Dengue fever is a very common viral disease in Pakistan. It is a major public health issue whose epidemiology and ecology is strongly related to human lifestyle and activities. Current study aimed at evaluation of Knowledge, attitude and practices regarding dengue fever among people of Karachi, Pakistan. A questionnaire based cross sectional study was conducted between February-April 2022. Questionnaire was prepared and adapted from previous literature available and it was designed on Google forms. This questionnaire was distributed randomly through various social media networks. Questionnaire had four sections which included socio-demographic data of all Participants, questions about knowledge, attitude and practice of dengue fever. Data was evaluated statistically (descriptive statistics) using SPSS software version 25. A total of 140 respondents filled the Google survey form. Out of 140 respondents or participants (42.9%) were male and (57.1%) were female. Majority of participants (80%) were between age group of 15–30 years. Participants have different educational status i.e. undergraduate participants were 57.1% and postgraduate were 32.9%. Majority of participants were single (72.1%) and students (70%). Ninty percent of people reported that they had heard about dengue fever. Dengue as a viral disease was reported by 72.1% but still 50.7% thought that antibiotics are used as treatment of dengue fever. Panadol reduces the intensity of dengue fever was reported by 49.3% respondents. Only 26.4% respondents participated in awareness programs related to dengue fever. Positive perception regarding visit to a physician after getting dengue fever was given by 74.3%. The knowledge about dengue fever was adequate in local community or people of Karachi, Pakistan, while the attitude and practices regarding life style modification requires immediate improvements. Good knowledge doesn't necessarily lead to good practice and positive perception. It is high time that awareness and educational campaigns on dengue fever should be designed by government to improve behavior and practices of prevention and control of dengue fever.