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Research Article
Shahzad Bhatti
Lahore Institute of Fertility and Endocrinology, Hameed Latif Hospital, New Garden Town, Lahore-54800 Pakistan.
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Declarations:
Declarations
- This study involved human subjects.
- The author confirmed that all appropriate ethical guidelines for the use of human subjects have been followed, any necessary IRB and/or ethics committee review has been obtained, and information about the IRB/ethics committee is included in the manuscript.
- The author has confirmed that all necessary patient/participant consent or assent has been obtained and the appropriate institutional forms have been archived. If the IRB/ethics committee waived the requirement for patient/participant consent or assent, an explanation for the waiver is included in the text.
- This study involved the use of non-human vertebrates.
- The author has confirmed that all appropriate ethical guidelines for the handling and use of animals in research have been followed and details of the oversight board have been included in the text.
- The author has confirmed that a statement listing potential conflicts of interest or lack thereof is included in the text.
This retrospective case control study was executed to compare the efficacy of short and long acting Gonadotropin releasing hormone agonist (GnRH-a) on pregnancy outcome. A total of 540 cycles with a history of male factor were identified from 2013 to 2016. The patients were separated into two groups: long acting protocol (LAP) group consisting of 310 cycles which were induced by a combination of GnRH-a incorporated with Follicular stimulating hormone (FSH) and Human menopausal gonadotropin (hMG), while short acting protocol (SAP) group or flare up regimen comprises of 230 cycles introduced by a combination of GnRH-a along with FSH and hMG. Patients are divided into subsequent age groups ≤ 30, 31–35, 36–40 and > 40 years. In comparison with the SAP group, the duration of ovarian stimulation, calculated dose of GnRH-a, number of retrieved mature oocytes, good quality embryos for implantation and persistent pregnancies were all significantly found to be high (p < 0.05) in the LAP group as compared to the SAP group. The clinical pregnancy rates were comparatively high in the LAP group (33.12%; P = 0.001) than in the SAP group (28.23%). The findings of our study revealed that irrespective of patient’s age, the long acting protocol was more reliable and fertile with reference to number of mature oocytes retrieved, time period for stimulation, total dose of GnRH-a during controlled ovarian hyperstimulation, high quality embryos, fertilization and cleavage rate as well as pregnancy outcome.
Keywords
short and long GnRH-a protocols, In vitro fertilization, pituitary desensitization, Intracytoplasmic sperm injection, Pregnancy outcome
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This is a preprint. It has not completed peer review.
Research Article
Shahzad Bhatti
Lahore Institute of Fertility and Endocrinology, Hameed Latif Hospital, New Garden Town, Lahore-54800 Pakistan.
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 04 Mar, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Developmental Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Declarations:
Declarations
- This study involved human subjects.
- The author confirmed that all appropriate ethical guidelines for the use of human subjects have been followed, any necessary IRB and/or ethics committee review has been obtained, and information about the IRB/ethics committee is included in the manuscript.
- The author has confirmed that all necessary patient/participant consent or assent has been obtained and the appropriate institutional forms have been archived. If the IRB/ethics committee waived the requirement for patient/participant consent or assent, an explanation for the waiver is included in the text.
- This study involved the use of non-human vertebrates.
- The author has confirmed that all appropriate ethical guidelines for the handling and use of animals in research have been followed and details of the oversight board have been included in the text.
- The author has confirmed that a statement listing potential conflicts of interest or lack thereof is included in the text.
This retrospective case control study was executed to compare the efficacy of short and long acting Gonadotropin releasing hormone agonist (GnRH-a) on pregnancy outcome. A total of 540 cycles with a history of male factor were identified from 2013 to 2016. The patients were separated into two groups: long acting protocol (LAP) group consisting of 310 cycles which were induced by a combination of GnRH-a incorporated with Follicular stimulating hormone (FSH) and Human menopausal gonadotropin (hMG), while short acting protocol (SAP) group or flare up regimen comprises of 230 cycles introduced by a combination of GnRH-a along with FSH and hMG. Patients are divided into subsequent age groups ≤ 30, 31–35, 36–40 and > 40 years. In comparison with the SAP group, the duration of ovarian stimulation, calculated dose of GnRH-a, number of retrieved mature oocytes, good quality embryos for implantation and persistent pregnancies were all significantly found to be high (p < 0.05) in the LAP group as compared to the SAP group. The clinical pregnancy rates were comparatively high in the LAP group (33.12%; P = 0.001) than in the SAP group (28.23%). The findings of our study revealed that irrespective of patient’s age, the long acting protocol was more reliable and fertile with reference to number of mature oocytes retrieved, time period for stimulation, total dose of GnRH-a during controlled ovarian hyperstimulation, high quality embryos, fertilization and cleavage rate as well as pregnancy outcome.
Figure 1
Figure 2
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