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Polyhydroxyalkanoates (PHAs) from dairy wastewater effluent: bacterial accumulation, structural characterization and physical properties
Olimpia Pepe
University of Naples Federico II
Corresponding Author
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Background to establish bioplastics the real alternative to conventional plastics, high production costs have to be constrained by using different kind of wastewater streams as organic substrate and novel microbial strains as accumulating bacteria with high performance. Volatile fatty acids (VFAs) from effluent of dairy wastewater biodigestion represent a new and cheap feedstock used in this study for biopolymers production through microbial processes.
Results Cupriavidus necator DSM 13513 was particularly able to accumulate PHAs operating in fed-batch mode by limiting oxygen level together with intermittent feeding of carbon source with the maximum PHB accumulated in 48 h without compromising the microbial growth. The complex VFA mixture from digestate did not influence the PHA homopolymer accumulation. In fact, structural characterization by NMR analysis revealed the 3-hydroxybutirrate synthesis (PHB) by C. necator DSM 13513 grown with these different VFAs mixtures. Moreover, the bioplastic disk obtained C. necator DSM 13513 cells grown on VFA from digested dairy waste effluent, presented good thermic properties and low affinity to water.
Conclusions overall results making the digested dairy waste effluent suitable for PHB production for specific bio-based industrial applications.
Keywords
biodigestate, volatile fatty acids, Cupriavidus necator, poly-β-hydroxybutyrate, biopolymer properties
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This is a preprint. It has not completed peer review.
Research
Polyhydroxyalkanoates (PHAs) from dairy wastewater effluent: bacterial accumulation, structural characterization and physical properties
Olimpia Pepe
University of Naples Federico II
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 27 May, 2020
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biotechnology and Bioengineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Background to establish bioplastics the real alternative to conventional plastics, high production costs have to be constrained by using different kind of wastewater streams as organic substrate and novel microbial strains as accumulating bacteria with high performance. Volatile fatty acids (VFAs) from effluent of dairy wastewater biodigestion represent a new and cheap feedstock used in this study for biopolymers production through microbial processes.
Results Cupriavidus necator DSM 13513 was particularly able to accumulate PHAs operating in fed-batch mode by limiting oxygen level together with intermittent feeding of carbon source with the maximum PHB accumulated in 48 h without compromising the microbial growth. The complex VFA mixture from digestate did not influence the PHA homopolymer accumulation. In fact, structural characterization by NMR analysis revealed the 3-hydroxybutirrate synthesis (PHB) by C. necator DSM 13513 grown with these different VFAs mixtures. Moreover, the bioplastic disk obtained C. necator DSM 13513 cells grown on VFA from digested dairy waste effluent, presented good thermic properties and low affinity to water.
Conclusions overall results making the digested dairy waste effluent suitable for PHB production for specific bio-based industrial applications.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Due to technical limitations, full-text HTML conversion of this manuscript could not be completed. However, the manuscript can be downloaded and accessed as a PDF.