Background: Alzheimer disease (AD) is a progressive neurodegenerative disease caused by many factors. The essential genes and signaling pathways involved in the pathogenesis of AD are still unknown. The purpose of our research is to analyze and screen out potential molecular biomarkers and related signaling pathways.
Methods: We obtained the gene expression profile of GSE18309 from the gene expression Omnibus website. Then, we used the Limma package in Rstudio to screen out differentially expressed genes (DEGs), followed by the corresponding cell signal pathway enrichment using DAVID analysis. Finally, STRING used to obtain the protein-protein interaction (PPI) network and the corresponding hub gene obtained through MCODE of Cytoscape software.
Results: The results showed that a total of 119 upregulated genes and 160 downregulated genes were identified, which met the criteria of |log2 changes| ≥2, adjusted P value <0.01. Through the PPI network, the hub gene module we obtained shows that genes such as GNG13, EDNRB, CHRM3, CCKAR, FFAR4 and TRIO are closely related to AD. The signaling pathway is about signal transducer activity, G-protein coupled receptor activity and transmembrane signaling receptor activity.
Conclusions: In summary, the above-obtained hub gene and the identified signaling pathway will help explore the pathogenesis of AD; and provide new therapeutic targets and prognostic assessment for AD.