This preprint is under consideration at BMC Cardiovascular Disorders. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
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Research article
Gang Li
Xiamen University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3875-2112
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Multiple miRNAs were reported to be associated with sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) in mediating the process of heart failure. However, whether these miRNAs are relevant to chronic heart failure (CHF) is rarely reported.
Methods
This study was designed to explore a new biomarker from the SERCA2a related candidate miRNAs for CHF. A total of 195 blood samples from 84 CHF patients, 86 non-heart failure patients and 25 healthy subjects were collected. The circulating expression of SERCA2a related candidate miRNAs (let-7a-5p, miR-24-3p, miR-25-3p, miR-133a-3p, miR-137a-3p, miR-140-3p, miR-141-3p, miR-142-3p, miR-148a-3p, and miR-153-3p) were detected with qRT-PCR. The area under curve value, correlations between miRNAs and NT-proBNP were applied to determine the relationship between CHF and the candidate miRNAs.
Results
The results showed among the 10 candidate miRNAs, only let-7a-5p, miR-25-3p, and miR-140-3p were significantly increased of CHF patients. However, only miR-140-3p showed the high area under the curve (AUC) value (ROC = 0.8873) and strong positive correlations (r = 0.5113) between miR-140-3p levels and NT-proBNP concentration. The level of miR-140-3p in LVEF < 45% of CHF patients was higher than in LVEF > 45%. However, there was no significant difference between hypertension and non-hypertension of the levels of miR-140-3p in CHF patients. Interestingly, the level of miR-140-3p was higher in NYHA IV CHF patients than in NYHA II or III. More important, the miR-140-3p level also declined when the heart function of NYHA IV patients was improved after treatment.
Conclusions
These results indicated circulation levels of miR-140-3p is related to the severity of chronic heart failure, and miR-140-3p may be an important biomarker for heart failure with reduced ejection fraction.
Keywords
SERCA2a, let-7a-5p, miR-140-3p, miR-25-3p, chronic heart failure
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Reviewer #3 agreed
On 18 Mar, 2021
Reviewer #2 agreed
On 15 Mar, 2021
Reviewer #1 agreed
On 14 Mar, 2021
Review #1 received
Received 14 Mar, 2021
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This preprint is under consideration at BMC Cardiovascular Disorders. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research article
Gang Li
Xiamen University
Corresponding Author
ORCiD: https://orcid.org/0000-0003-3875-2112
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Under Review
Version 1
Posted 10 Mar, 2021
No community comments so far
Review #3 received
Received 02 Apr, 2021
Reviewer #4 agreed
On 19 Mar, 2021
Review #2 received
Received 19 Mar, 2021
Reviewer #3 agreed
On 18 Mar, 2021
Reviewer #2 agreed
On 15 Mar, 2021
Reviewer #1 agreed
On 14 Mar, 2021
Review #1 received
Received 14 Mar, 2021
Editor assigned
On 02 Mar, 2021
Reviewers invited
Invitations sent on 02 Mar, 2021
Submission checks complete
On 02 Mar, 2021
Editor invited
On 02 Mar, 2021
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background
Multiple miRNAs were reported to be associated with sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) in mediating the process of heart failure. However, whether these miRNAs are relevant to chronic heart failure (CHF) is rarely reported.
Methods
This study was designed to explore a new biomarker from the SERCA2a related candidate miRNAs for CHF. A total of 195 blood samples from 84 CHF patients, 86 non-heart failure patients and 25 healthy subjects were collected. The circulating expression of SERCA2a related candidate miRNAs (let-7a-5p, miR-24-3p, miR-25-3p, miR-133a-3p, miR-137a-3p, miR-140-3p, miR-141-3p, miR-142-3p, miR-148a-3p, and miR-153-3p) were detected with qRT-PCR. The area under curve value, correlations between miRNAs and NT-proBNP were applied to determine the relationship between CHF and the candidate miRNAs.
Results
The results showed among the 10 candidate miRNAs, only let-7a-5p, miR-25-3p, and miR-140-3p were significantly increased of CHF patients. However, only miR-140-3p showed the high area under the curve (AUC) value (ROC = 0.8873) and strong positive correlations (r = 0.5113) between miR-140-3p levels and NT-proBNP concentration. The level of miR-140-3p in LVEF < 45% of CHF patients was higher than in LVEF > 45%. However, there was no significant difference between hypertension and non-hypertension of the levels of miR-140-3p in CHF patients. Interestingly, the level of miR-140-3p was higher in NYHA IV CHF patients than in NYHA II or III. More important, the miR-140-3p level also declined when the heart function of NYHA IV patients was improved after treatment.
Conclusions
These results indicated circulation levels of miR-140-3p is related to the severity of chronic heart failure, and miR-140-3p may be an important biomarker for heart failure with reduced ejection fraction.
Figure 1
Figure 2
Figure 3
Figure 4
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