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Research
A fraction of Prunella vulgaris spike extract inhibited neutrophil elastase and protected mice against lipopolysaccharide-induced acute lung injury
Pei-Wen Hsieh
Chang Gung University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8882-4711
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This work is licensed under a CC BY 4.0 License. Read Full License
Background
Human neutrophil elastase (HNE) is an abundantly expressed neutrophil serine protease that promotes neutrophil invasion and neutrophil extracellular trap (NET) formation, thereby mediating lung tissue destruction and enhancing pulmonary inflammation in acute lung injury (ALI). Chemical agents that target HNE and manipulate HNE level homeostasis are desired to prevent or treat ALI.
Methods
In a search for HNE inhibitors, traditional Chinese medicines were evaluated, leading to the identification of the water extract of spikes of Prunella vulgaris as an inhibitor of HNE activity. Using bioactivity-guided fractionation, a bioactive fraction (PVAP) that inhibited NHE activity was prepared, and its effects on lipopolysaccharide (LPS)-induced ALI were studied.
Results
The results suggested that PVAP elicited protective effects against LPS-induced ALI by inhibiting HNE activity, thereby reducing neutrophil invasion, pro-inflammatory cytokine release, and NET formation.
Conclusions
These findings illustrated the utility of PVAP as an agent for preventing ALI.
Keywords
Neutrophil elastase, Acute lung injury, Prunella vulgaris, Traditional Chinese medicines, Bioactivity-guided fractionation
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This is a preprint. It has not completed peer review.
Research
A fraction of Prunella vulgaris spike extract inhibited neutrophil elastase and protected mice against lipopolysaccharide-induced acute lung injury
Pei-Wen Hsieh
Chang Gung University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-8882-4711
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 21 May, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Pulmonology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Human neutrophil elastase (HNE) is an abundantly expressed neutrophil serine protease that promotes neutrophil invasion and neutrophil extracellular trap (NET) formation, thereby mediating lung tissue destruction and enhancing pulmonary inflammation in acute lung injury (ALI). Chemical agents that target HNE and manipulate HNE level homeostasis are desired to prevent or treat ALI.
Methods
In a search for HNE inhibitors, traditional Chinese medicines were evaluated, leading to the identification of the water extract of spikes of Prunella vulgaris as an inhibitor of HNE activity. Using bioactivity-guided fractionation, a bioactive fraction (PVAP) that inhibited NHE activity was prepared, and its effects on lipopolysaccharide (LPS)-induced ALI were studied.
Results
The results suggested that PVAP elicited protective effects against LPS-induced ALI by inhibiting HNE activity, thereby reducing neutrophil invasion, pro-inflammatory cytokine release, and NET formation.
Conclusions
These findings illustrated the utility of PVAP as an agent for preventing ALI.
Figure 1
Figure 2
Figure 3