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Background: Bladder cancer (BCa) is the most common cancer in the urinary system with high recurrence rate and poor prognosis. Circular RNA (circRNA) is a novel subclass of noncoding-RNA
which participate in progression of BCa. Here, we identified a novel circRNA—circAPP and aimed to investigate the role of circAPP in progression of BCa.
Method: Public data of RNA sequencing was used to identify significant circRNA related to BCa. The role of circRNAs in progression in BCa was assessed in cytotoxicity assay, transwell assay and
flow cytometry. Biotin-coupled RNA pull-down and fluorescence in situ hybridization (FISH) were performed to evaluate the interaction between circRNAs and miRNAs.
Results: The expression of circAPP was higher in BCa tissues and cells than in normal samples. In vitro experiments showed that knockdown of circAPP inhibited cell proliferation and impeded the metastasis of BCa cells. Mechanistically, we demonstrated that circAPP acts as a sponge for miR-186-5p and promotes host gene APP’s expression. Clinically, circAPP predicts worse overall survival of BCa patients, indicating its prognostic value.
Conclusion: Our study identified that circAPP modulates metastasis of BCa through miR-186-5p/APP aixs and may serve as a promising prognostic biomarker for BCa, which provides novel insights into treatment of BCa.
Keywords
bladder cancer, malignant progression, miRNA-186, circAPP
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This is a preprint. It has not completed peer review.
Primary research
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 12 Apr, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: Bladder cancer (BCa) is the most common cancer in the urinary system with high recurrence rate and poor prognosis. Circular RNA (circRNA) is a novel subclass of noncoding-RNA
which participate in progression of BCa. Here, we identified a novel circRNA—circAPP and aimed to investigate the role of circAPP in progression of BCa.
Method: Public data of RNA sequencing was used to identify significant circRNA related to BCa. The role of circRNAs in progression in BCa was assessed in cytotoxicity assay, transwell assay and
flow cytometry. Biotin-coupled RNA pull-down and fluorescence in situ hybridization (FISH) were performed to evaluate the interaction between circRNAs and miRNAs.
Results: The expression of circAPP was higher in BCa tissues and cells than in normal samples. In vitro experiments showed that knockdown of circAPP inhibited cell proliferation and impeded the metastasis of BCa cells. Mechanistically, we demonstrated that circAPP acts as a sponge for miR-186-5p and promotes host gene APP’s expression. Clinically, circAPP predicts worse overall survival of BCa patients, indicating its prognostic value.
Conclusion: Our study identified that circAPP modulates metastasis of BCa through miR-186-5p/APP aixs and may serve as a promising prognostic biomarker for BCa, which provides novel insights into treatment of BCa.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
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