 Aug, 2019
Reviewers invited
Invitations sent on 13 Aug, 2019
Editor assigned
On 08 Aug, 2019
Submission checks complete
On 07 Aug, 2019
Editor invited
On 07 Aug, 2019
Version 1
Posted 29 Jul, 2019
No comments provided
Editorial decision: Revise before sending to peer reviewers
On 06 Aug, 2019
Submission checks complete
On 23 Jul, 2019
Editor assigned
On 10 Jul, 2019
First submitted
On 09 Jul, 2019
Editor invited
On 09 Jul, 2019
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Epigenetics & Genomics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Genomics.
Background The compromised performance of laying hens in the late phase of production relative to the peak production was thought to be associated with the impairment of intestinal functionality, which plays essential roles in contributing to their overall health and production performance. In the present study, RNA sequencing was used to investigate differences in the expression profile of intestinal functionality-related genes and associated pathways between layers in the late phase and peak phase of production.
Results A total of 104 upregulated genes with 190 downregulated genes were identified in the ileum of layers in the late phase of production compared to those at peak production. These upregulated genes were found to be enriched in little KEGG pathway, however, the downregulated genes were enriched in the pathways of PPAR signaling pathway, oxidative phosphorylation and glutathione metabolism. Besides, these downregulated genes were mapped to several GO clusters in relation to lipid metabolism, electron transport of respiratory chain, and oxidation resistance. Similarly, there were lower activities of total superoxide dismutase, glutathione S-transferase and Na+/K+-ATPase, and reductions of total antioxidant capacity and ATP level, along with an elevation in malondialdehyde content in the ileum of layers in the late phase of production as compared with those at peak production.
Conclusions The intestine of layers in the late phase of production were predominantly characterized by a disorder of lipid metabolism, concurrent with impairments of energy production and antioxidant property. This study uncovers the mechanism underlying differences between the intestinal functionality of layers in the late phase and peak phase of production, thereby providing potential targets for the genetic control or dietary modulation of intestinal hypofunction of layers in the late phase of production.
Figure 1
Figure 2
Figure 3
Figure 4
This is a list of supplementary files associated with this preprint. Click to download.
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