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Research Article
Guoping Chen
Chongqing University
Corresponding Author
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The process of plant senescence is complex and highly coordinated, and is regulated by many endogenous and environmental signals. Ethylene and jasmonic acid are well-known senescence inducers, but their molecular mechanisms for inducing leaf senescence have not been fully elucidated. Here, we studied a receptor gene downstream of an ethylene signal transduction pathway, ETHYLENE RESPONSE FACTOR F5 (SlERF.F5). The silence of SlERF.F5 causes accelerated senescence induced by age, darkness, ethylene, and jasmonic acid. However, overexpression of SlERF.F5 may delay leaf senescence. We further found that silencing of SlERF.F5 inhibited the expression of chlorophyll-related genes CHLH, CHLM, POR, CAO1, GUN4, PPH, SGR1, RBCS, and AUREA genes, and the light-responsive RBCS and LHCA1 gene. Moreover, silencing of SlERF.F5 increases the sensitivity of SlERF.F5-RNAi lines to ethylene and jasmonic acid compared to wild type. In the dark-induced aging experiment, the qRT-PCR analysis showed the expression levels of genes related to the ethylene biosynthesis pathway and the jasmonic acid signaling pathway in SlERF.F5-RNAi lines increased compared with wild type. Yeast two-hybrid experiments showed that SlERF.F5 and SlMYC2 (a transcription factor downstream of the JA receptor) can interact physically, thereby mediating the role of SlERF.F5 in jasmonic acid-induced leaf senescence. Collectively, our research provides new insights into how ethylene and jasmonic acid promote leaf senescence in tomatoes.
Keywords
SlERF.F5, leaf senescence, ethylene, jasmonate, SlMYC2, tomato
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This is a list of supplementary files associated with this preprint. Click to download.
- Fig.S1.tif
Silencing of SlERF.F5 shows increased sensitivity to ethylene (a) The seeds after seven days of germination were treated with 0 μM, 5 μM, 10 μM ACC WT, and RNAi10, RNAi13, RNAi16 lines. (b-d) The root length, hypocotyl, and fresh weight of (a)-treated tomato seedlings were measured. (e-f) qRT-PCR was used to determine the expression levels of ACO1, ACS2 in the ethylene biosynthesis pathway of WT and RNAi10, RNAi13, and RNAi16 lines after ACC treatment 0 μM and 10 μM. The data represent the mean from three replicates with three biological repeats. *, indicate P < 0.05, between the wild type and others by t-test. Error bars indicate SE.
- Fig.S2.tif
Silencing of SlERF.F5 shows increased sensitivity to jasmonic acid (a) The seeds after seven days of germination were treated with 0 μM, 10 μM, 20 μM, 50 μM MeJA WT, and RNAi10, RNAi13, RNAi16 lines. (b-d) The root length, hypocotyl and fresh weight of (a)-treated tomato seedlings were measured. The data represent the mean from three replicates with three biological repeats. *, indicate P < 0.05, between the wild type and others by t-test. Error bars indicate SE.
- supplementary.docx
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A new preprint design is coming!
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This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Research Article
Guoping Chen
Chongqing University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
View the published article at Plant Cell Reports.
Version 1
Posted 12 Apr, 2021
No community comments so far
Metrics
Comments: 0
PDF Downloads: ...
HTML Views: ...
Subject Areas
Biotechnology and Bioengineering
License:
This work is licensed under a CC BY 4.0 License. Read Full License
View the published article at Plant Cell Reports.
The process of plant senescence is complex and highly coordinated, and is regulated by many endogenous and environmental signals. Ethylene and jasmonic acid are well-known senescence inducers, but their molecular mechanisms for inducing leaf senescence have not been fully elucidated. Here, we studied a receptor gene downstream of an ethylene signal transduction pathway, ETHYLENE RESPONSE FACTOR F5 (SlERF.F5). The silence of SlERF.F5 causes accelerated senescence induced by age, darkness, ethylene, and jasmonic acid. However, overexpression of SlERF.F5 may delay leaf senescence. We further found that silencing of SlERF.F5 inhibited the expression of chlorophyll-related genes CHLH, CHLM, POR, CAO1, GUN4, PPH, SGR1, RBCS, and AUREA genes, and the light-responsive RBCS and LHCA1 gene. Moreover, silencing of SlERF.F5 increases the sensitivity of SlERF.F5-RNAi lines to ethylene and jasmonic acid compared to wild type. In the dark-induced aging experiment, the qRT-PCR analysis showed the expression levels of genes related to the ethylene biosynthesis pathway and the jasmonic acid signaling pathway in SlERF.F5-RNAi lines increased compared with wild type. Yeast two-hybrid experiments showed that SlERF.F5 and SlMYC2 (a transcription factor downstream of the JA receptor) can interact physically, thereby mediating the role of SlERF.F5 in jasmonic acid-induced leaf senescence. Collectively, our research provides new insights into how ethylene and jasmonic acid promote leaf senescence in tomatoes.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
This is a list of supplementary files associated with this preprint. Click to download.
- Fig.S1.tif
Silencing of SlERF.F5 shows increased sensitivity to ethylene (a) The seeds after seven days of germination were treated with 0 μM, 5 μM, 10 μM ACC WT, and RNAi10, RNAi13, RNAi16 lines. (b-d) The root length, hypocotyl, and fresh weight of (a)-treated tomato seedlings were measured. (e-f) qRT-PCR was used to determine the expression levels of ACO1, ACS2 in the ethylene biosynthesis pathway of WT and RNAi10, RNAi13, and RNAi16 lines after ACC treatment 0 μM and 10 μM. The data represent the mean from three replicates with three biological repeats. *, indicate P < 0.05, between the wild type and others by t-test. Error bars indicate SE.
- Fig.S2.tif
Silencing of SlERF.F5 shows increased sensitivity to jasmonic acid (a) The seeds after seven days of germination were treated with 0 μM, 10 μM, 20 μM, 50 μM MeJA WT, and RNAi10, RNAi13, RNAi16 lines. (b-d) The root length, hypocotyl and fresh weight of (a)-treated tomato seedlings were measured. The data represent the mean from three replicates with three biological repeats. *, indicate P < 0.05, between the wild type and others by t-test. Error bars indicate SE.
- supplementary.docx
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