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Lee-Yuan Liu-Chen
Lewis Katz School of Medicine at Temple University
Corresponding Author
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Objective: Selective kappa opioid receptor (KOR) agonists were shown to produce a dose-dependent depression of brain-stimulation reward (BSR) in the rat intracranial self-stimulation (ICSS) tests. However, limited studies using mice produced less conclusive results. Here the effects of U50,488H were re-examined on BSR in mice with a larger cohort of animals.
Results: Forty C57BL/6J male mice were implanted with the electrodes in medial forebrain bundle. About a week after surgery, mice were subject to ICSS training. Only eighteen passed the two-phase procedures, at which point they readily spun the wheels to obtain reinforcing effect of BSR, and were used for the ICSS tests. Compared with saline (s.c.), U50,488H (2 mg/kg, s.c.) did not have effects on the BSR thresholds within 1 h post-treatment, while it decreased the maximum wheel-spinning rates in a time-dependent manner. In contrast, cocaine (5 mg/kg, s.c.) decreased the BSR thresholds time-dependently without affecting the maximum wheel-spinning rates in the same cohort of mice, demonstrating the validity of our mouse ICSS models. For comparison, U50,488H (2 mg/kg, s.c.) induced significant conditioned place aversion (CPA) in a different cohort of mice without surgeries. Thus, ICSS may not be an appropriate test for KOR agonist-induced aversion in mice.
Keywords
kappa opioid receptor, U50,488, brain-stimulation reward, ICSS, conditioned place aversion
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CURRENT STATUS: Published
View the published article at BMC Research Notes.
Version 3
Posted 12 Aug, 2020
No community comments so far
Submission checks complete
On 06 Aug, 2020
Editorial decision: Accept
On 06 Aug, 2020
No comments provided
Editorial decision: Minor revision
On 31 Jul, 2020
Review #2 received
Received 29 Jul, 2020
Reviewer #2 agreed
On 23 Jul, 2020
Reviewers invited
Invitations sent on 21 Jul, 2020
Reviewer #1 agreed
On 21 Jul, 2020
Review #1 received
Received 21 Jul, 2020
Editor assigned
On 20 Jul, 2020
Submission checks complete
On 19 Jul, 2020
Editor invited
On 19 Jul, 2020
No comments provided
Editorial decision: Minor revision
On 12 Jul, 2020
Review #1 received
Received 10 Jul, 2020
Review #2 received
Received 18 Jun, 2020
Reviewer #2 agreed
On 15 Jun, 2020
Reviewer #1 agreed
On 12 Jun, 2020
Editor assigned
On 01 Jun, 2020
Reviewers invited
Invitations sent on 01 Jun, 2020
Submission checks complete
On 26 May, 2020
Editor invited
On 26 May, 2020
Metrics
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Subject Areas
Cellular & Molecular Neuroscience
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A new preprint design is coming!
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See the published version of this preprint at BMC Research Notes.
This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research note
Lee-Yuan Liu-Chen
Lewis Katz School of Medicine at Temple University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
View the published article at BMC Research Notes.
Version 3
Posted 12 Aug, 2020
No community comments so far
Submission checks complete
On 06 Aug, 2020
Editorial decision: Accept
On 06 Aug, 2020
No comments provided
Editorial decision: Minor revision
On 31 Jul, 2020
Review #2 received
Received 29 Jul, 2020
Reviewer #2 agreed
On 23 Jul, 2020
Reviewers invited
Invitations sent on 21 Jul, 2020
Reviewer #1 agreed
On 21 Jul, 2020
Review #1 received
Received 21 Jul, 2020
Editor assigned
On 20 Jul, 2020
Submission checks complete
On 19 Jul, 2020
Editor invited
On 19 Jul, 2020
No comments provided
Editorial decision: Minor revision
On 12 Jul, 2020
Review #1 received
Received 10 Jul, 2020
Review #2 received
Received 18 Jun, 2020
Reviewer #2 agreed
On 15 Jun, 2020
Reviewer #1 agreed
On 12 Jun, 2020
Editor assigned
On 01 Jun, 2020
Reviewers invited
Invitations sent on 01 Jun, 2020
Submission checks complete
On 26 May, 2020
Editor invited
On 26 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Cellular & Molecular Neuroscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at BMC Research Notes.
Objective: Selective kappa opioid receptor (KOR) agonists were shown to produce a dose-dependent depression of brain-stimulation reward (BSR) in the rat intracranial self-stimulation (ICSS) tests. However, limited studies using mice produced less conclusive results. Here the effects of U50,488H were re-examined on BSR in mice with a larger cohort of animals.
Results: Forty C57BL/6J male mice were implanted with the electrodes in medial forebrain bundle. About a week after surgery, mice were subject to ICSS training. Only eighteen passed the two-phase procedures, at which point they readily spun the wheels to obtain reinforcing effect of BSR, and were used for the ICSS tests. Compared with saline (s.c.), U50,488H (2 mg/kg, s.c.) did not have effects on the BSR thresholds within 1 h post-treatment, while it decreased the maximum wheel-spinning rates in a time-dependent manner. In contrast, cocaine (5 mg/kg, s.c.) decreased the BSR thresholds time-dependently without affecting the maximum wheel-spinning rates in the same cohort of mice, demonstrating the validity of our mouse ICSS models. For comparison, U50,488H (2 mg/kg, s.c.) induced significant conditioned place aversion (CPA) in a different cohort of mice without surgeries. Thus, ICSS may not be an appropriate test for KOR agonist-induced aversion in mice.
Figure 1
Figure 2
Figure 3
This is a list of supplementary files associated with this preprint. Click to download.
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