See the published version of this article at Journal of Cotton Research.
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Zhongli Zhou
Chinese Academy of Agricultural Sciences Cotton Research Institute
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1900-5798
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of Cotton Research.
Background
Segregation distortion (SD) is a phenomenon common among stable or segregating populations, and the principle behind it still puzzles many researchers. The F2:3 progenies developed from the wild cotton species of the D genomes were used to investigate the possible plant transcription factors within the segregation distortion regions (SDRs). A consensus map was developed between two maps from the four D genome, map A derived from an F2:3 progenies of Gossypium klotzschianum and G. davidsonii while Map B from G. thurberi and G. trilobum F2:3 generations. In each map 188 individual plants were used.
Results
The consensus linkage map had 1,492 markers across the 13 linkage groups; with a map size of 1,467.445 cM and average marker distance of 1.0370 cM. Chromosome D502 had the highest percentage of SD with 58.621% followed by Chromosome D507 with 47.887%. Six thousand and thirty eight genes were mined within the SDRs on chromosome D502 and D507 of the consensus map. Within chromosome D502 and D507, 2,308 and 3,730 genes were mined, respectively and were found to belong to 1,117 domains out of which 622 domains were common across the two chromosomes. Moreover, the first 9 domains were members of the plant resistance genes (R genes), while Pkinase; Protein kinase domain (PF00069) was the dominant group with 188 genes. Further analysis on the dominant domains revealed that 287 miRNAs were found to target various genes, such as the gr-miR398, gra-miR5207, miR164a, miR164b, miR164c among others, which have been found to target top-ranked stress-responsive transcription factors such as NAC genes. Moreover, some of the stress responsive cis-regulatory elements were also detected. Furthermore, RNA profiling of the genes from the dominant family showed that higher numbers of genes were highly upregulated under salt and osmotic stress conditions in addition their higher expression at different stages fiber development.
Conclusion
The result obtained provided an indication of the important role of the SDRs in the evolution of significant genes in plants.
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Published
On 06 Dec, 2020
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Editorial decision: Accept
On 20 Oct, 2020
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On 18 Oct, 2020
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On 17 Oct, 2020
Editor invited
On 17 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 11 Oct, 2020
Review #1 received
Received 30 Sep, 2020
Review #2 received
Received 29 Sep, 2020
Reviewer #2 agreed
On 26 Sep, 2020
Reviewers invited
Invitations sent on 23 Sep, 2020
Reviewer #1 agreed
On 23 Sep, 2020
Editor assigned
On 21 Sep, 2020
Submission checks complete
On 20 Sep, 2020
Editor invited
On 20 Sep, 2020
Version 1
Posted 27 May, 2020
No comments provided
Editorial decision: Major revision
On 31 Aug, 2020
Reviewer #4 agreed
On 24 Aug, 2020
Review #3 received
Received 22 Aug, 2020
Review #2 received
Received 22 Aug, 2020
Reviewer #3 agreed
On 16 Aug, 2020
Reviewer #2 agreed
On 03 Jun, 2020
Review #1 received
Received 02 Jun, 2020
Reviewers invited
Invitations sent on 21 May, 2020
Reviewer #1 agreed
On 21 May, 2020
First submitted
On 20 May, 2020
Editor assigned
On 20 May, 2020
Submission checks complete
On 19 May, 2020
Editor invited
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This preprint is under consideration at Journal of Cotton Research. A preprint is a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Learn more about In Review
Research
Zhongli Zhou
Chinese Academy of Agricultural Sciences Cotton Research Institute
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1900-5798
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
Peer Review Timeline
CURRENT STATUS: Published
Published
On 06 Dec, 2020
No community comments so far
Editorial decision: Accept
On 20 Oct, 2020
Editor assigned
On 18 Oct, 2020
Submission checks complete
On 17 Oct, 2020
Editor invited
On 17 Oct, 2020
No comments provided
Editorial decision: Minor revision
On 11 Oct, 2020
Review #1 received
Received 30 Sep, 2020
Review #2 received
Received 29 Sep, 2020
Reviewer #2 agreed
On 26 Sep, 2020
Reviewers invited
Invitations sent on 23 Sep, 2020
Reviewer #1 agreed
On 23 Sep, 2020
Editor assigned
On 21 Sep, 2020
Submission checks complete
On 20 Sep, 2020
Editor invited
On 20 Sep, 2020
Version 1
Posted 27 May, 2020
No comments provided
Editorial decision: Major revision
On 31 Aug, 2020
Reviewer #4 agreed
On 24 Aug, 2020
Review #3 received
Received 22 Aug, 2020
Review #2 received
Received 22 Aug, 2020
Reviewer #3 agreed
On 16 Aug, 2020
Reviewer #2 agreed
On 03 Jun, 2020
Review #1 received
Received 02 Jun, 2020
Reviewers invited
Invitations sent on 21 May, 2020
Reviewer #1 agreed
On 21 May, 2020
First submitted
On 20 May, 2020
Editor assigned
On 20 May, 2020
Submission checks complete
On 19 May, 2020
Editor invited
On 19 May, 2020
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Journal of Cotton Research.
Background
Segregation distortion (SD) is a phenomenon common among stable or segregating populations, and the principle behind it still puzzles many researchers. The F2:3 progenies developed from the wild cotton species of the D genomes were used to investigate the possible plant transcription factors within the segregation distortion regions (SDRs). A consensus map was developed between two maps from the four D genome, map A derived from an F2:3 progenies of Gossypium klotzschianum and G. davidsonii while Map B from G. thurberi and G. trilobum F2:3 generations. In each map 188 individual plants were used.
Results
The consensus linkage map had 1,492 markers across the 13 linkage groups; with a map size of 1,467.445 cM and average marker distance of 1.0370 cM. Chromosome D502 had the highest percentage of SD with 58.621% followed by Chromosome D507 with 47.887%. Six thousand and thirty eight genes were mined within the SDRs on chromosome D502 and D507 of the consensus map. Within chromosome D502 and D507, 2,308 and 3,730 genes were mined, respectively and were found to belong to 1,117 domains out of which 622 domains were common across the two chromosomes. Moreover, the first 9 domains were members of the plant resistance genes (R genes), while Pkinase; Protein kinase domain (PF00069) was the dominant group with 188 genes. Further analysis on the dominant domains revealed that 287 miRNAs were found to target various genes, such as the gr-miR398, gra-miR5207, miR164a, miR164b, miR164c among others, which have been found to target top-ranked stress-responsive transcription factors such as NAC genes. Moreover, some of the stress responsive cis-regulatory elements were also detected. Furthermore, RNA profiling of the genes from the dominant family showed that higher numbers of genes were highly upregulated under salt and osmotic stress conditions in addition their higher expression at different stages fiber development.
Conclusion
The result obtained provided an indication of the important role of the SDRs in the evolution of significant genes in plants.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
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