Early diagnosis of ocular diseases improves the understanding of pathophysiology and aids in accurate monitoring and effective treatment. Advanced, multimodal ocular imaging platforms play a crucial role in visualization of ocular components and provide clinicians with a valuable tool for evaluating various eye diseases. Here, for the first time we present a non-contact, multiwavelength photoacoustic remote sensing (PARS) microscopy and swept-source optical coherence tomography (SS-OCT) for in-vivo functional and structural imaging of the eye. The system provides complementary imaging contrasts of optical absorption and optical scattering, and is used for non-contact, in-vivo imaging of murine eye. Results of vasculature and structural imaging as well as melanin content in the retinal pigment epithelium layer are presented. Multiwavelength PARS microscopy using Stimulated Raman Scattering is applied to enable in-vivo, non-contact oxygen saturation estimation in the ocular tissue. The reported work may be a major step towards clinical translation of ophthalmic technologies and has the potential to advance the diagnosis and treatment of ocular diseases.
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Competing interest reported. Author P. Haji Reza has financial interests in illumiSonics Inc. IllumiSonics partially supported this work. All other authors have no competing/conflict of interest.
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Posted 15 Mar, 2021
On 24 Mar, 2021
Received 14 Mar, 2021
Received 14 Mar, 2021
Received 14 Mar, 2021
Received 14 Mar, 2021
Received 14 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
Invitations sent on 06 Mar, 2021
On 04 Mar, 2021
On 04 Mar, 2021
On 03 Mar, 2021
On 01 Mar, 2021
Posted 15 Mar, 2021
On 24 Mar, 2021
Received 14 Mar, 2021
Received 14 Mar, 2021
Received 14 Mar, 2021
Received 14 Mar, 2021
Received 14 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
On 06 Mar, 2021
Invitations sent on 06 Mar, 2021
On 04 Mar, 2021
On 04 Mar, 2021
On 03 Mar, 2021
On 01 Mar, 2021
Early diagnosis of ocular diseases improves the understanding of pathophysiology and aids in accurate monitoring and effective treatment. Advanced, multimodal ocular imaging platforms play a crucial role in visualization of ocular components and provide clinicians with a valuable tool for evaluating various eye diseases. Here, for the first time we present a non-contact, multiwavelength photoacoustic remote sensing (PARS) microscopy and swept-source optical coherence tomography (SS-OCT) for in-vivo functional and structural imaging of the eye. The system provides complementary imaging contrasts of optical absorption and optical scattering, and is used for non-contact, in-vivo imaging of murine eye. Results of vasculature and structural imaging as well as melanin content in the retinal pigment epithelium layer are presented. Multiwavelength PARS microscopy using Stimulated Raman Scattering is applied to enable in-vivo, non-contact oxygen saturation estimation in the ocular tissue. The reported work may be a major step towards clinical translation of ophthalmic technologies and has the potential to advance the diagnosis and treatment of ocular diseases.
Figure 1
Figure 2
Figure 3
Figure 4
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