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Original article
Fermented Astragalus in diet improved laying performance, egg quality, antioxidant and immunological status and intestinal microbiota in laying hens
Yingqian Han
Henan Agricultural University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at AMB Express.
In the era of increased antibiotic resistance and ever stricter control on antibiotic use, it is urgent to develop green, safe and non-residue alternatives to antibiotics applied to the poultry industry. To this end, we supplied the potential Lactobacillus Plantarum (L. Plantarum) fermented Astragalus in the diet of laying hens, with a final addition of 3‰. Its effects have been assessed on laying performance, egg quality, antioxidant and immunological status and intestinal microbiota, and are compared to the control group, to the Astragalus group containing 3‰ unfermented Astragalus, and to the L. Plantarum group containing 2% L. Plantarum (1 × 108 CFU/mL). During the second half of the experimental period (15 to 28 days), the egg production rate was significantly higher in the fermented Astragalus group than that in the other groups, with the fermented Astragalus group having the lowest feed conversion ratio. No significant difference (P > 0.05) was observed among treatments on egg quality. Fermented Astragalus-treated hens exhibited significantly increased catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in serum, and reduced malondialdehyde (MDA) in serum. Furthermore, fermented Astragalus supplementation resulted in a significant increase in ileal microbiota abundance relative to control. In conclusion, feeding laying hens with L. Plantarum fermented Astragalus has beneficial effects on production, antioxidant potential, immunity and ileal microbiota. L. Plantarum fermented Astragalus is expected to be a novel feed additive used in poultry production.
Keywords
fermented Astragalus, laying hens, egg quality, antioxidant and immunological status, 16S rRNA, intestinal microbiota
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Peer Review Timeline
CURRENT STATUS: Published
Published
On 31 Aug, 2020
No community comments so far
Editorial decision: Accept
On 16 Aug, 2020
Review #1 received
Received 02 Aug, 2020
Reviewer #1 agreed
On 29 Jul, 2020
Reviewers invited
Invitations sent on 27 Jul, 2020
Editor assigned
On 25 Jul, 2020
Submission checks complete
On 24 Jul, 2020
Editor invited
On 24 Jul, 2020
Version 1
Posted 28 May, 2020
No comments provided
Editorial decision: Minor Revision
On 08 Jul, 2020
Review #2 received
Received 01 Jul, 2020
Review #3 received
Received 01 Jul, 2020
Review #1 received
Received 28 Jun, 2020
Reviewer #3 agreed
On 17 Jun, 2020
Reviewer #1 agreed
On 16 Jun, 2020
Reviewer #2 agreed
On 16 Jun, 2020
Reviewers invited
Invitations sent on 07 Jun, 2020
Editor assigned
On 30 May, 2020
Editor invited
On 29 May, 2020
Submission checks complete
On 23 May, 2020
First submitted
On 20 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Applied & Industrial Microbiology
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This preprint is under consideration at AMB Express. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Original article
Fermented Astragalus in diet improved laying performance, egg quality, antioxidant and immunological status and intestinal microbiota in laying hens
Yingqian Han
Henan Agricultural University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Published
On 31 Aug, 2020
No community comments so far
Editorial decision: Accept
On 16 Aug, 2020
Review #1 received
Received 02 Aug, 2020
Reviewer #1 agreed
On 29 Jul, 2020
Reviewers invited
Invitations sent on 27 Jul, 2020
Editor assigned
On 25 Jul, 2020
Submission checks complete
On 24 Jul, 2020
Editor invited
On 24 Jul, 2020
Version 1
Posted 28 May, 2020
No comments provided
Editorial decision: Minor Revision
On 08 Jul, 2020
Review #2 received
Received 01 Jul, 2020
Review #3 received
Received 01 Jul, 2020
Review #1 received
Received 28 Jun, 2020
Reviewer #3 agreed
On 17 Jun, 2020
Reviewer #1 agreed
On 16 Jun, 2020
Reviewer #2 agreed
On 16 Jun, 2020
Reviewers invited
Invitations sent on 07 Jun, 2020
Editor assigned
On 30 May, 2020
Editor invited
On 29 May, 2020
Submission checks complete
On 23 May, 2020
First submitted
On 20 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Applied & Industrial Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published version at AMB Express.
In the era of increased antibiotic resistance and ever stricter control on antibiotic use, it is urgent to develop green, safe and non-residue alternatives to antibiotics applied to the poultry industry. To this end, we supplied the potential Lactobacillus Plantarum (L. Plantarum) fermented Astragalus in the diet of laying hens, with a final addition of 3‰. Its effects have been assessed on laying performance, egg quality, antioxidant and immunological status and intestinal microbiota, and are compared to the control group, to the Astragalus group containing 3‰ unfermented Astragalus, and to the L. Plantarum group containing 2% L. Plantarum (1 × 108 CFU/mL). During the second half of the experimental period (15 to 28 days), the egg production rate was significantly higher in the fermented Astragalus group than that in the other groups, with the fermented Astragalus group having the lowest feed conversion ratio. No significant difference (P > 0.05) was observed among treatments on egg quality. Fermented Astragalus-treated hens exhibited significantly increased catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in serum, and reduced malondialdehyde (MDA) in serum. Furthermore, fermented Astragalus supplementation resulted in a significant increase in ileal microbiota abundance relative to control. In conclusion, feeding laying hens with L. Plantarum fermented Astragalus has beneficial effects on production, antioxidant potential, immunity and ileal microbiota. L. Plantarum fermented Astragalus is expected to be a novel feed additive used in poultry production.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6