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Research Article
Marina Mauro Gomes
University of Sao Paulo: Universidade de Sao Paulo
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2263-8681
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The hydrothermolysis variables temperature (150 - 210 °C) and time (10 - 20 minutes) were assessed to improve hydrolysis efficiency of brewery spent grain (BSG) for renewable energy generation. The intensification of the pretreatment was expressed by the severity variation (2.8 - 4.5) and the process was optimized with methane production of 411.6 ± 7.2 mL. g -1 STV (severity 4.2). The fermentation-methanogenesis of BSG and hydrolysate resulting from BSG hydrothermolysis process under severity of 4.2 (210 o C for 10 min.) was evaluated by central composite design (CCD) with the variables operation temperature (30 - 60 °C), BSG concentration (7.3 - 20.7 g.L -1 ) and hydrolysate (0 - 12.4 mL). The higher methane production observed was 305.8 mL.g -1 STV, with 14 g.L -1 of BSG, without hydrolysate at 45 °C. The main soluble metabolites were acetic acid (233.17 mg.L -1 ) and butyric acid (156.0 mg.L -1 ). On other hand, the lower methane production (108.5 ± 2.0 mL. g -1 STV) verified was 14 g.L -1 of BSG, 6.5 mL of hydrolysate at 60 °C, which revealed that in this condition propionic acid (947.4 mg.L -1 ) and acetic acid (599.2 mg.L -1 ) were expressive. In the optimal fermentation-methanogenic condition of pretreated BSG, Macellibacteroides and Sphaerochaeta (15.9 and 14.7%, respectively) were identified, as well as archaea similar to Methanosaeta (80.4%), favoring the acetoclastic methanogenic pathway.
Keywords
Acetoclastic methanogenesis, Hydrolysate, Lignocellulosic biomass, Pretreatment severity, Response surface methodology.
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This preprint is under consideration at Waste and Biomass Valorization. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research Article
Marina Mauro Gomes
University of Sao Paulo: Universidade de Sao Paulo
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2263-8681
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 19 Mar, 2021
No community comments so far
Review #? received
Received 16 Mar, 2021
Reviewers invited
Invitations sent on 16 Mar, 2021
Editor invited
On 14 Mar, 2021
Editor assigned
On 03 Mar, 2021
First submitted
On 02 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biomaterials
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The hydrothermolysis variables temperature (150 - 210 °C) and time (10 - 20 minutes) were assessed to improve hydrolysis efficiency of brewery spent grain (BSG) for renewable energy generation. The intensification of the pretreatment was expressed by the severity variation (2.8 - 4.5) and the process was optimized with methane production of 411.6 ± 7.2 mL. g -1 STV (severity 4.2). The fermentation-methanogenesis of BSG and hydrolysate resulting from BSG hydrothermolysis process under severity of 4.2 (210 o C for 10 min.) was evaluated by central composite design (CCD) with the variables operation temperature (30 - 60 °C), BSG concentration (7.3 - 20.7 g.L -1 ) and hydrolysate (0 - 12.4 mL). The higher methane production observed was 305.8 mL.g -1 STV, with 14 g.L -1 of BSG, without hydrolysate at 45 °C. The main soluble metabolites were acetic acid (233.17 mg.L -1 ) and butyric acid (156.0 mg.L -1 ). On other hand, the lower methane production (108.5 ± 2.0 mL. g -1 STV) verified was 14 g.L -1 of BSG, 6.5 mL of hydrolysate at 60 °C, which revealed that in this condition propionic acid (947.4 mg.L -1 ) and acetic acid (599.2 mg.L -1 ) were expressive. In the optimal fermentation-methanogenic condition of pretreated BSG, Macellibacteroides and Sphaerochaeta (15.9 and 14.7%, respectively) were identified, as well as archaea similar to Methanosaeta (80.4%), favoring the acetoclastic methanogenic pathway.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
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