This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Femi Ayoade
African Centre of Excellence for the Genomics of Infectious Diseases (ACEGID)
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Shiga toxigenic strains of E. coli (STEC) known to be etiological agents for diarrhea were screened for their incidence/ occurrence in selected abattoirs and retail meat sources in Osogbo metropolis of Osun State, Nigeria using a randomized block design. Samples were plated directly on selective and differential media and confirmed serologically using latex agglutination serotyping kit, then, multiplex PCR analysis was used to screen for the presence of specific virulence factors. The results showed a percent occurrence of STEC at the sampled sites ranging from 25.8–46.3%. None of the strains showed any visible agglutination with the O157 latex reagent. Sequence analysis of PCR products was performed on a representative isolate showing the highest combination of virulence genes. This sequence was subsequently submitted to GenBank with accession number MW463885. The sequence showed 100% coverage and 96.46% percentage identity with Escherichia coli O113:H21 (GenBank Accession number: CP031892.1) strain from Canada. From evolutionary analyses, the strain from Nigeria, sequenced in this study, is evolutionarily distant when compared with the publicly available sequences from Nigeria. Although no case of E. coli O157 was found within the study area, percent occurrence of non-O157 STEC as high as 46.3% at some of the sampled sites is worrisome and requires regulatory interventions in ensuring hygienic practices at the abattoirs within the study area.
Keywords
E. coli, STEC, abattoirs
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 12 Mar, 2021
No community comments so far
Review #1 received
Received 30 Mar, 2021
Reviewer #2 agreed
On 15 Mar, 2021
Reviewer #4 agreed
On 15 Mar, 2021
Reviewer #3 agreed
On 15 Mar, 2021
Reviewer #5 agreed
On 15 Mar, 2021
Reviewer #1 agreed
On 15 Mar, 2021
Reviewers invited
Invitations sent on 12 Mar, 2021
Editor assigned
On 12 Mar, 2021
Editor invited
On 12 Mar, 2021
Submission checks complete
On 11 Mar, 2021
First submitted
On 28 Feb, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Learn more about our company.
This preprint is under consideration at Scientific Reports. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Femi Ayoade
African Centre of Excellence for the Genomics of Infectious Diseases (ACEGID)
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 12 Mar, 2021
No community comments so far
Review #1 received
Received 30 Mar, 2021
Reviewer #2 agreed
On 15 Mar, 2021
Reviewer #4 agreed
On 15 Mar, 2021
Reviewer #3 agreed
On 15 Mar, 2021
Reviewer #5 agreed
On 15 Mar, 2021
Reviewer #1 agreed
On 15 Mar, 2021
Reviewers invited
Invitations sent on 12 Mar, 2021
Editor assigned
On 12 Mar, 2021
Editor invited
On 12 Mar, 2021
Submission checks complete
On 11 Mar, 2021
First submitted
On 28 Feb, 2021
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Shiga toxigenic strains of E. coli (STEC) known to be etiological agents for diarrhea were screened for their incidence/ occurrence in selected abattoirs and retail meat sources in Osogbo metropolis of Osun State, Nigeria using a randomized block design. Samples were plated directly on selective and differential media and confirmed serologically using latex agglutination serotyping kit, then, multiplex PCR analysis was used to screen for the presence of specific virulence factors. The results showed a percent occurrence of STEC at the sampled sites ranging from 25.8–46.3%. None of the strains showed any visible agglutination with the O157 latex reagent. Sequence analysis of PCR products was performed on a representative isolate showing the highest combination of virulence genes. This sequence was subsequently submitted to GenBank with accession number MW463885. The sequence showed 100% coverage and 96.46% percentage identity with Escherichia coli O113:H21 (GenBank Accession number: CP031892.1) strain from Canada. From evolutionary analyses, the strain from Nigeria, sequenced in this study, is evolutionarily distant when compared with the publicly available sequences from Nigeria. Although no case of E. coli O157 was found within the study area, percent occurrence of non-O157 STEC as high as 46.3% at some of the sampled sites is worrisome and requires regulatory interventions in ensuring hygienic practices at the abattoirs within the study area.
Figure 1
Figure 2
Loading...