This preprint is under consideration at Stem Cell Research & Therapy. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
Research
Hon-Kan Yip
Chang Gung Memorial Hospital Kaohsiung Branch
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6305-5717
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: This study tested the hypothesis that combined melatonin (Mel) and adipose-derived mesenchymal stem cell (ADMSC) treatment was superior to either one alone on protecting the testis against acute testicular torsion-induced ischemia-reperfusion (TTIR) injury.
Methods and Results: Male-adult SD rats (n=30) were equally categorized into group 1 (sham-operated control), group 2 [TTIR/by torsion of right/left testis (i.e., ischemia) with rotated 720 degrees counterclockwisely for 2h, then detorsion (i.e., reperfusion) to the original position for 72h], group 3 (TTIR + Mel/intraperitoneal administration/50 mg/kg at 30 minutes after ischemia, followed by 20 mg at 3h and days 1/2/3 after TTIR), group 4 (TTIR + ADMSC/1.2 x 106 cells/intravenous administration at 30 minutes after ischemia, followed by days 1/2 TTIR) and group 5 (TTIR + Mel + ADMSC). The result showed that the protein expressions of oxidative-stress (NOX-1/NOX-2/oxidized-protein), apoptotic/mitochondrial-damaged (mitochondrial-Bax/cleaved-caspase3/cleaved-PARP/cytosolic-cytochrome C) and fibrotic (TGF-ß/Smad3) biomarkers as well as testicular damage scores were lowest in group 1, highest in group 2 and significantly higher in groups 3/4 than in group 5, but they showed no difference between groups 3/4, whereas the protein expressions of androgen receptor (AR) and vimentin showed an opposite pattern of oxidative stress (all p<0.0001). The cellular levels of inflammation (MMP-9/MPO/CD68) exhibited an identical pattern, whereas the numbers of Sertoli cells, α-tubulin, AR and vimentin as well as thickness of seminiferous tubule exhibited an opposite pattern of oxidative stress among the groups (all p<0.0001).
Conclusion: Mel-ADMSCs effectively protected the testis against TTIR injury.
Keywords
testis, ischemia-reperfusion injury, melatonin, adipose-derived mesenchymal stem cells
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Loading...
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 12 Mar, 2021
No community comments so far
Editorial decision: Major Revision
On 01 May, 2021
Review #2 received
Received 30 Apr, 2021
Reviewer #2 agreed
On 28 Apr, 2021
Review #1 received
Received 22 Mar, 2021
Reviews received
Received 13 Mar, 2021
Reviewer #1 agreed
On 13 Mar, 2021
Editor assigned
On 05 Mar, 2021
Reviewers invited
Invitations sent on 05 Mar, 2021
Editor invited
On 05 Mar, 2021
Submission checks complete
On 05 Mar, 2021
First submitted
On 03 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Learn more about our company.
A new preprint design is coming!
We have improved readability, clarity, accessibility, and opportunities for engagement.
This preprint is under consideration at Stem Cell Research & Therapy. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Hon-Kan Yip
Chang Gung Memorial Hospital Kaohsiung Branch
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6305-5717
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 12 Mar, 2021
No community comments so far
Editorial decision: Major Revision
On 01 May, 2021
Review #2 received
Received 30 Apr, 2021
Reviewer #2 agreed
On 28 Apr, 2021
Review #1 received
Received 22 Mar, 2021
Reviews received
Received 13 Mar, 2021
Reviewer #1 agreed
On 13 Mar, 2021
Editor assigned
On 05 Mar, 2021
Reviewers invited
Invitations sent on 05 Mar, 2021
Editor invited
On 05 Mar, 2021
Submission checks complete
On 05 Mar, 2021
First submitted
On 03 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background: This study tested the hypothesis that combined melatonin (Mel) and adipose-derived mesenchymal stem cell (ADMSC) treatment was superior to either one alone on protecting the testis against acute testicular torsion-induced ischemia-reperfusion (TTIR) injury.
Methods and Results: Male-adult SD rats (n=30) were equally categorized into group 1 (sham-operated control), group 2 [TTIR/by torsion of right/left testis (i.e., ischemia) with rotated 720 degrees counterclockwisely for 2h, then detorsion (i.e., reperfusion) to the original position for 72h], group 3 (TTIR + Mel/intraperitoneal administration/50 mg/kg at 30 minutes after ischemia, followed by 20 mg at 3h and days 1/2/3 after TTIR), group 4 (TTIR + ADMSC/1.2 x 106 cells/intravenous administration at 30 minutes after ischemia, followed by days 1/2 TTIR) and group 5 (TTIR + Mel + ADMSC). The result showed that the protein expressions of oxidative-stress (NOX-1/NOX-2/oxidized-protein), apoptotic/mitochondrial-damaged (mitochondrial-Bax/cleaved-caspase3/cleaved-PARP/cytosolic-cytochrome C) and fibrotic (TGF-ß/Smad3) biomarkers as well as testicular damage scores were lowest in group 1, highest in group 2 and significantly higher in groups 3/4 than in group 5, but they showed no difference between groups 3/4, whereas the protein expressions of androgen receptor (AR) and vimentin showed an opposite pattern of oxidative stress (all p<0.0001). The cellular levels of inflammation (MMP-9/MPO/CD68) exhibited an identical pattern, whereas the numbers of Sertoli cells, α-tubulin, AR and vimentin as well as thickness of seminiferous tubule exhibited an opposite pattern of oxidative stress among the groups (all p<0.0001).
Conclusion: Mel-ADMSCs effectively protected the testis against TTIR injury.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Loading...