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Research
YoungJoon Seo
Yonsei University - Wonju Campus
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2839-4676
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, although stem cells of different origins in humans have similar payloads.
Results
Here, the payload of EVs released by crosstalk between MSCs and human middle ear epithelial cells (HMEECs) extracted from adipose tissue, bone marrow and tonsils significantly increased the level of anti-inflammatory factors. EVs derived from the co-culture medium decreased TNF-α, COX-2, IL-1β, and IL-6 levels to approximately zero within 3 h in HMEECs. Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively.
Conclusions
In conclusion, stem cells of different origins have different payloads through crosstalk with recipient-specific cells. Inducing specific factors in EVs by co-culture with MSCs could be valuable in regenerative medicine.
Keywords
Extracellular vesicles, mesenchymal stem cell, antiinflammation, amyloid-β A4 precursor protein-binding family A member 2, miR-638
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Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 16 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 05 Apr, 2021
Review #2 received
Received 03 Apr, 2021
Review #1 received
Received 29 Mar, 2021
Reviews received
Received 17 Mar, 2021
Reviewer #2 agreed
On 16 Mar, 2021
Reviewer #1 agreed
On 16 Mar, 2021
Reviewers invited
Invitations sent on 07 Mar, 2021
Editor assigned
On 05 Mar, 2021
Editor invited
On 04 Mar, 2021
Submission checks complete
On 04 Mar, 2021
First submitted
On 02 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Nanoscience
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A new preprint design is coming!
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This preprint is under consideration at Journal of Nanobiotechnology. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
YoungJoon Seo
Yonsei University - Wonju Campus
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2839-4676
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 16 Mar, 2021
No community comments so far
Editorial decision: Major revision
On 05 Apr, 2021
Review #2 received
Received 03 Apr, 2021
Review #1 received
Received 29 Mar, 2021
Reviews received
Received 17 Mar, 2021
Reviewer #2 agreed
On 16 Mar, 2021
Reviewer #1 agreed
On 16 Mar, 2021
Reviewers invited
Invitations sent on 07 Mar, 2021
Editor assigned
On 05 Mar, 2021
Editor invited
On 04 Mar, 2021
Submission checks complete
On 04 Mar, 2021
First submitted
On 02 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Nanoscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
The application of extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) requires customized materials to target disease or cell damage. We hypothesized that EVs exert different inflammatory effects on one recipient cell, although stem cells of different origins in humans have similar payloads.
Results
Here, the payload of EVs released by crosstalk between MSCs and human middle ear epithelial cells (HMEECs) extracted from adipose tissue, bone marrow and tonsils significantly increased the level of anti-inflammatory factors. EVs derived from the co-culture medium decreased TNF-α, COX-2, IL-1β, and IL-6 levels to approximately zero within 3 h in HMEECs. Expression of miR-638 and amyloid-β A4 precursor protein-binding family A member 2 was analyzed using microarrays and gene ontology analysis, respectively.
Conclusions
In conclusion, stem cells of different origins have different payloads through crosstalk with recipient-specific cells. Inducing specific factors in EVs by co-culture with MSCs could be valuable in regenerative medicine.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
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