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Research
Cuiping Han
Corresponding Author
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Background: Development of sensitive and specific imaging approaches for the detection of ovarian cancer holds great promise for improving the therapeutic efficacy and the lifespan of the patients.
Results: In this study, manganese-nitrogen doped carbon nanosheets (Mn-N-CNSs) coupled with Anti-HE4 monoclonal antibody ([email protected]) were synthesized for the specific and targeted fluorescence/MR dual-modal imaging of ovarian carcinoma. The prepared Mn-N-CNSs revealed excellent aqueous dispersity, good colloidal stability, great optical properties and high longtudinal relaxivity rate (r1 =10.30 mM-1s-1). Encouraged by the tunable photoluminiscence of the nanoprobe and Anti-HE4 targeting ligand, the ovarian carcinoma cells were specifically labeled by the [email protected] nanoprobe with multi-color fluorescences. Benefiting from the high r1 relaxivity, the nanoprobe exhibited targeted and enhanced MR contrast effect in the ovarian carcinoma cells and tumor bearing mice model. Besides, the high biocompatibility and easy excretion from the body of the nanoprobe were further confirmed in vivo.
Conclusion: The prepared [email protected] with excellent biocompatibility, high-performance and superior tumor-targeting ability provides a novel fluorescence/MR dual-modal nanoprobe for specific labeling and detection of ovarian carcinoma cells in vitro and in vivo.
Keywords
Manganese, Carbon nanosheets, Tumor targeting, Fluorescence/MR dual-modal nanoprobe
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Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of Nanobiotechnology.
Version 2
Posted 14 Nov, 2020
No community comments so far
Editorial decision: Accept
On 17 Nov, 2020
Review #2 received
Received 16 Nov, 2020
Reviewer #2 agreed
On 15 Nov, 2020
Review #1 received
Received 12 Nov, 2020
Reviewer #1 agreed
On 06 Nov, 2020
Reviewers invited
Invitations sent on 05 Nov, 2020
Editor assigned
On 04 Nov, 2020
Submission checks complete
On 04 Nov, 2020
Editor invited
On 04 Nov, 2020
No comments provided
Review #1 received
Received 24 Sep, 2020
Editorial decision: Major revision
On 24 Sep, 2020
Review #2 received
Received 20 Sep, 2020
Reviewer #2 agreed
On 31 May, 2020
Reviewer #1 agreed
On 17 May, 2020
Reviewers invited
Invitations sent on 16 May, 2020
Editor invited
On 12 May, 2020
Editor assigned
On 12 May, 2020
First submitted
On 11 May, 2020
Submission checks complete
On 11 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Nanoscience
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A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
See the published version of this preprint at Journal of Nanobiotechnology.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Cuiping Han
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at Journal of Nanobiotechnology.
Version 2
Posted 14 Nov, 2020
No community comments so far
Editorial decision: Accept
On 17 Nov, 2020
Review #2 received
Received 16 Nov, 2020
Reviewer #2 agreed
On 15 Nov, 2020
Review #1 received
Received 12 Nov, 2020
Reviewer #1 agreed
On 06 Nov, 2020
Reviewers invited
Invitations sent on 05 Nov, 2020
Editor assigned
On 04 Nov, 2020
Submission checks complete
On 04 Nov, 2020
Editor invited
On 04 Nov, 2020
No comments provided
Review #1 received
Received 24 Sep, 2020
Editorial decision: Major revision
On 24 Sep, 2020
Review #2 received
Received 20 Sep, 2020
Reviewer #2 agreed
On 31 May, 2020
Reviewer #1 agreed
On 17 May, 2020
Reviewers invited
Invitations sent on 16 May, 2020
Editor invited
On 12 May, 2020
Editor assigned
On 12 May, 2020
First submitted
On 11 May, 2020
Submission checks complete
On 11 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Nanoscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of Nanobiotechnology.
Background: Development of sensitive and specific imaging approaches for the detection of ovarian cancer holds great promise for improving the therapeutic efficacy and the lifespan of the patients.
Results: In this study, manganese-nitrogen doped carbon nanosheets (Mn-N-CNSs) coupled with Anti-HE4 monoclonal antibody ([email protected]) were synthesized for the specific and targeted fluorescence/MR dual-modal imaging of ovarian carcinoma. The prepared Mn-N-CNSs revealed excellent aqueous dispersity, good colloidal stability, great optical properties and high longtudinal relaxivity rate (r1 =10.30 mM-1s-1). Encouraged by the tunable photoluminiscence of the nanoprobe and Anti-HE4 targeting ligand, the ovarian carcinoma cells were specifically labeled by the [email protected] nanoprobe with multi-color fluorescences. Benefiting from the high r1 relaxivity, the nanoprobe exhibited targeted and enhanced MR contrast effect in the ovarian carcinoma cells and tumor bearing mice model. Besides, the high biocompatibility and easy excretion from the body of the nanoprobe were further confirmed in vivo.
Conclusion: The prepared [email protected] with excellent biocompatibility, high-performance and superior tumor-targeting ability provides a novel fluorescence/MR dual-modal nanoprobe for specific labeling and detection of ovarian carcinoma cells in vitro and in vivo.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
This is a list of supplementary files associated with this preprint. Click to download.
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